Aquaculture seed reproduction method for micro seed tubers of konjac

A hydroponic and miniature technology, applied in the fields of botanical equipment and methods, cultivation, soilless cultivation, etc., can solve the problem of high requirements for cultivation conditions, long period of producing high-quality seed taro, reduction of tissue culture seedling survival rate and taro formation rate, etc. problems, to achieve the effect of shortening the breeding cycle, shortening the breeding cycle, high survival rate and high taro setting rate

Inactive Publication Date: 2015-03-18
云南省农业科学院生物技术与种质资源研究所 +2
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the high requirements of the cultivation management technology operation and cultivation conditions of konjac tissue culture seedlings in the existing konjac miniature seedling tissue culture, which is easy to cause the survival rate and the fruiting rate of the tissue culture seedlings to be greatly reduced, and Technical defects and deficiencies in the long cycle of producing high-quality seed taro

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 currently existing plant tissue culture technology carries out plant tissue culture to konjac

[0015] Konjac is carried out to plant tissue culture and can adopt following steps:

[0016] (1) Selection of explants, selection of konjac bulbs with well-developed, undamaged and smooth seed coats, slightly elliptical spherical shape, shallow bud nests, and a weight of about 250 g as explants;

[0017] (2) Cleaning and germination of the explants, clean the epidermis and bud nests of the explants (bulbs) with tap water, and then perform germination treatment in a clean environment at 28°C;

[0018] (3) When the konjac corm terminal bud grows to 2-3cm, cut the terminal bud, and use a conventional disinfection method to disinfect the material. The conventional disinfection method can be carried out as follows: clean the cut terminal bud with tap water, Disinfect the surface with 70% alcohol for 1 second → disinfect with 0.1% mercuric chloride for 5 minutes → rin...

Embodiment 2

[0022] The hydroponic breeding method of embodiment 2 konjac miniature taro

[0023] (1) Hydroponics is carried out indoors. First cut the unrooted konjac tissue culture seedlings produced by conventional tissue culture with a seedling height of 4cm-5cm. When cutting, use a sharp scalpel to cut the petiole of the seedling and the tumor at the base. Divide the callus, then wash away the culture medium at the base of the tissue culture seedling with tap water, soak it with 5% carbendazim for 2-3 minutes, wrap the base of the plant with a sponge as a fixer, and implant the plant. Row spacing is 5.2 centimeters × 5.2 centimeters in the tray, each tray is planted with 100 plants, and the tray is placed on the water surface of the container (pan) filled with tap water, and then the container (pan) is placed on the bench-top culture frame, The distance between each layer of mesa is 35cm-40cm. During the hydroponics period, the indoor temperature is controlled at 10-15°C at night, and...

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Abstract

The invention discloses an aquaculture seed reproduction method for micro seed tubers of konjac, belonging to the technical field of agriculture breeding and propagation. The aquaculture seed reproduction method comprises the steps of segmenting nodular calluses of a petiole and a base thereof of a konjac tissue culture seedling by using a knife, wherein the konjac tissue culture seedling grows until reaching the height of 4-5cm and does not root; washing the tissue culture seedling with water and wrapping the basal of the tissue culture seedling with sponge, planting in a hole plug and carrying out water-planting indoors by using tap water, spraying a nutrient solution once every 7 days after the tissue culture seedling grows for 7 days, wherein the mass ratio of N to P to K to Ca to Mg in the nutrient solution is 23:1:21:4:1, the total concentration of the nutrient solution is 225mg / L, the indoor temperature at night is 10-15 DEG C, the indoor temperature in the daylight is 20-25 DEG C, and the illumination is scattered light; carrying out water-planting for 3 months and then stopping water-planting to ensure that plants naturally suffers from sprout tumble, namely, the mature micro seed tubers can be harvested. According to the method, the micro seed tubers with the weight of 1.5g on average of the konjac can be bred, thus the propagation period of excellent seed tubers of the konjac is greatly shortened, the production cost is low, and the survival rate and the tuber yield reach 85-90 percent.

Description

technical field [0001] The invention relates to a technique for hydroponic breeding of konjac miniature taro, which belongs to the technical field of agricultural breeding and breeding. technical background [0002] Konjac (konjac) is a perennial herbaceous plant of the Araceae family Konjac. It mainly uses bulbs for asexual reproduction. Konjac has been able to breed excellent seedlings through plant tissue culture biotechnology and transplant them to seedbeds for breeding. The scarcity of fine taro seeds provides an effective solution. However, the cultivation and management of konjac tissue culture seedlings is a very delicate work. A little carelessness in technical operation and cultivation conditions will greatly reduce the survival rate and fruiting rate of tissue culture seedlings, and the production cycle of high-quality seedlings is long, and it takes A growth cycle (6-8 months). [0003] Therefore, finding a kind of method that can not only shorten the breeding ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00
Inventor 王玲尹桂芳马继琼孙道旺孙涛陈燕萍蒋晓云高凡姜德有徐云杨奕
Owner 云南省农业科学院生物技术与种质资源研究所
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