Efficient tilia miqueliana strain culture method

A high-efficiency, linden plant technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of cumbersome domestication and transplanting of tissue cultured seedlings, low reproductive efficiency, low survival rate, etc., and achieve industrialized seedling cultivation , Simplify the breeding process and expand the breeding coefficient

Active Publication Date: 2018-03-06
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports on the tissue culture and breeding of Tilia Nanjing, but most of them use the stems of 1- to 3-year-old seedlings of Tilia Nanjing as materials. There is serious pollution in the breeding process, and the reproduction efficiency is low. Low survival rate and other issues make it difficult to meet the needs of Nanjing Tilia breeding

Method used

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  • Efficient tilia miqueliana strain culture method
  • Efficient tilia miqueliana strain culture method
  • Efficient tilia miqueliana strain culture method

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Embodiment 1

[0033] The method for the efficient breeding of the Nanjing linden strain of the present embodiment, the concrete steps are as follows:

[0034] (1) if figure 1 As shown, the current-year branches of the perennial Nanjing Tilia in the field were used as materials, and semi-lignified stems were cut as explants; after surface disinfection, cut into 1.0cm-sized sections with axillary buds, and inoculated on the primary culture medium , the germination induction of axillary buds was carried out in a constant temperature culture room with a temperature of 25°C, a light intensity of 2500lx, and a light cycle of 15h light / 9h dark; after 10 days of cultivation, the axillary buds germinated to about 1.0cm, as figure 2 As shown, the germination rate of axillary buds was 100%; wherein the primary medium was MS medium supplemented with 0.5mg / L TDZ, 0.25mg / L ZT, 30g / L sucrose and 7.0g / L agar.

[0035] (2) Clip the axillary buds in step (1), transfer them to the proliferation medium, and ...

Embodiment 2

[0041] The method for the efficient breeding of the Nanjing linden strain of the present embodiment, the concrete steps are as follows:

[0042] (1) Using the current-year branches of the perennial Nanjing Tilia in the wild as materials, cut semi-lignified stems as explants; after surface disinfection, cut into 1.0cm-sized sections with axillary buds, and inoculate them on the primary culture medium In the medium, the germination induction of axillary buds was carried out in a constant temperature culture room with a temperature of 26°C, a light intensity of 3000lx, and a light cycle of 14h light / 10h dark; after 8 days of cultivation, the axillary buds germinated to about 1.0cm, and the germination rate of axillary buds was 100%; The primary culture medium is MS medium supplemented with 1.0mg / LTDZ, 0.5mg / L ZT, 30g / L sucrose and 7.0g / L agar.

[0043] (2) Clip the axillary buds in step (1), transfer them to the proliferation medium, and carry out the proliferation and culture of t...

Embodiment 3

[0049] The method for the efficient breeding of the Nanjing linden strain of the present embodiment, the concrete steps are as follows:

[0050] (1) Using the current-year branches of the perennial Nanjing Tilia in the wild as materials, cut semi-lignified stems as explants; after surface disinfection, cut into 1.0cm-sized sections with axillary buds, and inoculate them on the primary culture medium The germination of axillary buds was induced in a constant temperature culture room with a temperature of 23°C, a light intensity of 2700lx, and a light cycle of 13h light / 11h dark; after 5 days of cultivation, the axillary buds germinated to about 1.0cm, and the germination rate of axillary buds was 100%; The primary medium is MS medium supplemented with 0.1mg / L TDZ, 0.05mg / L ZT, 30g / L sucrose and 7.0g / L agar.

[0051] (2) Clip the axillary buds in step (1), transfer them to the proliferation medium, and carry out the proliferation and culture of the axillary buds in a constant te...

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Abstract

The invention discloses an efficient tilia miqueliana strain culture method which comprises the following steps: cutting stem segments with axillary buds as explants, sterilizing surfaces, inoculatinginto a primary culture medium, and performing culture; cutting the axillary buds, inoculating into a proliferation medium, and inducing adventitious buds from the axillary buds; separating the adventitious buds into single buds, and inoculating into an elongation culture medium; moving the elongated adventitious buds outside a tissue culture chamber, and strengthening seedlings under a natural light condition; washing the elongated buds after seedling strengthening, cutting into appropriate micro cut segments, and performing rooting pretreatment; and performing planting fixation on morphological lower ends of the segments treated with rooting promotion liquid into a nutrient medium, and performing grown seedling culture on tilia miqueliana plants in a greenhouse. By adopting the method, the reproductive process, the rooting process and the strengthening process of tilia miqueliana elongated seedlings are combined, so that the proliferation coefficient of the tilia miqueliana is increased, the survival rate of tissue culture seedlings is increased, the seed seedling proliferation process is accelerated, and the industrial seedling culture of the tilia miqueliana is facilitated.

Description

technical field [0001] The invention relates to a rapid plant propagation technology, in particular to a method for efficient propagation of Nanjing Tilia strains. Background technique [0002] Nanjing Tilia (Tiliamiqueliana Maxim) belongs to Tiliaceae, Tilia is a deciduous tree, and it is a unique tree species in my country. Its tree shape is beautiful, majestic, full of yellow flowers in summer, fragrant flowers, less pests and diseases, strong resistance to smoke and harmful gases, it is an excellent garden ornamental tree species and honey plant; The bark can be used as medicine. It is an excellent tree species that integrates garden ornamental, nectar source plants, timber species and medicinal uses. Nanjing Tilia is produced in Jiangsu, Zhejiang, Anhui, Jiangxi and other provinces in my country. However, due to breeding difficulties and habitat destruction, the population and individual numbers of Nanjing Tilia have gradually declined, and even the wild resources are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯金艳吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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