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Method for carrying out nutrient culture and seed collection on wild rice by using fresh cutting method

A technology of medicinal wild rice and wild rice, applied in botany equipment and methods, vegetative propagation, rice cultivation, etc., can solve the problems of no public reports, easy to mix, scattered on the ground, etc., to improve the harvest rate and survival rate , easy to collect seeds, concentrated effect of ripening time

Pending Publication Date: 2021-08-06
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sometimes due to the limited cultivation space, multiple population materials are planted together, and the seeds fall after maturity and are scattered on the ground, which is easy to mix.
In particular, the medicinal wild rice plants are tall (usually more than 1.5 meters), lush, and the stems are extremely brittle and easy to break, which is extremely unfavorable for plant growth, preservation and seed collection, which has caused great harm to our related scientific research work. inconvenient
[0004] The present invention provides a method for vegetatively cultivating and collecting wild rice verruca and medicinal wild rice by using fresh cutting method. After literature search, there is no public report on the same technology as the present invention

Method used

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  • Method for carrying out nutrient culture and seed collection on wild rice by using fresh cutting method
  • Method for carrying out nutrient culture and seed collection on wild rice by using fresh cutting method
  • Method for carrying out nutrient culture and seed collection on wild rice by using fresh cutting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Experimental material: wild rice verruca in Jinghong, Yunnan.

[0027] In Example 1, one treatment and two comparisons were set. They are treatment 1, control 1, and control 2, respectively.

[0028] Treatment 1: vegetative culture of fresh-cut ears of wild rice verruca (the method of the present invention); control 1: non-vegetative culture of fresh-cut ears of wild rice verruca; control 2: pot culture of wild rice verruca.

[0029] Treating 1 fresh-cut ear vegetative culture of wild rice verruca: adopt the method of the present invention to carry out fresh-cut vegetative culture of wild rice verruca in Yunnan Jinghong by fresh cutting method until mature seeds, and collect seeds. Specific steps are as follows:

[0030] (1) Prepare the following nutrient solution

[0031] Nutrient solution A: NH 4 NO 3 1800mg / L+KNO 3 2000mg / L+CaCl 2 2H2 O 500mg / L+MgSO 4 ·7H 2 O400mg / L+KI 300mg / L;

[0032] Nutrient solution B: ZnSO 4 ·7H 2 O 4.3mg / L+H 3 BO 3 1mg / L+KI 0....

Embodiment 2

[0057] Experimental material: medicinal wild rice in Jinghong, Yunnan.

[0058] In Example 2, one treatment and two controls were set up, which were respectively recorded as treatment 2, control 3 and control 4.

[0059] Treatment 2: vegetative culture of fresh-cut panicles of medicinal wild rice (the method of the present invention); control 3: non-vegetative cultivation of fresh-cut ears of medicinal wild rice; control 4: pot culture of medicinal wild rice.

[0060] Process 2 medicinal wild rice fresh-cut ear vegetative culture (method of the present invention): take Yunnan Jinghong medicinal wild rice as experimental material, adopt the inventive method to carry out fresh-cut ear vegetative culture and seed collection, except step (2) culture material The selection is different from the vegetative culture of treating 1 fresh-cut ear of wild rice verruca in Example 1, and the rest of the steps are the same as the vegetative culture of treating 1 fresh-cut ear of wild rice ve...

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Abstract

The invention relates to a method for carrying out nutrient culture and seed collection on wild rice by using a fresh cutting method. The method includes the steps of selecting a milk-ripe stage ear of the current year of the wild rice, cutting the ear with 1-3 leaves as a fresh-cut ear, sequentially inserting the fresh-cut ear into a triangular flask filled with three different stages of nutrient solutions, namely a first stage, a second stage and a third stage, carrying out step-by-step culture, and laying plastic cloth below a culture bottle and is used for collecting fallen seeds. According to the method for carrying out nutrient culture and seed collection on the wild rice by using the fresh cutting method, the collected mature seeds reach 75% or above, the occupied space for culture is small, operation and management are easy and convenient, the difficult problem that the mature seeds of the wild rice are difficult to collect is solved, and a sufficient number of high-quality wild rice seed materials are provided for wild rice seed resource preservation, cross breeding and related scientific research work.

Description

technical field [0001] The invention relates to the technical field of plant hydroponics, in particular to a method for vegetatively cultivating wild rice and collecting seeds through hydroponics. Background technique [0002] Wild rice (Oryza rufipogon Griff.) is the original ancestor of cultivated rice. It has grown in the wild for a long time and has withstood the selection of various adversity stresses. It has rich genetic diversity and preserves a large number of excellent genes that cultivated rice does not have or has disappeared. academic and economic value. There are common wild rice (Oryza rufipogon Griff.), warty wild rice (Oryza meyeriana Nees et Arn.ex Hook.e.) and medicinal wild rice (Oryza officinalisWall.ex wstt) in China. Among them, wild rice verruca and medicinal wild rice are the resources with the most research value. They have very strong resistance to diseases and insect pests and stress resistance, and their genomes, genotypes, and cultivated rice an...

Claims

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Application Information

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IPC IPC(8): A01G2/00A01G22/22C05G3/00C05G5/20C05G1/00
CPCA01G2/00A01G22/22C05G3/00C05G5/20C05C1/00C05D1/00C05C11/00C05C5/02C05D3/00C05D5/00C05D9/02Y02P60/21
Inventor 钟巧芳王玲仙程在全陈玲王波肖素勤张墩宇殷富有柯学张云蒋聪雷涌涛余腾琼李娥贤邢佳鑫
Owner 云南省农业科学院生物技术与种质资源研究所
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