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Detoxification and cell pan rapid propagation method for purple sweet potatoes

A purple sweet potato and hole tray technology, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of increased dead seedling rate, small purple sweet potato pieces, and poor quality, so that it is not limited by seasons, Effects of prevention of species degradation and easy operation

Inactive Publication Date: 2015-07-22
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the expansion of the planting area of ​​purple sweet potatoes, the damage of purple sweet potatoes by the virus has become more and more serious. After the purple sweet potatoes are infected with the virus, the variety will degenerate, the rate of dead seedlings will increase, and the yield will decrease or even fail; the purple sweet potatoes infected with the virus will become smaller, poor quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The nationally approved purple sweet potato variety "Ningzishu No. 1" was selected for virus-free and rapid propagation. The specific steps are as follows:

[0023] 1) Stem tip disinfection: In mid-April, select healthy potato seedlings with typical characteristics of this variety, robust growth, and no pests and diseases on the sweet potato seedbed of "Ningzishu No. Put all the visible leaves into a clean triangular flask, rinse them repeatedly under tap water for 5 minutes, add two drops of dishwashing liquid and soak for 10 minutes, pour off the washing liquid, and then wash with distilled water for 5 times to remove the remaining washing liquid. Then the stem tip was transferred to the ultra-clean workbench, soaked in 75% ethanol for 30s for surface disinfection, washed twice with sterile water, and then the stem tip was soaked in 2% NaClO solution for 10 minutes and rinsed with sterile water 3 times, wash the residual NaClO solution, carry out deep disinfection, us...

Embodiment 2

[0033] The "Ningzishu No. 2" purple sweet potato variety was selected for detoxification and rapid propagation in plug trays. The specific steps are as follows:

[0034] 1) Stem tip disinfection: In mid-April, select healthy potato seedlings with typical characteristics of this variety, robust growth, and no pests and diseases on the purple sweet potato seedbed of "Ningzishu No. Remove all visible leaves, put it into a clean triangular flask, rinse it repeatedly under tap water for 10 minutes, add two drops of dishwashing liquid to soak for 10 minutes, pour off the washing liquid, and then wash it with distilled water 5 times to wash off the remaining washing liquid. Then transfer the stem tip to the ultra-clean workbench, soak it with 75% ethanol for 30s for surface disinfection, wash it twice with sterile water, then soak the stem tip in 2% NaClO solution for 10min, and then wash it with sterile water Rinse 3 times, wash off the residual NaClO solution, carry out deep disinfec...

Embodiment 3

[0044] The purple sweet potato variety "Zhe Zishu No. 1" was selected for detoxification and rapid propagation in plug trays. The specific steps are as follows:

[0045] 1) Disinfection of stem tips: Cut about 2 cm stem tips from purple sweet potato seedlings of "Zhe Zishu No. 1", remove all leaves and put them in a clean triangular flask, rinse them repeatedly under tap water for 8 minutes, add two drops of detergent Soak for 10 minutes, pour off the washing liquid, and wash 5 times with distilled water. Transfer the stem tip to an ultra-clean workbench, soak it with 75% ethanol for 30s, wash it with sterile water for 3 times, and then place the stem tip in 0.1% HgCl 2 Soak in the (mercuric chloride) solution for 5min, then rinse 3 times with sterile water, and the stem tip after disinfection is sucked off the moisture on the stem tip surface with sterile filter paper, and placed in a sterile petri dish for subsequent use;

[0046] 2) Stripping and inoculation of the shoot a...

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Abstract

The invention discloses a detoxification and cell pan rapid propagation method for purple sweet potatoes and belongs to the technical field of crop variety breeding. The method comprises the following steps: disinfecting stem tips of the purple sweet potatoes; peeling and inoculating meristems of the stem tips of the purple sweet potatoes; culturing the meristems of the stem tips of the purple sweet potatoes; selecting the cell pan and a substrate; transplanting and rapidly propagating a virus-free tube seedling of the purple sweet potatoes and the like. According to the method disclosed by the invention, the formula of a detoxification and regeneration culture medium of purple sweet potatoes is improved, and the method is convenient to operate and great in inoculum size. By using the cell pan, the rapid propagation speed of a virus-free seedling is greatly accelerated. Furthermore, the method is high in propagation coefficient and is not limited by seasons.

Description

technical field [0001] The invention relates to the field of plant tissue culture detoxification and rapid propagation, in particular to a method for purple sweet potato detoxification and plug rapid propagation. Background technique [0002] Purple sweet potato refers to a type of sweet potato whose flesh color is purple. It has rich nutritional value. In addition to the nutritional value of ordinary sweet potato, it is also rich in anthocyanins. Studies have shown that anthocyanins in purple potato tubers have strong antioxidant effects, can remove active oxygen, prevent high blood pressure and arteriosclerosis, improve liver function, reduce gene mutations, inhibit the production of carcinogens, and improve vision and other health care effects , and purple potato anthocyanins also have good heat and light resistance, and have broad application prospects in food, cosmetics, and medicine. Therefore, planting purple sweet potatoes has become more and more popular in recent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马佩勇边小峰贾赵东谢一芝郭小丁
Owner JIANGSU ACAD OF AGRI SCI
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