33 results about "Tilia miqueliana" patented technology

The invention discloses a Tilia miqueliana seedling raising method, which includes seed collection, drying in the shade, soaking, multi-substrate variable temperature stratification and transplanting. Seedlings of Tilia miqueliana raised by the method break through dormancy in about 50 days, are high in survival rate and emergence percentage, and have developed roots.

The invention belongs to the technical field of plant seedling propagation and discloses a micropropagation method of tilia miqueliana. The method comprises steps as follows: A, culture of seedling of the tilia miqueliana; B, adventitious bud induction; C, high-voltage electrostatic field processing; D, subculture and rooting; E, acclimation and seedling hardening. A large quantity of tilia miqueliana explants easy to disinfect can be obtained, the success rate of primary culture is higher than 98%, the proliferation rate reaches 113 times, the survival rate reaches 95%, the problem of shortage of the tilia miqueliana in the market is effectively solved, and the application prospect is wide.

The invention relates to a tissue culture propagating method of Tilia miqueliana Maxim, which pertains to the technical field of ligneous plant plantlet vegetative propagation. The invention aims at solving the problem that both seed seedling raising (low seed germination rate and long dormancy stage) and cottage propagation of the Tilia miqueliana Maxim are difficult. The method comprises the following steps: the stem sections with axillary buds at the different ages are taken as explants which are respectively inoculated on inducing culture medium, the best explant is selected based on the bud inducing result; and the best disinfection method is selected by comparing the contamination rates of different disinfection ways; an optimal bud inducing culture medium and an optimal successive propagation culture medium are respectively screened by adopting an L9(3<3>) orthogonal experimental design; based on the successive culture medium, 12 culture media are prepared by adjusting the hormone levels; the best rooting sound plantlet hardening culture medium is screened according to rooting situation. By adopting the experiment, the best tissue culture propagating method of the Tilia miqueliana Maxim is obtained by optimization.

The invention provides a twig cutting method for tilia miqueliana. Twig cutting slips magnetic water treatment strength and frequency and external hormone treatment and cutting environmental conditions before cutting are strictly controlled, cutting rooting of tilia miqueliana can be achieved through steps of scion preparation, hormone treatment, magnetic water treatment, cutting and management after cutting, control over key links of twig cutting of tilia miqueliana is enhanced, magnetic treatment and external hormone act together to stimulate cutting slips to form callus tissue and root induction, the rooting environment is optimized, rapid rooting of the cutting slips can be guaranteed, meanwhile, rotting probability of the cutting slips can be reduced, the survival rate of twig cutting is above 85%, and good seedling basis can be built for seedling cultivation industrialization development of tilia miqueliana.

The invention discloses a method for increasing the wild Tilia miqueliana transplantation survival rate. The method includes the following steps of (1) seedling culture land consolidation, (2) seedling transplantation, (3) seedling treatment, (4) rooting accelerant irrigation and (5) seedling management. According to the method, the wild Tilia miqueliana transplantation survival rate reaches over 90.0% and the method has important significance in storage of Tilia miqueliana germplasm gene resources, prevention of loss of excellent Tilia miqueliana genetic genes and further research, development and utilization of wild Tilia miqueliana resources.

The invention relates to a propagation method for tilia miqueliana.The tilia miqueliana is bred through the steps of seed collection, seed storage, seed germination accelerating, cuttage breeding and transplanting.The probability of death of young tilia miqueliana saplings can be effectively reduced, and the effect of improving growth efficiency of the tilia miqueliana can be achieved.

The invention discloses a Tilia miqueliana rapid breeding method which comprises the following steps of: 1) selecting the half-lignified twig as an explant; 2) sterilizing the explant; and 3) soaking the sterilized explant in plant hormone for 2-12 hours, and then performing cuttage, wherein in the cuttage, the relative air humidity in a seedling raising room is required to be 80-95%, the temperature is controlled at 20-28 DEG C, and illumination lasts for 9-10 hours per day at the illumination intensity of 1,800-2,000 lux. The method disclosed by the invention can improve the survival rate of the Tilia miqueliana twig by cuttage propagation to over 50%, and also can improve the growth of the cutting.

The invention discloses a tilia miqueiana green branch cuttage method with nutrition pot substrates. The tilia miqueiana green branch cuttage method with nutrition pot substrates comprises the steps of placing nutrition pots loaded with mixed substrates of turf and garden soil in the volume ratio being (2:1) to (3:1) on a cuttage bed, and building a film covering arched shed and a shading shed frame; in the middle ten days of April to the last ten days of June, collecting annual tender twigs as cuttings, under moisture keeping and fresh keeping condition, performing cutting to obtain half-lignified cutting slips of which the length is 10-12cm, reserving one leaf at each top end, and performing cutting off treatment on basal parts; and performing macerating treatment on the cutting slips inrooting liquid, inserting the cutting slips after the macerating treatment in the mixed substrates of the nutrition pots, sequentially spraying mist for replenishing water, spraying sterilizing pesticides, and performing covering with films and shading nets. Through the adoption of the cuttage method provided by the invention, the highest cuttage rooting rate of tilia miqueiana green branches canreach 90%, and root systems of cuttage plantlets are developed; later-stage seedling hardening and transplanting links are simplified, operations are convenient, root systems are not damaged, and transplanting survival and seedling growth are facilitated; and the cuttage bed is arranged without limit by land parcel conditions, the production cost is low, and quick reproduction and production application of tilia miqueiana nursery stocks are facilitated.

The invention belongs to the technical field of propagation of plant seeds and seedlings, and discloses a tilia miqueliana layerage propagation method. The tilia miqueliana layerage propagation methodcomprises the following steps of A, performing female parent selection and culture; B, performing layerage selection and treatment; C, performing layerage substrate selection and treatment; D, executing a layerage method; and E, performing layerage seedling transplanting and fertilizing management. The tilia miqueliana layerage propagation method disclosed by the invention is simple to operate, tilia miqueliana seedlings can be cultivated in a large scale, massive female parents can be used for sprouting tillers and branches, and cost is reduced for seedling culture. The rooting time of layerage is 55d, the survival rate is 90% or above, the vacancy of tilia miqueliana market resources is effectively filled, and the application prospects are great.

The invention discloses a tilia miqueliana root cutting seedling growing method. Tilia miqueliana root cuttings are adopted for root cutting seedling growing, cottonseed hulls are used as seedling growing matrix, and the matrix is loosened and nutrient-rich and can promote germination of the tilia miqueliana rapidly. Through adopting electromagnetization water, penetration and dissolving capacity of the water can be reinforced. Through applying organic boron fertilizer in the matrix, rotten roots are controlled, sufficient fertilizer and excellent growing environment are provided, and growth and budding of the root cuttings are further promoted by plant growth hormone gibberellins. Roots of seedlings obtained by the seedling growing method are prevented from being injured when transplanted, survival rates of the seedlings are high, the tilia miqueliana can be planted in all seasons without being limited by climate, the seedlings of the tilia miqueliana come out evenly, and survival rate of afforestation is high.

The invention relates to drilling equipment, in particular to tilia miqueliana drilling equipment for agricultural production. The tilia miqueliana drilling equipment for agricultural production enables people to be easier. Tilia miqueliana drilling equipment for agricultural production comprises a bottom plate and brackets, wherein a servo motor is installed on the upper side of the bottom plate;the brackets and guide rods are fixedly connected to the two sides of the top of the bottom plate correspondingly, and the guide rods on the two sides are located between the brackets on the two sides. According to the tilia miqueliana drilling equipment, the tilia miqueliana can be fixed through a moving assembly, the tilia miqueliana can be drilled through a drilling assembly, manual operationis greatly reduced, and people can feel easier; the tilia miqueliana can be pushed to rotate by 45 degrees through a special-shaped rod, so that people do not need to manually rotate the tilia miqueliana, and the working efficiency can be improved; and a sliding frame can be attracted through magnets, so that a placement disc on the right side is not prone to rotating, and people can convenientlyplace the tilia miqueliana in the two placement discs.

The present invention mainly relates the technical field of food processing and discloses dendrobium huoshanense granules. The dendrobium huoshanense granules are prepared from the following raw materials: dendrobium huoshanense, tilia miqueliana flowers, bidens tripartita, stir-fried rice flour, an arthraxon hispidus herb extract, an abutilon indicum herb extract, pork blood, soybean oligosaccharides and lactic acid bacteria. Medical stone is added into the stir-fried rice flour, the materials are subjected to a primary high temperature fermentation, proliferation of the lactic acid bacteria is promoted, the other raw materials are added, mixture is subjected to a low temperature secondary fermentation and a long time soaking, fragrance is increased, taste is sour, sweet and palatable, leaching and merging of nutrients in the dendrobium huoshanense, tilia miqueliana flowers, bidens tripartita and medical stone are promoted, peculiar smell of the traditional Chinese medicines is removed, health-care effects are increased, nutrition is balanced and supplemented, organ vitality is enhanced, free radicals are eliminated, oxidation is prevented, cell malignant proliferation is inhibited, and effects of delaying aging and strengthening body are achieved; and after the fermentation of the raw materials, the raw materials are sterilized, the sterilized materials are freeze-dried, nutrients can be retained, the dendrobium huoshanense granules are easy for rehydration, during drinking, water at relatively low temperature can be added to conduct soaking during drinking, drinking time is saved, and the dendrobium huoshanense granules are in line with fast-paced lifestyle.

The invention belongs to the technical field of tree propagation, and particularly relates to a treatment method for increasing the germination rate of tilia miqueliana seeds. The treatment method comprises the following steps of 1, seed pretreatment; 2, seed disinfection treatment; 3, seed soaking treatment; 4, preparation of an induction culture medium; and 5, seed coat treatment. The seeds aresoaked in a gibberellic hormone solution, and then the soaked seeds are peeled, so that germination of the peeled tilia miqueliana seeds is effectively promoted, and the germination rate of the seedsis increased; and dormancy breaking and germination of the seeds are promoted through the gibberellic hormone solution, and the tilia miqueliana seeds which are soaked in the gibberellic hormone solution and peeled are cultured and germinated in the culture medium, so that mineral nutrition required by tilia miqueliana seed growth is guaranteed in the culture medium, seed growth is promoted to behealthy and strong, resistance is enhanced, seed vigor is improved, the germination rate of the tilia miqueliana seeds can reach 80% or above, the germination rate is high, batch propagation can be achieved after management, and planting and popularization are facilitated.

The invention belongs to the field of plant cultivation, and relates to a tissue culture seedling raising method, in particular to a rooting and seedling raising method for tilia miqueliana. The rooting and seedling raising method for tilia miqueliana comprises the following steps that (1), a sterile explant is prepared; (2), sterile seedlings are prepared; (3), tissue culture seedlings are prepared; (4), rooting seedling tissue culture seedlings are prepared; and (5), seedling hardening is carried out. According to the rooting and seedling raising method for tilia miqueliana, the culture period is short, and the total period of proliferation and rooting is about 10 weeks; the multiplication coefficient is high and can reach 1 : (4-4.5), the plant height after multiplication is about 3.5 cm, and the survival percent of pass after multiplication is greater than 98%; the rooting rate is 100%, and the survival rate after rooting is greater than 99%; and the survival rate after transplanting is greater than 98%, and the rooting and seedling raising method is suitable for industrialized seedling production of tilia miqueliana.

The invention discloses a device for removing tilia miqueliana shells and separating seeds by liquid nitrogen and an operation method of the device, belongs to the technical field of tilia miqueliana planting, and adopts an integrated design, so that treatment of the tilia miqueliana shells and seeds is completed in the device, and the device comprises a treatment container, a waste shell collecting barrel and a shade drying platform, wherein the treatment container is used for removing shells of tilia miqueliana and washing the tilia miqueliana with water to separate seeds, the waste shell collecting barrel is used for collecting the removed shells of tilia miqueliana, and the shade-drying platform is used for carrying out shade-drying treatment on separated kernels of tilia miqueliana; then liquid nitrogen enters the treatment container in an injection mode, cell structures of the shells are destroyed through the liquid nitrogen quick-freezing and ultralow-temperature freezing and thawing effects, the biological mechanical force at the joints of the shells and the core and the skin is reduced, and therefore the purpose of quickly removing the shells is achieved, and complete kernels in the shells cannot be damaged through ultralow-temperature treatment within a short time; and the seed kernels can hold a high plumpness rate.

The invention discloses a real-time quantitative PCR (Polymerase Chain Reaction) internal reference group gene of tilia miqueliana under different tissues and stress treatment conditions as well as a screening method and application thereof, and belongs to the technical field of plant genetic engineering. The 15 reference genes disclosed by the invention are derived from full-length transcriptome sequences of different tissues of tilia miqueliana, 15 pairs of specific real-time quantitative PCR (Polymerase Chain Reaction) primers are designed according to sequence information, and real-time quantitative PCR amplification is respectively carried out on different tissues of tilia miqueliana and plant materials subjected to stress treatment to obtain Ct values of the reference genes; and sorting the stability of the candidate reference genes from strong to weak, finally comprehensively evaluating the sorting result, and screening to obtain the most stable reference genes and combinations under different tissue and stress treatment conditions. The reference gene obtained through screening and the primer thereof have the advantages of being high in specificity and wide in applicability, and the blank of tilia miqueliana reference gene research is filled.

The invention belongs to the technical field of tree propagation, and in particular relates to a root cuttage seedling-raising method of tilia miqueliana. The root cuttage seedling-raising method of tilia miqueliana comprises the following steps of: (1), performing stump preparation; (2), performing stump processing; (3), processing before cuttage; (4), performing hormone processing; (5), performing cuttage processing; and (6), managing after cuttage. According to the root cuttage seedling-raising method of tilia miqueliana in the invention, the temperature, the humidity, the illumination andthe ventilation in a culture room are effectively controlled; a good growth environment is provided for stumps; plant hormone solution and auxin are adopted; naphthylacetic acid is a broad-spectrum plant growth regulator; therefore, cell division and expansion can be promoted; tillering can be increased; the percentage of earbearing tiller and the thousand seed weight can be increased; cuttage branches can be promoted to root; the survival rate of seedlings is high; the auxin can promote cell division and differentiation, promote synthesis of RNA and protein, and stimulate growth of leaves andbuds; compound waste liquid is used for promoting rooting of stumps and preventing water deficit or decay of the stumps; and thus, the survival rate of the stumps is increased.

The invention discloses a tilia miqueliana tissue culture method, including the steps of selecting explants, to be specific, taking a strong shoot on the southeast branch of two-year-old tilia miqueliana as the first explant, and taking a shoot on the northwest semi-ligneous branch of one-year-old tilia miqueliana tissue culture as the second explant; preprocessing; performing cuttage grafting byparticularly grafting the second explant to the lateral side of the first explant in regular manners, fastening the grafted portions with linen thread, and cutting 2-3 wedged cuts on the circumferenceof the first explant; and finally, cultivating. The method has the advantages that the plant of tilia miqueliana cultured by the method has high adaptability to territory, and is easy to be cared during growth.