31results about How to "Increase the reproduction factor" patented technology

The invention provides a method for improving the yield of saponin in panax notoginseng tissue culture seedlings by applying jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding system of panax notoginseng tissue culture seedlings, applying jasmonic acid methyl ester to pseudo-ginseng tissue culture seedlings, cultivating pseudo-ginseng tissue culture seedlings and applying and spraying jasmonic acid methyl ester to the leaf surfaces of pseudo-ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied jasmonic acid methyl ester can effectively improve the yield of total panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main monomer ginsenoside by 2-5 times. According to the method for improving yield of saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a solid cultivation method, expensive fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for mass production of notoginsenoside.

The invention relates to a core branch scion two-surface grafting method of growth period stopped mulberry, wherein the core stopping strips during mulberry growing period are utilized for the grafting resource, the mated single bud bough double cutting suface grafting method is provided, the grafting technology can be applied into the long period of time after the end of grafting process by using winter strips as ingraftment. The method has the advantages of easy ingraftment acquisition, small amount of consumption, easiness in operation, high work efficiency and high survival rate.

A method for breed strawberry seedlings save labor includes such steps as propagating, refrigerate, classifying, transplanting and activating seedlings. The invention improves the breeding coefficientof seedlings, promotes flower bud differentiation, and simultaneously improves the consistency of seedling quality and seedling age. This method is easy to operate, easy to master, repeatable, time-saving, labor-saving, easy to classify and centralized management, perfecting the shortcomings of conventional strawberry seedling.

The invention provides a tissue culture method of morus mongolica seedlings. The method comprises the following steps of taking morus mongolica dormant buds as explant materials and MS as a basic culture medium and adding 6-BA, 2,4-D, IBA, GA3, PVP-40 and other substances with different concentrations into the culture medium; sequentially carrying out subculture, proliferation culture and rootingculture on the morus mongolica explants to obtain complete plants. By adopting the method, a large number of regenerated morus mongolica seedlings can be obtained, and the problems of variation and slow growth caused by seed propagation and spread of plant diseases and insect pests resulted from a cutting mode are solved. The method can obviously increase the morus mongolica breeding coefficient and shorten the breeding cycle. According to the method, proliferation is carried out at the speed rate of 9.3 times in the proliferation period, and the rooting rate is 100%.

The invention provides a phalaenopsis sterile root propagation method, belongs to the technical field of seedlings of plant cultivation, and particularly relates to a method for cultivating seedlings of red phalaenopsis flower which is prepared by proliferating cluster buds induced by phalaenopsis sterile roots. According to the invention, plants of excellent pest-free phalaenopsis are selected; flower peduncle axillary bud stems in length of 2 cm are cut off from the robust mother plants to be used as an explant material; after being subjected to one month of material primary generation induction cultivation, grown-out axillary buds of the explant material are cut off and subjected to 40-day rooting cultivation; roots are cut off from the axillary buds, and are inoculated to a cluster bud induction culture medium; a plurality of buds can be grown out from the cutting points after 20 days, the cultivation is continued, and a large number of cluster buds can be grown out; the cluster buds can be cut off for rooting cultivation after being grown up, and a large number of cluster buds can be obtained again by continuously cultivating the sterile roots. The method has the advantages that a large number of bluster buds can be obtained by proliferation of the cluster buds from induced sterile roots to realize propagation of the phalaenopsis, so that the industrialized production of the phalaenopsis is realized; by the adoption of the method provided by the invention, the breeding coefficient is greatly improved, the breeding period is shortened, and the survival rate can reach more than 90%.

The invention belongs to the technical field of grafting and particularly discloses a Hongjiang orange seedling summer grafting cultivation method. A Hongjiang orange scion and a stock are grafted indoors and moved to a prearranged greenhouse to be planted, the greenhouse is sequentially covered with an insect-proof net, a waterproof thin film and a sunshade net from inside to outside, a transplanted grafted seedling is watered with sufficient rooting water, and then fertilizer and water management and disease, pest and weed management are conducted. A rise grafting method is adopted, in other words, the qualified and healthy stock is selected for seedling grafting in a laboratory, meanwhile, the facility greenhouse is utilized, the waterproof, sun protection, windproof and moisturizing purposes are achieved, and the survival rate of the seedling is guaranteed. According to the technology, a branch fully growing in a parent material orchard in summer is fully used as the scion, and the propagation coefficient of the seedling is increased.

The invention discloses a high-efficiency propagation method of a hibiseu manihot plant. The method comprises the following steps: collecting mature and full seeds of hibiseu manihot as an initial material, and performing the surface disinfection; inoculating the disinfected seeds into a germination culture medium, performing the lighting culture, and obtaining aseptic seedlings; under aseptic condition, cutting stems of the aseptic seedlings of the hibiseu manihot, inoculating into an adventitious bud induction culture medium, inducing adventitious buds at incisions on two ends of the stem after lighting culture; transferring the stems with the adventitious buds into an elongation culture medium, and performing the elongation culture of the adventitious buds; and when a full leaf of the adventitious bud grows, transferring the adventitious buds into a rooting culture medium, performing the rooting culture, and after the lighting culture, acquiring test-tube seedlings of the hibiseu manihot with strong root system. By adopting the method, the propagation of the seedlings of the hibiseu manihot does not need the seed seedling growth, so that the time limit of the hibiseu maniht seedling is solved, the seedling propagation coefficient is increased, and a foundation is set for the large-scale propagation and variety improvement research of the later excellent plants of the hibiseumanihot.

The invention discloses a tissue culture regeneration method of Denmark hibiscus, and relates to the technical field of the Denmark hibiscus. The tissue culture regeneration method comprises the following steps of taking a current-year tender stem segment with axillary buds of an excellent strain of the Denmark hibiscus as an explant, carrying out surface disinfection, then carrying out adventitious bud induction and multiplication culture, and when adventitious buds extend to 2-3cm and have 2-4 complete leaves, carrying out in-bottle or out-bottle adventitious root induction to finally obtain a complete Denmark hibiscus regenerated plant. The tissue culture regeneration method disclosed by the invention has the beneficial effects that the tissue culture regeneration method is of great significance to large-scale propagation and variety improvement research of the excellent strain of the Denmark hibiscus.

The invention discloses a method for culturing strawberry virus-free seedlings through a tissue culture technology. The method includes the steps of firstly, selecting and sterilizing an explant, wherein unopened buds of a strawberry plant are collected, after the buds are sterilized and processed with alcohol and corrosive sublimate, anther is taken out, and the explant is obtained; secondly, inducing cluster buds, wherein the explant obtained in the first step is inoculated in a culture medium M1 to be cultured in the dark for 8-10 days and then cultured in light for 80-90 days till the cluster buds are produced, and the bud height of the cluster buds is not smaller than 2 cm; thirdly, transferring the cluster buds for culture, wherein the cluster buds obtained in the second step are transferred into a culture medium M2 to be cultured for 15-25 days till the cluster buds grow to strawberry seedlings which are 3-5 cm high, and then the strawberry seedlings are transferred into a culture medium M3 to be subjected to rooting culture to obtain the strawberry virus-free seedlings. The applied culture media are simple in formula, the tissue culture process is simple, the virus-free rate reaches 100%, the reproduction coefficient is high, the transplanting survival rate is high, seedlings are neat, later unified transplanting and planting management is convenient, and industrial production can be achieved.

The invention discloses a Chinese scholartree hard branch cutting breeding method, and relates to the technical field of vegetative breeding in the forestry science. The method comprises the steps of1) establishing a cutting orchard, collecting scions in the spring, hiding in sand, in the middle of April, cutting to 6-8 cm, sealing two ends with waxes, using Chinese scholartree seedlings as rootstocks, and building a happy cutting orchard; 2) collecting and processing, bundling after branch cutting, controlling the length of the cuttings to be 8-9 cm, soaking the cuttings in NAA 300*10 6 mg /L + ABT1 200*10 6 mg / L of a solution, and soaking for 12h; 3) setting up a germination pool, and spreading river sand on the bottom of the germination pool; 4) germinating, putting the cuttings vertically into the germination pool, filling the surrounding and the upper portion of the river with sand, sprinkling water, covering a ground film, and setting a circumference fence; 5) after germination for 40 days, excavating the cuttings, and rinsing to be clean; 6) conducting field planting, ditching according to the row spacing of 30 cm*20 cm, and planting the cuttings of 5) in the ditches ina slightly inclined manner, wherein the upper end is exposed to the position 2 cm above the ground, the water is poured through, watering is conducted each 3-5d, pouring is conducted three times in arow, and the normal management is entered. The method has simple management and has the advantages of improving the breeding coefficient of the Chinese scholartree and the cutting survival rate and reducing the cost.