31results about How to "Increase the reproduction factor" patented technology

The invention provides a method for improving the yield of saponin in panax notoginseng tissue culture seedlings by applying jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding system of panax notoginseng tissue culture seedlings, applying jasmonic acid methyl ester to pseudo-ginseng tissue culture seedlings, cultivating pseudo-ginseng tissue culture seedlings and applying and spraying jasmonic acid methyl ester to the leaf surfaces of pseudo-ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied jasmonic acid methyl ester can effectively improve the yield of total panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main monomer ginsenoside by 2-5 times. According to the method for improving yield of saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a solid cultivation method, expensive fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for mass production of notoginsenoside.

The invention provides a tissue culture method of tilia mongolica seedlings. The method comprises the following steps of (1) taking tilia mongolica seeds for sterilization treatment; (2) removing seedcoats of the sterilized tilia mongolica seeds and inoculating the seeds into an induction culture medium for induction culture to obtain adventitious buds; (3) inoculating the adventitious buds intoa subculture medium for subculture to obtain subculture seedlings; (4) inoculating the subculture seedlings into a rooting culture medium for rooting culture to obtain rooting seedlings. According tothe method, proliferation is carried out in the proliferation period at the speed rate of 3.8 times, and the rooting rate is 86%. The method can achieve culture of massive regenerated tilia mongolicaseedlings at a time, solve the problems of seed dormancy and slow growth of seedlings, and avoid the spread of plant diseases and insect pests caused by a cutting mode. The method can obviously increase the breeding coefficient of tilia mongolica and shorten the breeding cycle.

The invention discloses a high-efficiency breeding method for sunflower plants. The mature and plump sunflower seeds are taken as initial materials, and the surface is sterilized; the sterilized seeds are inoculated into a germination medium, and cultivated under light to obtain aseptic seedlings ; Under aseptic conditions, the stem section of the aseptic seedling of goldenflower anemone is cut into sections, and inoculated in the adventitious bud induction medium. After light cultivation, adventitious bud clusters are induced at the incisions at both ends of the stem section; The stem section was transferred to the elongation medium for the elongation culture of adventitious buds; when the adventitious buds grew to complete leaves, they were transferred to the rooting medium for rooting culture, and after light culture, a test tube of goldenflower sunflower with strong root system was obtained Seedling. The breeding of sunflower seedlings can be carried out without the need for seedling breeding, thereby eliminating the time limit for raising sunflower seedlings, improving the seedling breeding coefficient, and laying the foundation for the large-scale breeding and variety improvement research of good golden sunflower lines in the later period. Base.

The invention provides a phalaenopsis sterile root propagation method, belongs to the technical field of seedlings of plant cultivation, and particularly relates to a method for cultivating seedlings of red phalaenopsis flower which is prepared by proliferating cluster buds induced by phalaenopsis sterile roots. According to the invention, plants of excellent pest-free phalaenopsis are selected; flower peduncle axillary bud stems in length of 2 cm are cut off from the robust mother plants to be used as an explant material; after being subjected to one month of material primary generation induction cultivation, grown-out axillary buds of the explant material are cut off and subjected to 40-day rooting cultivation; roots are cut off from the axillary buds, and are inoculated to a cluster bud induction culture medium; a plurality of buds can be grown out from the cutting points after 20 days, the cultivation is continued, and a large number of cluster buds can be grown out; the cluster buds can be cut off for rooting cultivation after being grown up, and a large number of cluster buds can be obtained again by continuously cultivating the sterile roots. The method has the advantages that a large number of bluster buds can be obtained by proliferation of the cluster buds from induced sterile roots to realize propagation of the phalaenopsis, so that the industrialized production of the phalaenopsis is realized; by the adoption of the method provided by the invention, the breeding coefficient is greatly improved, the breeding period is shortened, and the survival rate can reach more than 90%.

The invention provides a tissue-culture rapid-breeding method for stewartia sinensis. The tissue-culture rapid-breeding method for the stewartia sinensis comprises the steps of explant selection and disinfection, cluster bud induction, cluster bud proliferation, rooting culture, strong seedling culture and seedbed culture. Compared with the traditional seedling culture method of the stewartia sinensis, the tissue-culture rapid-breeding method for the stewartia sinensis has the following advantages: the method is not limited by the number and the germination rate of of stewartia sinensis seeds; annual young shoots of the stewartia sinens are subjected to tissue culture to obtain stewartia sinensis seedlings, and the stewartia sinensis seedlings are transplanted into seedbeds to be continuously cultured, thereby obtaining stewartia sinensis transplanted seedlings; the tissue-culture rapid-breeding method for the stewartia sinensis can effectively improve the breeding coefficient and retains the excellent hereditary character of the stewartia sinensis, the rate of survival of seedlings is as high as 95%, the rate of survival of field transplated seedlings is as high as 80%, and large-scale production and field large-area forestation growth of stewartia sinensis nursery stocks can be achieved; and the invention provides technical supports for effective protection of the stewartia sinensis which is the rare and endangered plant.

The invention relates to the technical field of huazhonglou seedling propagation, in particular to a method for fast-growing rhizome seedlings of huazhonglouzi without destroying the original environment. The method of the present invention includes: (1) selecting and planting more than 4 years of healthy and pest-free Huachonglou plants; (2) cutting off the rhizome terminal buds and all the above-ground parts; (3) removing the rhizomes left in the soil or the bare ground Cut the cut surface of the rhizome; (4) Disinfect the cut and dry it; (5) Dip the root of the Huazhonglou rhizome with the cut with GGR amino acid solution, spray boron fertilizer aqueous solution at the same time, dry it, and cover it with humus ; (6) Field management of the rhizomes of Huachonglou covered with humus soil; (7) In the autumn of the second year, many strong seedlings of sub-rhizomes have grown on the mother rhizomes of Huachonglou; (8) Picking (9) Disinfect the seedlings of the sub-rhizome, dry and then transplant; (10) Harvest after four years of cultivation. The invention has fast seedling emergence and high survival rate, solves the problem that seedlings are not easy to obtain in the large-scale planting of Huazhonglou, and has important production value and application value.

The invention discloses an efficient tilia miqueliana strain culture method which comprises the following steps: cutting stem segments with axillary buds as explants, sterilizing surfaces, inoculatinginto a primary culture medium, and performing culture; cutting the axillary buds, inoculating into a proliferation medium, and inducing adventitious buds from the axillary buds; separating the adventitious buds into single buds, and inoculating into an elongation culture medium; moving the elongated adventitious buds outside a tissue culture chamber, and strengthening seedlings under a natural light condition; washing the elongated buds after seedling strengthening, cutting into appropriate micro cut segments, and performing rooting pretreatment; and performing planting fixation on morphological lower ends of the segments treated with rooting promotion liquid into a nutrient medium, and performing grown seedling culture on tilia miqueliana plants in a greenhouse. By adopting the method, the reproductive process, the rooting process and the strengthening process of tilia miqueliana elongated seedlings are combined, so that the proliferation coefficient of the tilia miqueliana is increased, the survival rate of tissue culture seedlings is increased, the seed seedling proliferation process is accelerated, and the industrial seedling culture of the tilia miqueliana is facilitated.

The invention discloses a method for tissue culture regeneration of Danish hibiscus, and relates to the technical field of Danish hibiscus. The invention comprises the following steps: taking the young and tender stem section with axillary buds of an excellent strain of Danish hibiscus as an explant, and after surface disinfection Adventitious bud induction and proliferation culture were carried out; when the adventitious buds elongated to 2-3 cm and accompanied by 2-4 complete leaves, adventitious roots were induced inside or outside the bottle, and finally complete Danish hibiscus regenerated plants were obtained. The beneficial effect of the present invention is that: the present invention is of great significance to the research on large-scale propagation and variety improvement of Danish hibiscus excellent strains.

The invention discloses a method for culturing strawberry virus-free seedlings through a tissue culture technology. The method includes the steps of firstly, selecting and sterilizing an explant, wherein unopened buds of a strawberry plant are collected, after the buds are sterilized and processed with alcohol and corrosive sublimate, anther is taken out, and the explant is obtained; secondly, inducing cluster buds, wherein the explant obtained in the first step is inoculated in a culture medium M1 to be cultured in the dark for 8-10 days and then cultured in light for 80-90 days till the cluster buds are produced, and the bud height of the cluster buds is not smaller than 2 cm; thirdly, transferring the cluster buds for culture, wherein the cluster buds obtained in the second step are transferred into a culture medium M2 to be cultured for 15-25 days till the cluster buds grow to strawberry seedlings which are 3-5 cm high, and then the strawberry seedlings are transferred into a culture medium M3 to be subjected to rooting culture to obtain the strawberry virus-free seedlings. The applied culture media are simple in formula, the tissue culture process is simple, the virus-free rate reaches 100%, the reproduction coefficient is high, the transplanting survival rate is high, seedlings are neat, later unified transplanting and planting management is convenient, and industrial production can be achieved.