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A kind of manufacturing method of anaerobic gas generating bag

A production method and air bag technology, which are applied in the field of microorganism culture, can solve the problems of high container requirements, complicated operation, high temperature resistant containers, etc., and achieve the effects of saving culture space, rapid response, and reducing production costs.

Active Publication Date: 2016-03-30
青岛高科技工业园海博生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Anaerobic culture is often used in the process of microbial detection. At present, the more common methods in China are the candle jar method, the pyrogalla method, the palladium catalyzed method, and the use of anaerobic tanks to pump air. These methods have high requirements for containers and require patience. High temperature container, or need to add water and catalyst, the operation is more complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for making an anaerobic gas production bag, comprising the steps of:

[0022] (1) Preparation of various ingredients:

[0023] The following ingredients are prepared in parts by weight:

[0024] 1 part iron and magnesium ions;

[0025] 1 part carbonate;

[0026] 10 parts of sodium L-ascorbate;

[0027] 10 parts charcoal powder;

[0028] 10 parts of agar;

[0029] (2) Mixing various ingredients:

[0030] Stir and grind the various ingredients prepared in step (1) evenly, and pack them separately;

[0031] (3) Packaging:

[0032] Prepare the packaging bag with two layers of packaging, the inner layer is an air-permeable bag, and the outer layer is an air-tight bag;

[0033] (4) Production:

[0034] Stir the ingredients that have been stirred and ground evenly in step (2) evenly, use a filling machine to divide into the inner packaging material according to the required amount, and then quickly use the outer packaging material for vacuum packaging.

Embodiment 2

[0036] A method for making an anaerobic gas production bag, comprising the steps of:

[0037] (1) Preparation of various ingredients:

[0038] The following ingredients are prepared in parts by weight:

[0039] Iron and magnesium ions 50 parts;

[0040] Carbonate 50 parts;

[0041] 50 parts of sodium L-ascorbate;

[0042] 50 parts of charcoal powder;

[0043] 50 parts of agar;

[0044] (2) Mixing various ingredients:

[0045] Stir and grind the various ingredients prepared in step (1) evenly, and pack them separately;

[0046] (3) Packaging:

[0047] Prepare the packaging bag with two layers of packaging, the inner layer is an air-permeable bag, and the outer layer is an air-tight bag;

[0048] (4) Production:

[0049] Stir the ingredients that have been stirred and ground evenly in step (2) evenly, use a filling machine to divide into the inner packaging material according to the required amount, and then quickly use the outer packaging material for vacuum packaging....

Embodiment 3

[0051] A method for making an anaerobic gas production bag, comprising the steps of:

[0052] (1) Preparation of various ingredients:

[0053] The following ingredients are prepared in parts by weight:

[0054] 100 parts of iron and magnesium ions;

[0055] Carbonate 100 parts;

[0056] 100 parts of sodium L-ascorbate;

[0057] 100 parts of charcoal powder;

[0058] 100 parts of agar;

[0059] (2) Mixing various ingredients:

[0060] Stir and grind the various ingredients prepared in step (1) evenly, and pack them separately;

[0061] (3) Packaging:

[0062] Prepare the packaging bag with two layers of packaging, the inner layer is an air-permeable bag, and the outer layer is an air-tight bag;

[0063] (4) Production:

[0064] Stir the ingredients that have been stirred and ground evenly in step (2) evenly, use a filling machine to divide into the inner packaging material according to the required amount, and then quickly use the outer packaging material for vacuum pa...

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Abstract

The invention discloses a manufacturing method for an anaerobic atmosphere generation bag. The method comprises the following steps: (1) preparation of various ingredients: preparing the following ingredients in parts by weight: 1-100 parts of iron and magnesium ion, 1-100 parts of a carbonate, 10-100 parts of L-sodium ascorbate, 10-100 parts of powdered carbon, and 10-100 parts of agar; (2) mixing of various ingredients: stirring and uniformly grinding the various ingredients prepared in step (1), and sub-packaging; (3) packaging: finishing the preparation of a packaging bag provided with two-ply packs, wherein the inner pack is a ventilating bag and the outer pack is an air-proof bag; (4) manufacturing: uniformly stirring ingredients stirred and uniformly grinded in step (2), subpackaging in the inner packaging material according to required dosage by utilizing a packaging machine, and then conducting vacuum packaging rapidly by using the outer packaging material. According to the anaerobic atmosphere generation bag obtained through the adoption of the method, the operation is convenient, the reaction is rapid, the anaerobic atmosphere generation bag is convenient to use, the cultivation space is saved, the requirement for a cultivation vessel is low, and the manufacturing cost is greatly lowered.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a manufacturing method of an anaerobic gas producing bag which is specifically applied to the anaerobic cultivation of microorganisms and provides an anaerobic environment required for growth of microorganisms. Background technique [0002] Anaerobic culture is often used in the process of microbial detection. At present, the more common methods in China are the candle jar method, the pyrogalla method, the palladium catalyzed method, and the use of anaerobic tanks to pump air. These methods have high requirements for containers and require patience. High-temperature containers, or need to add water and catalyst, the operation is more complicated. Contents of the invention [0003] Aiming at the deficiencies in the prior art, the technical problem to be solved by the present invention is to provide a method for making an anaerobic gas-generating bag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
Inventor 蔡向荣
Owner 青岛高科技工业园海博生物技术有限公司
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