A kind of tissue culture method that improves catalpa multiplication coefficient

A tissue culture, multiplication coefficient technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of low value-added coefficient of tissue culture seedlings

Inactive Publication Date: 2016-07-06
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

From the results of this experiment, it can be found that the value-added coefficient of the tissue-cultured seedlings of Catalpa is on the low side. In order to promote the large-scale promotion of Catalpa, the value-added coefficient of the tissue-cultured seedlings must be increased.

Method used

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  • A kind of tissue culture method that improves catalpa multiplication coefficient
  • A kind of tissue culture method that improves catalpa multiplication coefficient
  • A kind of tissue culture method that improves catalpa multiplication coefficient

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Experimental program
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Embodiment Construction

[0017] (1) Establishment of sterile culture

[0018] Select the young shoots of catalpa trees in the current year, cut off the leaves and petioles, cut into 1.0-1.5cm long stems with axillary buds, soak the stems with buds in the supernatant of washing powder for 30 minutes, and scrub the segments with a soft brush Then rinse under running water for 30 minutes; soak in 75% ethanol for 30 seconds on the ultra-clean workbench, rinse with sterile water twice, then treat with 0.1% mercury chloride for 8 minutes, and rinse with sterile water for 6 times to obtain Bacterial explants.

[0019] (2) Induction of sprouts

[0020] Inoculate the obtained sterile stem section with buds on the induction medium to promote the germination and growth of axillary buds. The induction medium is MS basic medium, add 0.05~0.3mg / L of IBA, 0.2~1.0mg / L of BA, And the sucrose of 30g / L and the agar of 7g / L, adjust medium pH to be 6.0. The combination design of medium hormone and its corresponding axi...

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Abstract

The invention discloses a tissue culture method for improving the multiplication coefficient of catalpa, which includes five technical steps of establishment of sterile culture, induction of axillary buds, multiplication of axillary buds, rooting culture of sprouts and cultivation of tissue culture seedlings. Utilizing this method to carry out catalpa tissue culture rapid seedling cultivation has the advantages of high bud induction rate, large multiplication coefficient, high rooting rate and high transplant survival rate, especially in the present invention, the optimum medium formula MS+BA1 for catalpa multiplication is selected .5mg / L+ZT0.1mg / L+IAA0.2mg / L, and the subculture method together with the callus at the base of the stem section during subculture, the value-added coefficient of catalpa is 11.0-23.2, and the average multiplication coefficient reaches 15.3 . The method can provide a seedling raising method with fast propagation speed, short production cycle and good stability for large-scale cultivation of catalpa in forestry production.

Description

technical field [0001] The invention relates to a tissue culture method for improving the multiplication coefficient of catalpa. Background technique [0002] Catalpa (Catalpabungei) is a tall tree of the Bignoniaceae (Bignoniaceae) genus Catalpa. It is native to China and has a cultivation history of more than two thousand years. The catalpa tree is tall and straight, with a dense crown. The branches, leaves and flowers all have high ornamental value. Among China's rich tree resources, only its "wood" has both appearance and appearance, and has been known as the "King of Wood" since ancient times. Catalpa has strong adaptability and is an excellent and precious timber tree species that can be cultivated in large areas. 'Yu No. 1' has smooth bark without cracking, straight and round trunk, and a long flowering period. In addition to the characteristics of traditional catalpa, it also has strong fast-growing properties. It is a rare and improved species of catalpa. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王鹏杨如同李亚马玲玲汪庆李林芳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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