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37results about How to "Save training space" patented technology

Detoxification and cell pan rapid propagation method for purple sweet potatoes

The invention discloses a detoxification and cell pan rapid propagation method for purple sweet potatoes and belongs to the technical field of crop variety breeding. The method comprises the following steps: disinfecting stem tips of the purple sweet potatoes; peeling and inoculating meristems of the stem tips of the purple sweet potatoes; culturing the meristems of the stem tips of the purple sweet potatoes; selecting the cell pan and a substrate; transplanting and rapidly propagating a virus-free tube seedling of the purple sweet potatoes and the like. According to the method disclosed by the invention, the formula of a detoxification and regeneration culture medium of purple sweet potatoes is improved, and the method is convenient to operate and great in inoculum size. By using the cell pan, the rapid propagation speed of a virus-free seedling is greatly accelerated. Furthermore, the method is high in propagation coefficient and is not limited by seasons.
Owner:JIANGSU ACAD OF AGRI SCI

Method for rooting of horseradish outside tissue culture seedling bottle

The invention discloses a method for rooting of horseradish outside a tissue culture seedling bottle. The method is characterized by comprising the following steps of: utilizing tissue culture to induce the stem and the tip of the horseradish to obtain a rootless bud in a test tube; after subculture, domesticating the bud and cutting the bud to obtain a simple bud; inserting the bud into a soil matrix; and applying root promoter and 1 / 2 MS nutrient solution to the bud each week. After being inserted in the soil matrix for 30 days, the bud can have a good rooting effect. The technology greatly shortens the production cycle of tissue culture seedlings of the horseradish and reduces the production cost.
Owner:JINGGANGSHAN UNIVERSITY

Method for producing inactivated vaccine of H9N2 subtype of avian influenza virus and product of inactivated vaccine

The invention discloses a method for producing an inactivated vaccine of H9N2 subtype of avian influenza virus and a product of the inactivated vaccine, and belongs to the field of production and application of inactivated vaccines of H9N2 subtype of avian influenza virus. The method comprises the following steps: (1) culturing a MDCK cell by utilizing a cell factory; (2) inoculating H9N2 subtype of avian influenza virus, and proliferating virus in the cell factory, wherein the microbial preservation number of the H9N2 subtype of avian influenza virus is CGMCC No.9325; and (3) harvesting the virus, and preparing the inactivated vaccine. Immune protection tests prove that the protective rate of the H9N2 subtype of avian influenza virus produced by the method is 100 percent, and the antibody level of a cytotoxicity immunity group at each point in time is higher than that of a germ toxicity immunity group, and rises relatively high. Virus liquid produced by the method can be used for preparing medicines or reagents for preventing or treating H9N2 subtype of avian influenza virus.
Owner:JIANGSU ACAD OF AGRI SCI

Hydroponic planting device and method for planting plants by using same

The invention discloses a hydroponic planting device, which comprises a cultivation tank and a cover plate, wherein the bottom of the cultivation tank is rectangular; the cover plate is larger than the bottom plate of the cultivation tank; and twelve small holes used for planting plants and one large hole used for placing an aeration device are designed on the front side of the cover plate. The invention further discloses a method for water cultivation of plants by using the hydroponic planting device. The invention has the advantages that the hydroponic planting device provides a convenient and practical device for rapid growth of plants in an optimal manner in a stable water environment, is suitable for long-term cultivation of plants such as flowers and the like, is suitable for cultivation of a large amount of plant seedlings in a short term in a laboratory, and can not only save a nutrient solution, but also reduce the cultivation space occupied by a container.
Owner:DASHUN INT FLOWER

Gamification teaching platform and method

The invention discloses a gamification teaching platform and method and relates to the technical field of electronic teaching. Through an electronic game mode, working scenes, working actions, working relations and assessment evaluation required by engineering and integration courses are simulated. Defects of limited field equipment, incapability of ensuring the participation rate of students, lack of interestingness of practical training tasks, loss of objectivity in assessment and evaluation links and the like of engineering and integration courses at the present stage can be made up. The platform comprises a server, a teacher end and a student end, wherein the teacher end and the student end are in communication connection with the server to realize teaching information exchange; the server comprises a game task management module, a display module, a help seeking and rescuing module, an evaluation module, an award module, an attendance checking module and a user information management module; a teacher can randomly select scripts in the game task management module to generate different game tasks; the teacher end comprises a game task monitoring module; the student end comprises a game task implementation module.
Owner:广东省交通运输高级技工学校

Sports dancing barre suitable for any site

The invention discloses a sports dancing barre suitable for any site. The sports dancing barre comprises a support rod, wherein the top of the support rod is provided with a cross rod; the support rod is sheathed with a casing pipe; the top end of the support rod is provided with a connection plug; the casing pipe is provided with a regulation screw; the casing pipe is connected with one end of a fixed rod through a spindle; the bottom end of the casing pipe is provided with a connection swivel pin; the connection swivel pin is connected with a fixed lug; the fixed lug is arranged on the fixed pin of the casing pipe; the surface of the fixed pin of the casing pipe is provided with a minitype operation touch screen and a USB (Universal Serial BUs) charging interface; a music player and a storage battery are arranged in the fixed pin of the casing pipe; one side of the fixed pin of the casing pipe is provided with an angle regulation motor; the angle regulation motor is connected with the fixed pin of the casing pipe through a flexible connecting rod. The sports dancing barre does not need to be installed on a wall surface so as to greatly save a training site and increase the learning of students, unnecessary resource waste is not caused, the sports dancing barre is convenient for carrying and is reasonable in design, and practicality is improved.
Owner:陈利峰

Virtual automobile drive visual system based on Open ground automobile system (GVS)

The invention discloses a virtual automobile drive visual system base on Open ground automobile system (GVS). The virtual automobile drive visual system based on the Open GVS comprises a trainee machine module, a trainer machine module and a communication module. The trainee machine module comprises a first input module (1), a first display module (2), a second display module (3), a third display module (4), a first model storage module (5) and a first main processing module (6). The trainer machine comprises a second input module (7), a second main processing module (8),a fourth display module (9) and a second model storage module (10). The virtual automobile drive visual system based on the Open GVS provides an automobile drive simulated training environment with high simulation degree for trainees, and trainers can conduct examination and checking on learning and operating training conditions of the trainees through a trainer machine. The virtual automobile drive visual system based on the Open GVS has the advantages of being economic, safe, high in fidelity and the like.
Owner:NANJING UNIV OF SCI & TECH

Efficient tilia miqueliana strain culture method

The invention discloses an efficient tilia miqueliana strain culture method which comprises the following steps: cutting stem segments with axillary buds as explants, sterilizing surfaces, inoculatinginto a primary culture medium, and performing culture; cutting the axillary buds, inoculating into a proliferation medium, and inducing adventitious buds from the axillary buds; separating the adventitious buds into single buds, and inoculating into an elongation culture medium; moving the elongated adventitious buds outside a tissue culture chamber, and strengthening seedlings under a natural light condition; washing the elongated buds after seedling strengthening, cutting into appropriate micro cut segments, and performing rooting pretreatment; and performing planting fixation on morphological lower ends of the segments treated with rooting promotion liquid into a nutrient medium, and performing grown seedling culture on tilia miqueliana plants in a greenhouse. By adopting the method, the reproductive process, the rooting process and the strengthening process of tilia miqueliana elongated seedlings are combined, so that the proliferation coefficient of the tilia miqueliana is increased, the survival rate of tissue culture seedlings is increased, the seed seedling proliferation process is accelerated, and the industrial seedling culture of the tilia miqueliana is facilitated.
Owner:HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI

Cell culture chamber for collecting radioactive gases

The invention discloses a cell culture chamber for collecting radioactive gases, which comprises a culture dish base, a screw cap and a non-screw cap, wherein the culture dish base comprises a small dish and a big dish; the edge of the big dish is provided with a thread structure; the bottom surfaces of the small dish and the big dish are concentric and fixed together; the screw cap can be screwed on the big dish; the non-screw cap and the big dish form an interference fit and can be directly disassembled; the panel of the screw cap is provided with a first rubber stopper and a second rubber stopper; and a rubber packing ring is arranged in the screw cap. The culture chamber has the advantages of simple structure, convenient operation, wide applicability and capability of effectively collecting the radioactive gases.
Owner:SUZHOU UNIV

Method for identifying salt tolerance of barley in seedling stage

The invention discloses a method for identifying salt tolerance of barley in a seedling stage. Barley materials to be identified are germinated, after the seeds germinate, seedlings are transferred, salt treatment is then carried out, the chlorophyll content of the seedlings and the dry weight of overground parts are measured, and through data processing and in combination with product indicatorsof the relative chlorophyll content and the relative dry weight of the overground parts, a salt-resistant material can be rapidly and preliminarily identified. By the method, the barbey materials canbe repeatedly screened on a large scale, the occupied space is quite small, the repeatability and accuracy of experiments can be ensured effectively, the chlorophyll content and the dry weight of theoverground parts of the material are simply, rapidly and conveniently measured, and the method has important significance for large-scale screening of group materials.
Owner:SHANGHAI ACAD OF AGRI SCI

Method for cultivating attenuated strain pilio inactivated vaccine

The present invention is cultivation process of deactivated vaccine of attenuated polio strain. The cultivation process is completed with Vero cell and attenuated polio strain virus inside a fermentation tank. Through three stage amplification of cultivation scale, cell cultivating area equivalent to that of 21000 culturing glass bottle of 1 liter volume and cell output equivalent to that of 21000 culturing glass bottle of 1 liter volume may be obtained. The present invention can meet the requirement of large scale preparation of cell and virus liquid. The virus liquid of the present invention may be used to produce vaccine with stable quality, high safety and excellent immunogenicity.
Owner:INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI

Intelligent service application shooting training auxiliary system

The invention discloses an intelligent service application shooting training auxiliary system. The intelligent service application shooting training auxiliary system comprises an auxiliary target box which is reduced in an equal proportion mode according to shooting training conditions, hit collecting modules and an analysis and evaluation module; the auxiliary target box which is reduced in an equal proportion mode according to the shooting training conditions comprises a plurality of simulation targets used for simulating the movement of a shooting target in the service application process, the hit collecting modules are arranged in the effective areas of the simulation targets, the hit collecting modules collect the hit information of the simulation targets and send the hit information to the analysis and evaluation module, and the analysis and evaluation module carries out score evaluation through an analysis and evaluation model based on the shooter grade and the hit information; the auxiliary target box is used for replacing a real training scene, the training occupied space is saved, a plurality of groups of auxiliary target boxes can be additionally arranged, installation and arrangement are more convenient, and a plurality of groups of on-duty teams can carry out synchronous training; and the actual task characteristics of troops are combined, the whole process of real shooting actions is simulated and constructed, the data of trainees is collected and analyzed, and a basis and a follow are provided for formulating a training plan and improving the training level.
Owner:成都高阳信息技术有限公司

Intravenous infusion training system based on VR platform

The invention provides an intravenous infusion training system based on a VR platform. The system comprises a control host, a display device, a force feedback device and a foot-operated device. The control host is electrically connected with the display device, the force feedback device and the foot-operated device. The display device is used for displaying a venous blood sample acquisition model. The force feedback device is used for sending a first control signal to the control host according to operation of a user so as to carry out intravenous infusion simulation operation on a venous blood sample acquisition injection arm, and receiving feedback information of the control host to carry out force feedback on the user. The foot-operated device is used for sending a second control signal to the control host according to the operation of the user so as to carry out auxiliary operation on the intravenous infusion simulation operation. According to the invention, the intravenous infusion training system is achieved based on the virtual reality technology, so a problem of failure to carry out repeated trainings on invasive experiments in experiment teaching is solved; problems of poor simulation ability, incapability of resisting repeated puncturing, liquid leakage and mildewing of a current model are solved; and the provided system is an innovative and valuable supplement to current teaching modes.
Owner:GUANGDONG MEDICAL UNIV

Method for preparing newcastle disease inactivated vaccine, and product and application thereof

The invention discloses a method for preparing a newcastle disease inactivated vaccine, and a product and application thereof, belonging to the field of preparation and application of newcastle disease inactivated vaccines. The method for preparing the newcastle disease inactivated vaccine disclosed by the invention comprises the following steps of: (1), culturing animal cells by utilizing a cell factory; (2), inoculating newcastle disease virus, and proliferating the virus in the cell factory; and (3), harvesting the virus to prepare the inactivated vaccine. According to the invention, proliferation conditions of the newcastle disease virus in the cell factory including cell density, virus infection multiplicity, TPCK-pancreatin concentration and virus culture time are optimized; the newcastle disease virus cultured by the method disclosed by the invention has high titer, so that immune production requirements can be satisfied; the process is steady, so that the newcastle disease inactivated vaccine can be produced in a large scale; the newcastle disease inactivated vaccine prepared by the method disclosed by the invention is safe to chickens; 100% immunoprotection to attack of the newcastle disease virus can be realized; and thus, the newcastle disease inactivated vaccine can be used for preparing a medicine or a reagent for preventing or treating newcastle diseases.
Owner:JILIN ZHENGYE BIOLOGICAL PROD

Simulation operation device enabling multiple types of simulation operation platforms to share view displayer

The invention relates to a rail transit driving simulation device, in particular to a simulation operation device enabling multiple types of simulation operation platforms to share a view displayer. The simulation operation device comprises an annular screen used as the view displayer, a simulation operation work area, simulation operation storage areas, a conversion device and simulation operation platforms. The annular screen is arranged in front of the simulation operation work area; the simulation operation storage areas are evenly distributed around the conversion device, and each simulation operation storage area is connected with the conversion device through tracks I; the conversion device is connected with the simulation operation work area through tracks II, and the space between the tracks I is the same as the space between the tracks II; small wheels capable of moving along the tracks are arranged at the bottoms of the simulation operation platforms, the simulation operation platforms are placed on the simulation operation storage areas when the simulation operation platforms do not work, and the simulation operation platforms are moved to the simulation operation work area through the conversion device when the simulation operation platforms work. According to the simulation operation device enabling the multiple types of simulation operation platforms to share the view displayer, multiple types of simulation operation platforms can share the view displayer, the problems that existing devices leave unused, utilization rate is low and repeated purchase are solved, capital is saved, and practical training places are saved.
Owner:LIUZHOU RAILWAY VOCATIONAL TECHN COLLEGE

Incubator

The invention discloses an incubator. The incubator comprises a tray and a dividing fence which is arranged in the tray, used for dividing the tray into multiple grids and crossly formed by multiple mutually parallel cross baffles and multiple mutually parallel vertical baffles; and a gap for connecting the multiple grids exists between the lower end face of the dividing fence and the inner bottom face of the tray. The dividing fence in the incubator provided by the invention is used for dividing the tray into the multiple grids communicated with the bottom and solid culture mediums are distributed on the whole tray through the gap on the bottom of the dividing fence; each solid culture medium is higher than the bottom of the dividing fence, so that the solid culture grids with great number, same height and relatively independent space are formed, the bacterial culture flux is increased, the culture space is reduced, and the bacterium is easily marked.
Owner:BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION

Yard rose tissue culture seedling ex-vitro rooting method

The invention relates to a courtyard Chinese rose tissue culture seedling ex-vitro rooting method, which belongs to the technical field of Chinese rose propagation, and comprises the following steps: (1) matrix preparation and sterilization; (2) matrix filling preparation; (3) carrying out ex-vitro rooting culture; (4) sealing; and (5) culturing. According to the method, rooting culture of the courtyard roses is carried out in a mode of combining the ex-vitro rooting substrate and the 126-hole tray, so that the tissue culture procedure is simplified, the tissue culture space is saved, the use of plant hormones is reduced, and the rooting rate is relatively high.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

A kind of manufacturing method of anaerobic gas generating bag

The invention discloses a manufacturing method for an anaerobic atmosphere generation bag. The method comprises the following steps: (1) preparation of various ingredients: preparing the following ingredients in parts by weight: 1-100 parts of iron and magnesium ion, 1-100 parts of a carbonate, 10-100 parts of L-sodium ascorbate, 10-100 parts of powdered carbon, and 10-100 parts of agar; (2) mixing of various ingredients: stirring and uniformly grinding the various ingredients prepared in step (1), and sub-packaging; (3) packaging: finishing the preparation of a packaging bag provided with two-ply packs, wherein the inner pack is a ventilating bag and the outer pack is an air-proof bag; (4) manufacturing: uniformly stirring ingredients stirred and uniformly grinded in step (2), subpackaging in the inner packaging material according to required dosage by utilizing a packaging machine, and then conducting vacuum packaging rapidly by using the outer packaging material. According to the anaerobic atmosphere generation bag obtained through the adoption of the method, the operation is convenient, the reaction is rapid, the anaerobic atmosphere generation bag is convenient to use, the cultivation space is saved, the requirement for a cultivation vessel is low, and the manufacturing cost is greatly lowered.
Owner:青岛高科技工业园海博生物技术有限公司

Hand grenade throwing training auxiliary device

The invention belongs to the field of throwing training, and particularly relates to a grenade throwing training auxiliary device which comprises a base, a support is mounted at the top of the base, an electric control unit is mounted on the support, a display system is mounted at the top of the support, and a speed measuring system is mounted at the top of the display system. An angle sensor is rotatably mounted at the top of the base, a pull rope is connected to the angle sensor, and a training bomb is connected to the end, away from the angle sensor, of the pull rope. The hand grenade throwing drop point can be displayed on the display screen, a trainer can adjust the force and direction according to throwing data, the training efficiency can be effectively improved, meanwhile, the training field can be saved, hand grenade throwing training can be organized only in a very small field, and the training efficiency is improved. Meanwhile, accidental injuries caused by improper throwing of the coach grenade can be effectively reduced, and the device has wide application prospects in new soldier grenade throwing training.
Owner:中国人民解放军31620部队

Culture box for producing tissue engineering skin

The invention relates to a culture box for producing tissue engineering skin. The culture box comprises a bottom box with a liquid-changing groove, a box cover and culture brackets, wherein the culture brackets are spread in a single layer in the bottom box. When using the culture box of the invention, the box cover is only needed to be opened three times during the whole culture period so as to greatly reduce the probability that the product is polluted and largely increase the reliability during the culture process; during culture and production process, the culture space can be saved, the operation sequences can be reduced and the production efficiency can be increased so as to greatly reduce the production cost and realize mass production; the standardized production and operation are easy to realize and the product quality is very stable; and the culture box of the invention has lighter weight, is not easy to break up, and can realize automatic or semi-automatic manipulator operation during the culture and production of the tissue engineering skin, dramatically reduce the labor intensity, increase the production efficiency and provide the basis for the mass production of the tissue engineering skin.
Owner:张光晟 +1

A kind of powder-free cold water instant micro-dry medium and its preparation method

The invention discloses a powder-free cold water fast-dissolving type micro-dried culture medium and a preparation method. The powder-free dried culture medium comprises culture medium dry powder which is dispersed in a film layer capable of absorbing water to form gel. The preparation process of the culture medium does not require boiling dissolving and high-pressure steam sterilization, and theproduction is simple and convenient. When the culture medium is used, no dust falls. Hydrophilic film forming matter and siphoning between the formed dry powder culture medium structure and an upper covering and a bottom culture medium jointly make sample liquid dispersed and absorbed quickly, the sample liquid is not spilled easily, and no second pollution is caused.
Owner:GUANGDONG HUANKAI MICROBIAL SCI & TECH +1

Method for efficiently transplanting betula platyphylla tissue culture seedlings

The invention discloses a method for efficiently transplanting betula platyphylla tissue culture seedlings, and belongs to forest tree breeding. The method comprises the following steps: (1) inoculating tissue culture adventitious buds of betula platyphylla into a subculture strong seedling culture medium, and carrying out illumination culture to obtain clustered seedlings; (2) when the height of the clustered seedlings is 3-5cm, cutting off callus lumps at the lower parts of the clustered seedlings, then transferring the clustered seedlings into a rooting matrix of a tissue culture bottle, carrying out illumination culture, and after adventitious roots grow, carrying out step-by-step seedling hardening and transplanting; wherein 20-30 clustered seedlings are inoculated in each bottle of rooting substrate. The tissue culture process provided by the invention is high in operability, and a large number of betula platyphylla seedlings can be produced in a tissue culture room, which also plays a reference role in transplanting other forest tissue culture seedlings.
Owner:NORTHEAST FORESTRY UNIVERSITY

Simulated manipulation device for multi-model shared visual display

The invention relates to a rail transit driving simulation device, in particular to a simulation operation device enabling multiple types of simulation operation platforms to share a view displayer. The simulation operation device comprises an annular screen used as the view displayer, a simulation operation work area, simulation operation storage areas, a conversion device and simulation operation platforms. The annular screen is arranged in front of the simulation operation work area; the simulation operation storage areas are evenly distributed around the conversion device, and each simulation operation storage area is connected with the conversion device through tracks I; the conversion device is connected with the simulation operation work area through tracks II, and the space between the tracks I is the same as the space between the tracks II; small wheels capable of moving along the tracks are arranged at the bottoms of the simulation operation platforms, the simulation operation platforms are placed on the simulation operation storage areas when the simulation operation platforms do not work, and the simulation operation platforms are moved to the simulation operation work area through the conversion device when the simulation operation platforms work. According to the simulation operation device enabling the multiple types of simulation operation platforms to share the view displayer, multiple types of simulation operation platforms can share the view displayer, the problems that existing devices leave unused, utilization rate is low and repeated purchase are solved, capital is saved, and practical training places are saved.
Owner:LIUZHOU RAILWAY VOCATIONAL TECHN COLLEGE

An ECG signal classification method based on the combination of CNN and GRU based on self-encoding mode

An ECG signal classification method based on the combination of CNN and GRU in the self-encoding mode, by extracting the most representative features in the original signal, using CNN+GRU for feature extraction, saving space and saving a lot of training space, among which The GRU (Gated Recurrent Unit) used on the one hand solves the problem of gradient disappearance and gradient explosion due to RNN training. On the other hand, it has one less gate than LSTM, which is easier to calculate and can improve training efficiency. The advantage of GRU is that when When there are few training samples, it can be used to prevent overfitting. When there are many training samples, it can also save a lot of training time, and can improve the learning efficiency of the network and the accuracy of ECG signal recognition.
Owner:SHANDONG UNIV OF SCI & TECH +1

Incubator

The invention discloses an incubator. The incubator comprises a tray and a dividing fence which is arranged in the tray, used for dividing the tray into multiple grids and crossly formed by multiple mutually parallel cross baffles and multiple mutually parallel vertical baffles; and a gap for connecting the multiple grids exists between the lower end face of the dividing fence and the inner bottom face of the tray. The dividing fence in the incubator provided by the invention is used for dividing the tray into the multiple grids communicated with the bottom and solid culture mediums are distributed on the whole tray through the gap on the bottom of the dividing fence; each solid culture medium is higher than the bottom of the dividing fence, so that the solid culture grids with great number, same height and relatively independent space are formed, the bacterial culture flux is increased, the culture space is reduced, and the bacterium is easily marked.
Owner:BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION

Method for carrying out nutrient culture and seed collection on wild rice by using fresh cutting method

PendingCN113207451AGuaranteed fresh insertion timePromotes full maturityCalcareous fertilisersMagnesium fertilisersNutrientNutrition
The invention relates to a method for carrying out nutrient culture and seed collection on wild rice by using a fresh cutting method. The method includes the steps of selecting a milk-ripe stage ear of the current year of the wild rice, cutting the ear with 1-3 leaves as a fresh-cut ear, sequentially inserting the fresh-cut ear into a triangular flask filled with three different stages of nutrient solutions, namely a first stage, a second stage and a third stage, carrying out step-by-step culture, and laying plastic cloth below a culture bottle and is used for collecting fallen seeds. According to the method for carrying out nutrient culture and seed collection on the wild rice by using the fresh cutting method, the collected mature seeds reach 75% or above, the occupied space for culture is small, operation and management are easy and convenient, the difficult problem that the mature seeds of the wild rice are difficult to collect is solved, and a sufficient number of high-quality wild rice seed materials are provided for wild rice seed resource preservation, cross breeding and related scientific research work.
Owner:云南省农业科学院生物技术与种质资源研究所

A kind of intermittent submerged lily scale multiplication and rapid propagation method

The invention discloses an intermittent immersion type lily scale multiplication and rapid propagation method, which comprises the following steps: 1) peeling off the scales of lily seed bulbs, and performing surface sterilization; 2) inoculating the surface sterilized lily scales on a solid base The culture medium is cultured for 7-10 days to carry out the selection of sterile explants, and then the lily scales which have been determined to be pollution-free are placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture. The present invention has the advantages of high utilization rate of cultivation space and favorable exchange of cultivation gas components, thereby improving culture quality and reproduction coefficient, suitable for large-scale production; cultivation device and accessories are easy to be sterilized as a whole, the cultivation process is completely closed, and the probability of pollution is small; The structure is simple and compact, with low energy consumption, which saves the culture space and reduces the cost of tissue culture; it reduces the transfer and subculture operation of tissue culture seedlings in the overall tissue culture process, and saves labor and production costs.
Owner:INST OF BOTANY CHINESE ACAD OF SCI
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