The invention provides a method for improving the yield of
saponin in
panax notoginseng tissue culture seedlings by applying
jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding
system of
panax notoginseng tissue culture seedlings, applying
jasmonic acid methyl ester to pseudo-
ginseng tissue culture seedlings, cultivating pseudo-
ginseng tissue culture seedlings and applying and spraying
jasmonic acid methyl ester to the leaf surfaces of pseudo-
ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied
jasmonic acid methyl ester can effectively improve the yield of total
panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main
monomer ginsenoside by 2-5 times. According to the method for improving yield of
saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a
solid cultivation method, expensive
fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the
contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for
mass production of notoginsenoside.