A kind of powder-free cold water instant micro-dry medium and its preparation method

Abstract

The invention discloses a powder-free cold water fast-dissolving type micro-dried culture medium and a preparation method. The powder-free dried culture medium comprises culture medium dry powder which is dispersed in a film layer capable of absorbing water to form gel. The preparation process of the culture medium does not require boiling dissolving and high-pressure steam sterilization, and theproduction is simple and convenient. When the culture medium is used, no dust falls. Hydrophilic film forming matter and siphoning between the formed dry powder culture medium structure and an upper covering and a bottom culture medium jointly make sample liquid dispersed and absorbed quickly, the sample liquid is not spilled easily, and no second pollution is caused.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection and a preparation method thereof. Background technique [0002] In the 17th century, Anton van Leeuwenhoek and Robert Hooke successively discovered the existence of bacteria and filamentous fungi. Since then, the microscope and the culture method of microorganisms have become two urgent problems in the development of microbiology. In 1860, Pasteur used yeast waste residue, sugar and amino salt medium to cultivate bacteria in the laboratory for the first time. Agar was first discovered in 1658 by the Japanese Minora Tarazaemon. In 1882, Walter Hesse introduced agar to Koch, who became the first microbiologist to use agar. In the early 20th century, agar began to replace gelatin as a medium coagulant and has been used ever since. However, the agar medium needs to be heated and boiled above 85°C to dissolve, then sterilized by high-pressure steam at 121°C for 15 minutes, poured into...

AI Technical Summary

Problems solved by technology

However, this series of products are made of adhesive powder and coating, and dust will fall during use, causing secondary pollution to the inspected space environment, equipment and inoculation operation space.

In addition, due to the technical problems of the product itself, the absorption and diffusion of the sample liquid are slow after adding the sample liquid, and a pressure plate is required to spread the sample liquid and limit it within the specified range, and it is easy to overflow, resulting in inaccurate test results and pollution to the operating space.

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