Method for identifying salt tolerance of barley in seedling stage

Abstract

The invention discloses a method for identifying salt tolerance of barley in a seedling stage. Barley materials to be identified are germinated, after the seeds germinate, seedlings are transferred, salt treatment is then carried out, the chlorophyll content of the seedlings and the dry weight of overground parts are measured, and through data processing and in combination with product indicatorsof the relative chlorophyll content and the relative dry weight of the overground parts, a salt-resistant material can be rapidly and preliminarily identified. By the method, the barbey materials canbe repeatedly screened on a large scale, the occupied space is quite small, the repeatability and accuracy of experiments can be ensured effectively, the chlorophyll content and the dry weight of theoverground parts of the material are simply, rapidly and conveniently measured, and the method has important significance for large-scale screening of group materials.

Description

technical field [0001] The invention belongs to the field of identification of crop germplasm resources, and in particular relates to an identification method for the salt tolerance of barley at the seedling stage. Background technique [0002] Soil salinization is one of the most serious abiotic stress factors restricting global crop production and threatening food security. The transformation, development and utilization of saline-alkali land has become an important problem to be solved in the sustainable development of agriculture. [0003] Breeding new varieties of salt-tolerant crops through screening and genetic improvement is the most fundamental way to efficiently utilize saline-alkali land and improve comprehensive land productivity. Barley is the fourth largest cereal crop in the world and is widely used in feed, beer, food, and medicine. Because of its short growth period, wide adaptability and strong stress tolerance, it has been widely concerned by scientists a...

AI Technical Summary

Problems solved by technology

Over the years, researchers have conducted a large number of screening tests to explore the evaluation methods of plant salt tolerance; for example, in the bud stage, the germination rate, the length of the main root and the length of the coleoptile are mainly used as indicators to reflect the difference in salt tolerance between materials; During the whole growth period, the salt tolerance of materials is mainly screened through soil culture or hydroponic culture, and the differences of materials are reflected by indicators such as plant biomass and ion accumulation; in the whole growth period, the resistance of materials is mainly evaluated through field experiments with yield as an index. Salt tolerance, although these screening methods can identify differences in salt tolerance between certain species or a certain type of species, they all have certain limitations

[0005] Different varieties have different genetic backgrounds. In the method of germination identification, there are significant differences in the germination rate itself, and it is impossible to determine whether it is the problem of salt stress or the low germination rate itself; , using artificial simulation of salinity environment, by measuring the seedling height, root length and whole plant fresh weight of different treatments, to investigate the degree of salt damage of the samples to be tested under salt stress conditions relative to the blank control conditions, but the conditions need to emphasize the plant For the growth space and environment, if it is necessary to ensure that enough samples are repeated for statistical analysis, it will take up a lot of space; due to the influence of many external factors, it is difficult to control the salt concentration in the field test, which leads to poor repeatability of the test. The test cycle is long, time-consuming and labor-intensive, especially when large-scale screening of salt-tolerant varieties is difficult; other physiological indicators measure more sodium and potassium ion content, and reports have shown that barley cannot correctly reflect the differences among materials. Therefore, there is an urgent need for an effective and simple screening method

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