Method of propagation of Tilia miqueliana by tissue culture

A technique of linden tissue and culture medium, which is applied in the field of woody plant seedling propagation

Inactive Publication Date: 2009-01-21
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has not been any report on the tissue culture research of Nanjing Tilia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. On April 15, 2006, 50 robust annual seedlings of Nanjing Tilia were selected. Cut the aerial parts, remove the leaves, and use the stem segments as explants, each stem segment has an axillary bud, 2 cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaker for 15-20 minutes. After rinsing with running water for 20-30 minutes, sterilize with 75% ethanol for 50-60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash 4 times with sterile water, shaking for 3-5 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.2mg / L+KT0.05mg / L+IBA0.02mg / L+GA 3 0.1mg / L, add agar 6.5g / l, sucrose 30g / l. The medium pH is 5.8-6.0. Each bottle received 3 plants, a total of 70 bottles were connected. Under the conditions of temperature 20-26°C, light time 10h / day, and light inten...

Embodiment 2

[0018] 1. On April 25, 2007, 30 robust annual seedlings of Nanjing Tilia were selected. Cut the aerial parts, remove the leaves, and use the stem segments as explants, each stem segment has an axillary bud, 2 cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaker for 15-20 minutes. After rinsing with running water for 20-30 minutes, sterilize with 75% ethanol for 50-60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash 4 times with sterile water, shaking for 3-5 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.2mg / L+KT0.05mg / L+IBA0.02mg / L+GA 3 0.1mg / L, add agar 6.5g / l, sucrose 30g / l. The medium pH is 5.8-6.0. Each bottle received 3 plants, a total of 50 bottles were connected. Under the conditions of temperature 20-26°C, light time 10h / day, and light inten...

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Abstract

The invention relates to a tissue culture propagating method of Tilia miqueliana Maxim, which pertains to the technical field of ligneous plant plantlet vegetative propagation. The invention aims at solving the problem that both seed seedling raising (low seed germination rate and long dormancy stage) and cottage propagation of the Tilia miqueliana Maxim are difficult. The method comprises the following steps: the stem sections with axillary buds at the different ages are taken as explants which are respectively inoculated on inducing culture medium, the best explant is selected based on the bud inducing result; and the best disinfection method is selected by comparing the contamination rates of different disinfection ways; an optimal bud inducing culture medium and an optimal successive propagation culture medium are respectively screened by adopting an L9(3<3>) orthogonal experimental design; based on the successive culture medium, 12 culture media are prepared by adjusting the hormone levels; the best rooting sound plantlet hardening culture medium is screened according to rooting situation. By adopting the experiment, the best tissue culture propagating method of the Tilia miqueliana Maxim is obtained by optimization.

Description

1. Technical field [0001] The invention relates to a Nanjing Tilia tissue culture propagation method, which belongs to the technical field of woody plant seedling propagation. 2. Background technology [0002] Nanjing Tilia (Tilia miqueliana Maxim) belongs to Tiliaceae, Tilia (Tilia) deciduous trees. It is an excellent garden ornamental tree species, which can be used as a street tree, shade tree, backbone tree species for community greening, etc. At present, the wild population of Nanjing Tilia is very small, and the natural regeneration ability is poor, and the population is declining in the community. Relevant studies have shown that the seed propagation and cutting propagation of Nanjing Tilia are very difficult, so the resources of Nanjing Tilia are very scarce. [0003] The research on tissue culture of woody plants started in the early 1950s and did not develop significantly until the late 1970s. At present, according to incomplete statistics, there are more than 9...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 汤诗杰李乃伟郑贵鸣郭忠仁陆小青
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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