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A micro-propagation and seed technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of increasing the difficulty of pollution control, unsatisfactory effects, and serious vitrification.
Active Publication Date: 2015-12-30
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology
However, in the stage of factory production, the effect is still not satisfactory, and the breeding base is the key to the scale of factory production
However, the pollution control of the first generation of woody plants is extremely related to the explant material. The development and aging of the explant will increase the difficulty of pollution control. If the explant is too young, vitrification will be extremely serious.
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Embodiment 1
[0059] A micropropagation method of Nanjing Tilia, which comprises the steps of:
[0060] 1) selection of female parent material: select 50 annual seedlings as the female parent of the micropropagation explant;
[0061] The method of cultivating annual seedlings is to collect seeds in the previous year before October 15th, sow them as they are harvested, and use the sand storage method. After the seeds germinate, they are transplanted into containers for cultivation;
[0062] 2) Cultivation environment: Greenhouse cultivation is adopted, the light intensity range is 20000-30000lx, and the temperature range is 12-36°C;
[0063] 3) Moisture control: leaf surface humidity is controlled at 20%-50%, soil humidity is controlled at 40%-90%;
[0064] 4) Cultivation method: use container cultivation, the container is 50cm away from the ground, and 1 / 4 Hoagland's nutrient solution is applied to keep the diameter of the plant tender green, and the axillary buds do not form scales.
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Abstract
The invention belongs to the technical field of plant seedling propagation and discloses a micropropagation method of tilia miqueliana. The method comprises steps as follows: A, culture of seedling of the tilia miqueliana; B, adventitious bud induction; C, high-voltage electrostatic field processing; D, subculture and rooting; E, acclimation and seedling hardening. A large quantity of tilia miqueliana explants easy to disinfect can be obtained, the success rate of primary culture is higher than 98%, the proliferation rate reaches 113 times, the survival rate reaches 95%, the problem of shortage of the tilia miqueliana in the market is effectively solved, and the application prospect is wide.
Description
technical field [0001] The invention belongs to the technical field of plant seedling propagation, and in particular relates to a micropropagation method of Nanjing Tilia. Background technique [0002] Nanjing Linden, tree, 20 meters high, bark off-white; shoots with yellow-brown hairs, terminal buds ovate, covered with yellow-brown hairs. Leaves are oval, 9-12 cm long, 7-9.5 cm wide, apex acutely mucronate, base heart-shaped, straight or slightly oblique, upper glabrous, lower gray or gray-yellow stellate hairs, lateral veins 6 -8 pairs, with neatly serrated margins; petioles 3-4 cm long, cylindrical, tomentose. The cymes are 6-8 cm long, with 3-12 flowers, and the peduncle is gray hairy; the flower stalk is 8-12 mm long; the bracts are narrow and oblanceolate, 8-12 cm long, 1.5-2.5 cm wide, Stellate pilose on both sides, initially dense, apex obtuse, base narrow, lower part 4-6 cm connate with inflorescence stalk, short stalk, stalk 2-3 mm long, sometimes sessile; sepals...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 汤诗杰李乃伟束晓春王中伟王欢利
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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