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Multi-resistant high-yield alcohol yeast mutant strain TT31 and screening method thereof

A technology of alcoholic yeast and screening method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, etc. rate effect

Inactive Publication Date: 2011-08-17
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The strain used in this study has not yet been genetically characterized, and there are many difficulties in breeding directly by genetic engineering techniques while improving its tolerance and alcohol production

Method used

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  • Multi-resistant high-yield alcohol yeast mutant strain TT31 and screening method thereof
  • Multi-resistant high-yield alcohol yeast mutant strain TT31 and screening method thereof
  • Multi-resistant high-yield alcohol yeast mutant strain TT31 and screening method thereof

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Embodiment Construction

[0029] The present invention will be further described below in combination with specific embodiments. The specific embodiments should be understood as illustrative only and not restrictive. The following examples cannot be used to limit the protection scope of the present invention.

[0030] The technical route of the present invention is to perform ultraviolet mutagenesis on the starting strains, screen out the mutagenized strains that have a certain increase in tolerance and alcohol output during low pH fermentation, and then perform protoplast preparation and three rounds of genome reorganization on them, and go through TTC method one. Two-stage screening, two-stage screening by Dusley's tube fermentation, and three-stage screening by shaking flask fermentation, finally screened out yeast mutant strains with greater tolerance to acid, temperature and alcohol.

[0031] A multi-tolerant and high-yielding alcohol yeast mutant strain TT31, classified and named Saccharomyces crevisia...

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Abstract

The invention relates to a multi-resistant high-yield alcohol yeast mutant strain TT31 and a screening method thereof. A genome reorganizing technology is used for screening high temperature-resistant, alcohol-resistant and acidity-resistant high-yield alcohol yeast mutagenic strain. The method comprises the following steps of: mutating a three-strain yeast strain by ultraviolet; selecting a ten yeast mutant strain which have three resistance and are higher in alcohol yield by a triphenyltetrazolium chloride (TTC) method at a first-level screening, a dieulafoy pipe fermentation method at a second-level screening, and a bottle shaking fermentation method at a third-level screening; preparing protoplast of the ten yeast mutant strains; and reorganizing genome at thee turns to finally obtain the yeast mutant strain TT31 which has higher improvemrnt on temperature resistance, alcohol resistance and acidity resistance. Under the condition of 35 DEG C, the mutant strain is fermented for 72h with alcohol by taking sucrose as a carbon source, and the alcohol yield reaches 101.9g / L.

Description

Technical field [0001] The invention belongs to the field of microbial breeding, in particular to a multi-tolerant high-alcohol-yielding yeast mutant strain TT31 and a screening method thereof. Background technique [0002] Ethanol (commonly known as alcohol) is an important industrial raw material, which is widely used in the fields of chemical industry, food, beverage industry, military industry, daily chemical industry and medicine and health. In recent years, the excessive consumption of petroleum-based fuels as automobile fuels has caused serious environmental pollution. Ethanol is a clean and renewable energy source. Adding ethanol to gasoline can be used to replace or partially replace gasoline, which can not only reduce gasoline consumption, but also reduce environmental pollution. At present, the conversion of sugarcane, corn, cassava and fiber wastes into fuel ethanol has become an ideal way to solve the energy crisis, and the selection of excellent microbial strains i...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N13/00C12N15/04C12R1/865
Inventor 王昌禄潘静李风娟王玉荣陈勉华
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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