Tissue culture method for increasing multiplication coefficient of catalpa bungei

A technology of proliferation coefficient and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of low value-added coefficient of tissue culture seedlings

Inactive Publication Date: 2014-09-24
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the results of this experiment, it can be found that the value-added coefficient of the tissue-cultured seedlings of Catalpa is on the low side. In order to promote the large-scale promotion of Catalpa, the value-added coefficient of the tissue-cultured seedlings must be increased.

Method used

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  • Tissue culture method for increasing multiplication coefficient of catalpa bungei
  • Tissue culture method for increasing multiplication coefficient of catalpa bungei
  • Tissue culture method for increasing multiplication coefficient of catalpa bungei

Examples

Experimental program
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Effect test

Embodiment Construction

[0017] (1) Establishment of sterile culture

[0018] Select the young shoots of catalpa trees in the current year, cut off the leaves and petioles, cut into 1.0-1.5cm long stems with axillary buds, soak the stems with buds in the supernatant of washing powder for 30 minutes, and scrub the segments with a soft brush Then rinse under running water for 30 minutes; soak in 75% ethanol for 30 seconds on the ultra-clean workbench, rinse with sterile water twice, then treat with 0.1% mercury chloride for 8 minutes, and rinse with sterile water for 6 times to obtain Bacterial explants.

[0019] (2) Induction of sprouts

[0020] Inoculate the obtained sterile stem section with buds on the induction medium to promote the germination and growth of axillary buds. The induction medium is MS basic medium, add 0.05~0.3mg / L of IBA, 0.2~1.0mg / L of BA, And the sucrose of 30g / L and the agar of 7g / L, adjust medium pH to be 6.0. The design of medium hormone combination and its corresponding axi...

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Abstract

The invention discloses a tissue culture method for increasing the multiplication coefficient of catalpa bungei. The tissue culture method comprises five steps of building sterile culture, inducing axillary bud, performing multiplication of the axillary bud, performing rooting culture of bud seedling and performing tissue culture of seedling. The method is used for culturing tissue and quickly culturing seedling of the catalpa bungei; the method has the advantages of high bud induction rate, large multiplication coefficient, high rooting rate and high transplanting survival rate. By virtue of the method, an optimal culture medium formula of the catalpa bungei is selected and prepared by 1.5mg / L of MS+BA, 0.1mg / L of ZT and 0.2mg / L of IAA, and a subculture method for subculturing stem and root callus during subculturing is also selected; the multiplication coefficient of catalpa bungei is 11.0-23.2, and the average multiplication coefficient is 15.3. By virtue of the method, a seedling culturing method good in multiplication speed, short in production cycle and good in stability is provided for large-scale cultivation of the catalpa bungei in the forestry production.

Description

technical field [0001] The invention relates to a tissue culture method for improving the multiplication coefficient of catalpa. Background technique [0002] Catalpa bungei is a tall tree of the Bignoniaceae genus Catalpa. It is native to China and has a cultivation history of more than two thousand years. The catalpa tree is tall and straight, with a dense crown. The branches, leaves and flowers all have high ornamental value. Among China's rich tree resources, only its "wood" has both appearance and appearance, and has been known as the "King of Wood" since ancient times. Catalpa has strong adaptability and is an excellent and precious timber tree species that can be cultivated in large areas. 'Yu No. 1' has smooth bark without cracking, straight and round trunk, and a long flowering period. In addition to the characteristics of traditional catalpa, it also has strong fast-growing properties. It is a rare and improved species of catalpa. [0003] There are many reports...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王鹏杨如同李亚马玲玲汪庆李林芳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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