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Transcriptome-based Tilia est-ssr primers and their screening methods and applications

A Tilia genus, transcriptome technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., can solve the problem of lack of efficient nuclear gene SSR markers

Active Publication Date: 2020-10-23
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology

For a long time, efficient, reliable, stable and co-dominant nuclear gene SSR markers have been lacking in Tilia plants

Method used

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  • Transcriptome-based Tilia est-ssr primers and their screening methods and applications
  • Transcriptome-based Tilia est-ssr primers and their screening methods and applications
  • Transcriptome-based Tilia est-ssr primers and their screening methods and applications

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with specific embodiments, and the given examples are only for clarifying the present invention, not for limiting the scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0022] The present invention provides full-length transcriptome sequencing, combined with bioinformatics methods for SSR sequence search and SSR marker primer design and verification, and its specific implementation is as follows:

[0023] 1. Assembly and screening of transcriptome data

[0024] 1.1 RNA extraction and detection

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Abstract

The invention discloses a set of EST-SSR molecular markers developed based on a full-length transcriptome sequence of tilia miqueliana and relates to 27 pairs of primers in all with nucleic acid sequences shown in SEQ ID No. 1-54 of a sequence listing. The 27 pairs of EST-SSR markers disclosed by the invention are SSR amplification primers obtained through screening candidate SSR loci based on thetranscriptome sequence and carrying out designing, are verified as definite SSR markers through taking different species of this tilia as materials and carrying out amplification by selecting genomicDNA and have the advantages of rich polymorphism, stability in amplification, good repeatability, codominance, easiness in detection and the like. This group of markers fill up a blank in codominantmarkers of the tilia, belong to the fields of variety identification, genetic pedigree analysis, germ plasm resource protection, assisted breeding and the like of plants of this tilia and can be usedfor greatly promoting researches and development application of tilia resources.

Description

technical field [0001] The invention relates to a transcriptome-based Tilia EST-SSR primer and a screening method and application thereof, belonging to the technical field of molecular marker technology development and application. Background technique [0002] Tilia L., a deciduous tree, is a genus with special morphology and the only northern hemisphere distribution in Malvaceae. It is one of the characteristic and dominant genus plants in deciduous to evergreen broad-leaved forests in the northern temperate zone. Species Diversity Center located in our country. The plants of this genus have a very long history of cultivation in the world, and have the functions of wood, fiber, medicine, landscape and honey source, etc., and the development and application prospects are very broad. [0003] Tilia plants have obvious characteristics, and the inflorescences and infructomes have large bracts with unique leaf shapes, which are easy to distinguish from other groups. The lates...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 王欢利汤诗杰王仲伟黄犀朱珣之罗会婷严灵君
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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