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Method for cultivating pearl by inducing triploid hyriopsis cumingii

A technology of triploids and triploids, applied in fish farming, application, climate change adaptation, etc., can solve problems such as differences in reproductive biology, fertilization and embryonic development between the three-sided sailfish and marine shellfish

Inactive Publication Date: 2011-01-12
ZHEJIANG SHANXIAHU PEARL GROUP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the unique mussel species in our country is also the main mussel species used for freshwater pearl culture to induce triploids of Trichophyllus sinensis. There are few related reports, only CN1957686A, which adopts the type of in vitro fertilization and embryonic development, and Its in vitro fertilization uses the eggs produced by the triploid S. sinensis and the sperm of the diploid S. sinensis, which itself needs the triploid S. sinensis as a parent.
[0006] Although the triangular sail clam and other marine molluscs are at the same level in biological classification and evolution, and have many similarities, there are many differences in reproductive biology between the triangular sail clam and marine molluscs, especially in the aspects of fertilization and embryonic development. big difference

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1 Selection and gender identification of parent mussels

[0046] Choose three-year-old Hyriopsis mussels with a shell length of 15cm or more, a shell thickness of 5cm or more, a complete shell, a strong clam body, a round abdomen, a strong adductor muscle, and no disease or injury, and then determine the male and female. Parent mussels are selected according to female: male=1:1.

[0047] 2 Cultivation of parent mussel

[0048] Separate the male and female clams from the parent clams from November to February of the following year, and hang them in the micro-flowing water of the pond. The water depth is about 1.5m. The water quality is required to be fresh, pollution-free, fat and thin, and the transparency is about 30cm. , Raise about 600 per mu. In the breeding season (early March), the female and male mussels are cultivated separately and strictly isolated.

[0049] 3 triploid induction

[0050] Begin by cultivating the female and male clams separately, and observe the sel...

Embodiment 2

[0135] 1 Selection and gender identification of parent mussels

[0136] Choose 4-year-old Hyriopsis mussels with a shell length of 15 cm or more, a shell thickness of 5 cm or more, a complete shell, a strong clam body, a round abdomen, strong adductor muscles, and no disease or injury, and then determine the male and female. The parent clams are selected according to female: male = 2:1.

[0137] 2 Cultivation of parent mussel

[0138] Separate the male and female clams from the parent clams from November to February of the following year, and hang them in the micro-flowing water of the pond. The water depth is about 1.5m. The water quality is required to be fresh, pollution-free, fat and thin, and the transparency is about 30cm. , Raise about 600 per mu. In the breeding season (early March), the female and male mussels are cultivated separately and strictly isolated.

[0139] 3 triploid induction

[0140] Observe the selected female mussels. If you find that the gills of the female...

Embodiment 3

[0166] 1 Selection and gender identification of parent mussels

[0167] Select 6-year-old Hyriopsis mussels with a shell length of 15 cm or more, a shell thickness of 5 cm or more, a complete shell, a strong clam body, a round abdomen, strong adductor muscle, and no disease or injury, and then determine the male and female. Parent mussels are selected according to female: male=3:1.

[0168] 2 Cultivation of parent mussel

[0169] Separate the male and female clams from the parent clams from November to February of the following year, and hang them in the micro-flowing water of the pond. The water depth is about 1.5m. The water quality is required to be fresh, pollution-free, fat and thin, and the transparency is about 30cm. , Raise about 600 per mu. In the breeding season (early March), the female and male mussels are cultivated separately and strictly isolated.

[0170] 3 triploid induction

[0171] Observe the selected female mussels. If you find that the gills of the female muss...

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Abstract

The invention discloses a method for cultivating a pearl by inducing triploid hyriopsis cumingii. The method comprises the following steps of: cultivating the triploid hyriopsis cumingii by induction, namely selecting diploid hyriopsis cumingii with age of 3 to 6, shell length of over 15cm and shell thickness of over 5cm in normal growth as parent clams; isolating the male clams from the female clams of the selected parent clams for cultivation respectively; tracking and observing the development condition of the eggs of the female clams; transferring the female clams into a pond for cultivating the male clams to perform natural insemination on the female clams after the eggs are maturely developed; observing the development condition of the fertilized eggs; putting the female clams into 300 to 450 mu mol / L aqueous solution of 6-dimethyl aminopurine after over 80 percent of the fertilized eggs have first polar bodies; and performing induction processing for 10 to 30 minutes to obtain induced female clams; cultivating the female clams, and hatching and raising the seedlings to obtain the triploid juvenile clams; and performing cultivation to obtain the triploid hyriopsis cumingii. In the method, the normal diploid hyriopsis cumingii is used as the parents, and the natural insemination is adopted, so that the induction success rate is improved to over 70 percent.

Description

Technical field [0001] The invention belongs to the technical field of pearl culture, and particularly relates to a method for inducing and cultivating triploid Hyriopsis mussels and cultivating freshwater pearls by using the cultivated triploid Hyriopsis mussels. Background technique [0002] Freshwater pearl cultivation has many years of history and cultivation experience in our country and has formed a scale, and it has an important position in freshwater cultivation. However, with the development of pearl culture industry and the continuous maturity of artificial propagation technology, the degradation and disease occurrence of pearl mussel germplasm resources caused by long-term multi-generation inbreeding have brought certain harm to the aquaculture industry. The degradation of the disease is concentrated in the premature sexual maturity, and the resulting slow growth and low disease resistance. The long pearl breeding cycle, serious diseases, low pearl quality and low eco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 弭忠祥陈海军韩继卫杨受保刘小锋刘文东
Owner ZHEJIANG SHANXIAHU PEARL GROUP CO LTD
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