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Method for culturing strawberry virus-free seedlings through tissue culture technology

A technology of virus-free seedlings and strawberries, applied in horticultural methods, botany equipment and methods, applications, etc., can solve the problems of low survival rate of transplanting, low reproduction coefficient, pollution, etc., and achieve the convenience of transplanting and planting management, The tissue culture process is simple and the transplanting survival rate is high

Active Publication Date: 2016-09-21
四川源乡贝瑞农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not easy to cultivate strawberry virus-free female parent seedlings. The selection of explants for tissue culture is defective, and the condition control in the cultivation process is insufficient, etc., which will cause poor detoxification effect, pollution, vitrification, low reproduction coefficient, and poor transplant survival rate. Not high-level problems, causing great losses to production

Method used

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  • Method for culturing strawberry virus-free seedlings through tissue culture technology
  • Method for culturing strawberry virus-free seedlings through tissue culture technology
  • Method for culturing strawberry virus-free seedlings through tissue culture technology

Examples

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Effect test

Embodiment 1

[0029] A kind of method utilizing tissue culture technology to cultivate virus-free strawberry seedlings described in the present embodiment comprises the following steps:

[0030] (1) Selection and sterilization of explants: collect unopened flower buds of strawberry plants of Xuemei variety, the size of the flower buds is 0.4-0.6 mm, first sterilize with 75% alcohol for 20 seconds, and then use 0.1% mercuric chloride solution Sterilize for 13 minutes, take out the anthers, and obtain explants;

[0031] (2) Cluster bud induction: inoculate the explants obtained in step (1) into medium M1, culture in the dark for 9 days, and then cultivate in light for 80 days, until cluster shoots are produced, and the bud height of the cluster buds is not less than 2 cm. The culture conditions are culture light 2000-3000LX, culture temperature 24-26°C, and light duration 16h / day; the composition of the medium M1 is: MS basic medium supplemented with 1.0mg / L TDZ and 0.2mg / L IAA;

[0032] (3)...

Embodiment 2

[0035] A kind of method utilizing tissue culture technology to cultivate strawberry virus-free seedlings described in the present embodiment differs from the method described in Example 1 in that:

[0036] Step (2) clustering bud induction: Inoculate the explants obtained in step (1) into medium M1, culture in the dark for 9 days, then light culture for 80 days, until clustering buds are produced, and the height of the clustering buds is not less than 2cm, the culture conditions are culture light 2000-3000LX, culture temperature 24-26℃, light duration 16h / day; the composition of the medium M1 is: MS basic medium, supplemented with 1.5mg / L TDZ, 0.1mg / L IAA.

[0037]The method described in this example cultivates virus-free strawberry seedlings. When the explants are inoculated in medium M1 for culture, the callus generation time is 9 days of dark culture and then 10 days of light culture, and buds appear after 60 days of light culture. After 80 days of cultivation, the bud poi...

Embodiment 3

[0039] A kind of method utilizing tissue culture technology to cultivate strawberry virus-free seedlings described in the present embodiment differs from the method described in Example 1 in that:

[0040] Step (2) clustering bud induction: Inoculate the explants obtained in step (1) into medium M1, culture in the dark for 9 days, then light culture for 80 days, until clustering buds are produced, and the bud height of the clustering buds is not less than 2cm, the culture conditions are culture light 2000-3000LX, culture temperature 24-26℃, light duration 16h / day; the composition of the medium M1 is: MS basic medium, supplemented with 1.0mg / L TDZ, 0.2mg / L IAA.

[0041] The method described in this example cultivates virus-free strawberry seedlings. When the explants are inoculated in medium M1 for culture, the callus generation time is 9 days of dark culture and then 10 days of light culture, and buds appear after 60 days of light culture. After 80 days of cultivation, the bu...

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Abstract

The invention discloses a method for culturing strawberry virus-free seedlings through a tissue culture technology. The method includes the steps of firstly, selecting and sterilizing an explant, wherein unopened buds of a strawberry plant are collected, after the buds are sterilized and processed with alcohol and corrosive sublimate, anther is taken out, and the explant is obtained; secondly, inducing cluster buds, wherein the explant obtained in the first step is inoculated in a culture medium M1 to be cultured in the dark for 8-10 days and then cultured in light for 80-90 days till the cluster buds are produced, and the bud height of the cluster buds is not smaller than 2 cm; thirdly, transferring the cluster buds for culture, wherein the cluster buds obtained in the second step are transferred into a culture medium M2 to be cultured for 15-25 days till the cluster buds grow to strawberry seedlings which are 3-5 cm high, and then the strawberry seedlings are transferred into a culture medium M3 to be subjected to rooting culture to obtain the strawberry virus-free seedlings. The applied culture media are simple in formula, the tissue culture process is simple, the virus-free rate reaches 100%, the reproduction coefficient is high, the transplanting survival rate is high, seedlings are neat, later unified transplanting and planting management is convenient, and industrial production can be achieved.

Description

technical field [0001] The invention relates to the technical field of biological group cultivation seedlings, in particular to a method for cultivating virus-free strawberry seedlings using tissue culture technology. Background technique [0002] Strawberry is a perennial evergreen herbaceous plant belonging to the Rosaceae Strawberry genus. It belongs to polyploidy and is a crop that reproduces asexually by stolons. It is beneficial to the promotion of fine varieties, and is extremely susceptible to viruses. Strawberry mottle virus (SMOV), strawberry mild yellow edge virus (SMYEV), strawberry shrinkage virus (SCRV) and strawberry vein virus (SVBV) have been identified in my country. The widespread virus infection of seedlings is a bottleneck problem in strawberry production. Strawberries infected with viruses are weaker than non-infected strawberries, with poorer resistance, significantly lower yield, and worse fruit quality and commodity traits. For viral diseases, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 丁龙梅罗琳蔡世林栗丹李飞
Owner 四川源乡贝瑞农业发展有限公司
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