Method for rooting of horseradish outside tissue culture seedling bottle

A technique for tissue culture seedlings and exogenous rooting, which is applied in the field of plant reproduction, can solve the problems of long reproduction cycle and high production cost, and achieve the effects of lowering costs, lowering production costs and shortening the reproduction cycle.

Inactive Publication Date: 2012-09-19
JINGGANGSHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

But what existing wasabi tissue culture technology adopts is the rooting method in the bottle, and this method comprises five stages such as primary culture, subculture, rooting in the bottle, acclimatization and transplanting, has the problem that the reproduction cycle is long and the production cost is high

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  • Method for rooting of horseradish outside tissue culture seedling bottle

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Embodiment Construction

[0012] The growth of adventitious buds has a great influence on the rooting rate. The adventitious buds are taken from the top buds or side buds of wasabi, washed with tap water, peeled off the outermost layer under aseptic conditions, and then sterilized with 70% alcohol for 30-60 s, then took the shoot tip under a microscope, and inoculated In adventitious bud induction medium (MS + NAA 0.5 mg·L -1 + 6-BA 0.5 mg·L -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , pH 5.8), cultured at 17±1°C, inoculated in subculture medium ( MS + 6-BA 1 mg·L every 25 days -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , PH 5.8) to subculture once. When the sprouts grow to about 2cm, loosen the bottle cap of the culture bottle, train the seedlings in the acclimatization room for 3 days, then take out the rootless seedlings from the bottle, clean the remaining medium, and choose vigorous, strong and indeterminate sprouts with a length of about 2 cm. The sprouts are the material. Cut the sprouts into si...

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Abstract

The invention discloses a method for rooting of horseradish outside a tissue culture seedling bottle. The method is characterized by comprising the following steps of: utilizing tissue culture to induce the stem and the tip of the horseradish to obtain a rootless bud in a test tube; after subculture, domesticating the bud and cutting the bud to obtain a simple bud; inserting the bud into a soil matrix; and applying root promoter and 1/2 MS nutrient solution to the bud each week. After being inserted in the soil matrix for 30 days, the bud can have a good rooting effect. The technology greatly shortens the production cycle of tissue culture seedlings of the horseradish and reduces the production cost.

Description

technical field [0001] The invention relates to a plant propagation method, and mainly relates to a method for rooting out of a bottle of wasabi tissue culture seedlings. Background technique [0002] Wasabi ( Wasabia japonica Matsum.) is a plant of the genus Brassicaceae, and the slurry made from its rhizome is the raw material for seasoning food green mustard. Wasabi is not only fragrant and delicious, but also a special edible health plant, which has the effects of stimulating appetite, inhibiting bacteria, killing insects, and fighting cancer. Because wasabi has very strict requirements on environmental conditions, it can only grow in cold mountainous areas where the humid water quality is colder and clearer. The places suitable for its growth and planting are limited, resulting in the extreme scarcity of wasabi products in the international market. Seedlings are the key to the industrial production of wasabi. Adopting the method of tissue culture to propagate horse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 曾建军
Owner JINGGANGSHAN UNIVERSITY
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