Cell culture chamber for collecting radioactive gases

Abstract

The invention discloses a cell culture chamber for collecting radioactive gases, which comprises a culture dish base, a screw cap and a non-screw cap, wherein the culture dish base comprises a small dish and a big dish; the edge of the big dish is provided with a thread structure; the bottom surfaces of the small dish and the big dish are concentric and fixed together; the screw cap can be screwed on the big dish; the non-screw cap and the big dish form an interference fit and can be directly disassembled; the panel of the screw cap is provided with a first rubber stopper and a second rubber stopper; and a rubber packing ring is arranged in the screw cap. The culture chamber has the advantages of simple structure, convenient operation, wide applicability and capability of effectively collecting the radioactive gases.

Description

Technical field [0001] The invention relates to a cell culture device, in particular to a cell culture device for collecting radioactive gas. Background technique [0002] Such as figure 1 As shown, the purpose of the existing device is to realize the collection of radioactive carbon dioxide produced by cell metabolism. [0003] The bottom liquid is the cell culture medium (Incubation Mixture), and the cells adhere to the bottom of the bottle and grow. The column in the middle of the bottom of the bottle is a support, which is used to support the cylindrical tube above. The tube is filled with carbon dioxide to collect liquid Hydroxoxide of Hyamine, and H is added to the side arm of the bottle. 2 SO 4 . The entire system is sealed with a rubber cover. [0004] Instructions: [0005] 1. Add 0.3ml 6N H to the side wall of the culture flask in advance 2 SO 4 , Seal the entire system with a rubber cover; [0006] 2. Use a medical syringe through the rubber cap to add 5ml of cells and cel...

AI Technical Summary

Problems solved by technology

[0011] 1. It is not easy to cultivate adherent cells. This device has no advantages for cultivating adherent cells: (1) It is not easy to add cell suspension; (2) It is not easy to change the medium; (3) It is not easy to digest cells and passage

[0012] 2. The volume is too large, it is not easy to put it into the incubator for batch cultivation, and it is difficult to meet the requirements of batch experiments

[0013] 3. The structure of the device is relatively complicated, and it is not easy to produce in batches, thus increasing the cost of a single device

[0014] 4. The whole device has a large space and requires a long shock time (more than 3 hours) to fully absorb radioactive carbon dioxide, and the whole requires additional shock equipment to achieve the purpose of full absorption

[0015] 5. The structure of the device is relatively complicated, and it is difficult to meet the sterility requirements required for cell culture, and the parts of the device are easily damaged during sterilization and handling

[0016] 6. The volume of the device is relatively large, and it is used as a radioactive experiment for one-time use, which increases the volume of radioactive waste accordingly

Images