A kind of method for efficient breeding of goldenflower sunflower plants

A technology of sunflower and plants, which is applied in the field of high-efficiency breeding of sunflower plants, can solve problems such as no reports of sunflower sunflower, and achieve the effects of improving seedling breeding coefficient, fast regeneration speed, and convenient material collection

Active Publication Date: 2021-04-06
河南芙罗拉农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research on the breeding of goldenflower sunflower using plant tissue culture technology.

Method used

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  • A kind of method for efficient breeding of goldenflower sunflower plants
  • A kind of method for efficient breeding of goldenflower sunflower plants
  • A kind of method for efficient breeding of goldenflower sunflower plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method for the high-efficiency breeding of the sunflower sunflower plant of the present embodiment may further comprise the steps:

[0028] (1) Take mature and plump sunflower seeds, wash them with running water for 10 minutes, sterilize them with 75% absolute ethanol for 60 seconds in a sterile operating table, then sterilize them with 25% sodium hypochlorite solution for 5 minutes, and then use a 600W ultrasonic instrument to sonicate them 4min, finally rinsed with sterile water 6 times, filter paper to dry the moisture on the seed surface, set aside.

[0029] (2) inoculate the sunflower sunflower seeds after the disinfection in the step (1) in the seed germination medium, be 24 ℃, light intensity 2500lx in temperature, the constant temperature cultivation room that photoperiod is 16 / 10h (light / dark) carries out Germination induction, after 2 weeks of light cultivation, obtain a complete seed seedling plant with a height of 2.0 to 5.0 cm and 2 to 3 true leaves, su...

Embodiment 2

[0034] This embodiment tested different concentrations of 6-BA and GA 3 To the influence of the golden flower sunflower seed germination effect in the above-mentioned embodiment implementation process, concrete operation is as follows:

[0035] The sterilized goldenflower seeds were inoculated with different concentrations of 6-BA (0, 0.05, 0.1, 0.2, 0.5mg / L) and GA 3 (0, 0.02, 0.1, 0.2 mg / L) MS medium, in a constant temperature culture room with a temperature of 24° C., a light intensity of 2500 lx, and a light time of 16 h for seed germination induction. After 2 weeks of cultivation, the germination rate and growth of the seeds were counted. The results are shown in Table 1.

[0036] Table 1 Different concentrations of 6-BA and GA 3Effects on germination of sunflower seeds

[0037]

[0038] The results in Table 1 show that when 6-BA is added alone in the medium, as the concentration of 6-BA increases, the germination rate of the seeds first gradually increases and the...

Embodiment 3

[0040] The present embodiment has tested the influence of different concentrations of IBA and spermidine on the rooting effect of the adventitious buds of Anemone annuus, and the specific operations are as follows:

[0041] The elongated adventitious buds were transferred to 1 / 4MS medium supplemented with different concentrations of IBA (0.1, 0.2, 0.5, 1.0 mg / L) and spermidine (5.0, 10.0, 15.0, 20.0 mg / L) After 3 weeks of light cultivation, the rooting rate, the average number of roots produced by each explant and the average root length were counted. The results are shown in Table 2.

[0042] Table 2 Effects of different concentrations of IBA and spermidine on rooting effect of adventitious buds

[0043]

[0044] The results in Table 2 show that when IBA is added alone in the medium, as the concentration of IBA increases, the germination rate of the seeds first gradually increases and then decreases. Excellent, the rooting rate is 87.5%. Further studies have found that ...

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Abstract

The invention discloses a high-efficiency breeding method for sunflower plants. The mature and plump sunflower seeds are taken as initial materials, and the surface is sterilized; the sterilized seeds are inoculated into a germination medium, and cultivated under light to obtain aseptic seedlings ; Under aseptic conditions, the stem section of the aseptic seedling of goldenflower anemone is cut into sections, and inoculated in the adventitious bud induction medium. After light cultivation, adventitious bud clusters are induced at the incisions at both ends of the stem section; The stem section was transferred to the elongation medium for the elongation culture of adventitious buds; when the adventitious buds grew to complete leaves, they were transferred to the rooting medium for rooting culture, and after light culture, a test tube of goldenflower sunflower with strong root system was obtained Seedling. The breeding of sunflower seedlings can be carried out without the need for seedling breeding, thereby eliminating the time limit for raising sunflower seedlings, improving the seedling breeding coefficient, and laying the foundation for the large-scale breeding and variety improvement research of good golden sunflower lines in the later period. Base.

Description

technical field [0001] The invention relates to a method for propagating goldenflower sunflower, in particular to a method for efficiently breeding goldenflower sunflower plants. Background technique [0002] Golden flower sunflower (scientific name: Hibiseu manihot L.), also known as: vegetable hibiscus, also known as: wild hibiscus, is an annual herbaceous Malvaceae okra plant. In August 2003, the Chinese Academy of Agricultural Sciences discovered an endangered plant in Xingtai, Hebei. Among the more than 200 plants of the genus Okra, goldenflower has not only ornamental value, but also edible, medicinal and health care functions. It is also adaptable. Extremely strong, cold-resistant, heat-resistant, and salt-alkali-resistant, it can be planted in areas with high temperatures of 40°C and low temperatures of -10°C, and has extremely high development potential and utilization value. [0003] Sunflower can be planted in gardens, industrialized in gardens and fields, and ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 侯金艳吴丽芳
Owner 河南芙罗拉农业科技有限公司
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