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High-efficiency propagation method of hibiseu manihot plant

A technology of golden sunflower and plant, applied in the field of high-efficiency breeding of golden sunflower plants, can solve problems such as unreported golden flower sunflower, and achieve the effects of improving seedling breeding coefficient, high reproductive coefficient, and cumbersome seedling raising process.

Active Publication Date: 2018-04-24
河南芙罗拉农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research on the breeding of goldenflower sunflower using plant tissue culture technology.

Method used

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  • High-efficiency propagation method of hibiseu manihot plant
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  • High-efficiency propagation method of hibiseu manihot plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method for the high-efficiency breeding of the sunflower sunflower plant of the present embodiment may further comprise the steps:

[0028] (1) Take mature and plump sunflower seeds, wash them with running water for 10 minutes, sterilize them with 75% absolute ethanol for 60 seconds in a sterile operating table, then sterilize them with 25% sodium hypochlorite solution for 5 minutes, and then use a 600W ultrasonic instrument to sonicate them 4min, finally rinsed with sterile water 6 times, filter paper to dry the moisture on the seed surface, set aside.

[0029] (2) inoculate the sunflower sunflower seeds after the disinfection in the step (1) in the seed germination medium, be 24 ℃, light intensity 2500lx in temperature, the constant temperature cultivation room that photoperiod is 16 / 10h (light / dark) carries out Germination induction, after 2 weeks of light cultivation, obtain a complete seed seedling plant with a height of 2.0 to 5.0 cm and 2 to 3 true leaves, su...

Embodiment 2

[0034] This embodiment tested different concentrations of 6-BA and GA 3 To the influence of the golden flower sunflower seed germination effect in the above-mentioned embodiment implementation process, concrete operation is as follows:

[0035] The sterilized goldenflower seeds were inoculated with different concentrations of 6-BA (0, 0.05, 0.1, 0.2, 0.5mg / L) and GA 3 (0, 0.02, 0.1, 0.2 mg / L) MS medium, in a constant temperature culture room with a temperature of 24° C., a light intensity of 2500 lx, and a light time of 16 h for seed germination induction. After 2 weeks of cultivation, the germination rate and growth of the seeds were counted. The results are shown in Table 1.

[0036] Table 1 Different concentrations of 6-BA and GA 3Effects on germination of sunflower seeds

[0037]

[0038] The results in Table 1 show that when 6-BA is added alone in the medium, as the concentration of 6-BA increases, the germination rate of the seeds gradually increases first and the...

Embodiment 3

[0040] The present embodiment has tested the influence of different concentrations of IBA and spermidine on the rooting effect of the adventitious buds of Anemone annuus, and the specific operation is as follows:

[0041] The elongated adventitious buds were transferred to 1 / 4MS medium supplemented with different concentrations of IBA (0.1, 0.2, 0.5, 1.0 mg / L) and spermidine (5.0, 10.0, 15.0, 20.0 mg / L) After 3 weeks of light cultivation, the rooting rate, the average number of roots produced by each explant and the average root length were counted. The results are shown in Table 2.

[0042] Table 2 Effects of different concentrations of IBA and spermidine on rooting effect of adventitious buds

[0043]

[0044] The results in Table 2 show that when IBA is added alone in the medium, as the concentration of IBA increases, the germination rate of the seeds first gradually increases and then decreases. Excellent, the rooting rate is 87.5%. Further studies have found that th...

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Abstract

The invention discloses a high-efficiency propagation method of a hibiseu manihot plant. The method comprises the following steps: collecting mature and full seeds of hibiseu manihot as an initial material, and performing the surface disinfection; inoculating the disinfected seeds into a germination culture medium, performing the lighting culture, and obtaining aseptic seedlings; under aseptic condition, cutting stems of the aseptic seedlings of the hibiseu manihot, inoculating into an adventitious bud induction culture medium, inducing adventitious buds at incisions on two ends of the stem after lighting culture; transferring the stems with the adventitious buds into an elongation culture medium, and performing the elongation culture of the adventitious buds; and when a full leaf of the adventitious bud grows, transferring the adventitious buds into a rooting culture medium, performing the rooting culture, and after the lighting culture, acquiring test-tube seedlings of the hibiseu manihot with strong root system. By adopting the method, the propagation of the seedlings of the hibiseu manihot does not need the seed seedling growth, so that the time limit of the hibiseu maniht seedling is solved, the seedling propagation coefficient is increased, and a foundation is set for the large-scale propagation and variety improvement research of the later excellent plants of the hibiseumanihot.

Description

technical field [0001] The invention relates to a method for propagating goldenflower sunflower, in particular to a method for efficiently breeding goldenflower sunflower plants. Background technique [0002] Golden flower sunflower (scientific name: Hibiseu manihot L.), also known as: vegetable hibiscus, also known as: wild hibiscus, is an annual herbaceous Malvaceae okra plant. In August 2003, the Chinese Academy of Agricultural Sciences discovered an endangered plant in Xingtai, Hebei. Among the more than 200 plants of the genus Okra, goldenflower has not only ornamental value, but also edible, medicinal and health care functions. It is also adaptable. Extremely strong, cold-resistant, heat-resistant, and salt-alkali-resistant, it can be planted in areas with high temperatures of 40°C and low temperatures of -10°C, and has extremely high development potential and utilization value. [0003] Sunflower can be planted in gardens, industrialized in gardens and fields, and ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 侯金艳吴丽芳
Owner 河南芙罗拉农业科技有限公司
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