The invention discloses an efficient 
tilia miqueliana strain culture method which comprises the following steps: 
cutting stem segments with axillary buds as explants, sterilizing surfaces, inoculatinginto a 
primary culture medium, and performing culture; 
cutting the axillary buds, inoculating into a proliferation medium, and inducing adventitious buds from the axillary buds; separating the adventitious buds into single buds, and inoculating into an elongation culture medium; moving the elongated adventitious buds outside a 
tissue culture chamber, and strengthening seedlings under a natural light condition; washing the elongated buds after 
seedling strengthening, 
cutting into appropriate micro 
cut segments, and performing rooting pretreatment; and performing planting fixation on morphological lower ends of the segments treated with rooting promotion liquid into a 
nutrient medium, and performing grown 
seedling culture on 
tilia miqueliana plants in a 
greenhouse. By adopting the method, the 
reproductive process, the rooting process and the strengthening process of 
tilia miqueliana elongated seedlings are combined, so that the proliferation coefficient of the 
tilia miqueliana is increased, the 
survival rate of 
tissue culture seedlings is increased, the seed 
seedling proliferation process is accelerated, and the industrial seedling culture of the 
tilia miqueliana is facilitated.