Tilia miqueliana rapid breeding method
A fast, explanted technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low survival rate of cuttings, small amount of seedling mother trees, uneven emergence of seedlings, etc., so as to improve the survival rate , Improve the effect of growth
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Embodiment 1
[0014] Embodiment 1: a kind of Nanjing linden rapid breeding method, at first select semi-lignified twigs as explants, then explants are sterilized, and then the explants after the sterilized treatment are soaked in phytohormone for 2 hours and then carried out For cutting, when cutting, the relative air humidity in the seedling room should be 80-95%, the temperature should be controlled at 20-28°C, and the light should be 9 hours / day, and the light intensity should be 1800-2000 lux. The phytohormone is prepared by adding water to the following components: 40 mg of ABT1, 1 mg of gibberellin, 20 g of disodium edetate, 0.2 mg of potassium iodide, 50 mg of ammonium nitrate, and 1 mg of boric acid. When preparing the above-mentioned phytohormones: first, add ABT1, gibberellin, disodium edetate, potassium iodide, ammonium nitrate, and boric acid to 1 liter with water, and then adjust the pH value to 5.5.
[0015] The method for disinfecting the explants in the above method is as fo...
Embodiment 2
[0017] Embodiment 2: a kind of Nanjing Tilia rapid breeding method, at first select semi-lignified twigs as explants, then explants are sterilized, and then the explants after the sterilized treatment are soaked in phytohormone for 12 hours and then carried out For cutting, the relative humidity of the air in the seedling cultivation room should be 80-95%, the temperature should be controlled at 20-28°C, and the light should be 10 hours / day, and the light intensity should be 1800-2000 lux. The phytohormone is prepared by adding water to the following components: 60 mg of ABT1, 2 mg of gibberellin, 30 g of disodium edetate, 0.5 mg of potassium iodide, 60 mg of ammonium nitrate, and 3 mg of boric acid. When preparing the above-mentioned phytohormones: first, add water to 1 liter of ABT1, gibberellin, disodium edetate, potassium iodide, ammonium nitrate, and boric acid, and then adjust the pH value to 6.5.
[0018] The method for disinfecting the explants in the above method is a...
Embodiment 3
[0020] Embodiment 3: a kind of Nanjing Tilia rapid breeding method, at first select semi-lignified twigs as explants, then explants are disinfected, and then the explants after the disinfection process are soaked in phytohormone for 8 hours and then carried out For cutting, the relative humidity of the air in the seedling cultivation room should be 80-95%, the temperature should be controlled at 20-28°C, and the light should be 9 hours / day, and the light intensity should be 1800-2000 lux. The phytohormone is prepared by adding water to the following components: 50 mg of ABT1, 1 mg of gibberellin, 25 g of disodium edetate, 0.3 mg of potassium iodide, 55 mg of ammonium nitrate, and 2 mg of boric acid. When preparing the above-mentioned phytohormones: first, add water to 1 liter of ABT1, gibberellin, disodium edetate, potassium iodide, ammonium nitrate, and boric acid, and then adjust the pH value to 6.
[0021] The method for disinfecting the explants in the above method is: fir...
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