The invention discloses a
tissue culture and rapid propagation method of
Hibiscus mutabilis Linn. The
tissue culture and rapid propagation method comprises: (1)
bud induction culture:
cutting Hibiscusmutabilis Linn stem section with
bud point as explant, sterilizing, inoculating into an induction culture medium, and culturing until axillary buds germinate and grow to achieve a certain length; (2)rooting culture:
cutting the axillary buds obtained in the step (1), inoculating into a rooting culture medium, and culturing to form rooted
seedling; (3) proliferation culture:
cutting the single-
bud stem section or stem apex of the rooted
seedling obtained in the step (2), inoculating into a proliferation culture medium, and culturing to form a
Hibiscus mutabilis Linn
seedling; and (4) seedlingexercising and
transplantation: transferring the
Hibiscus mutabilis Linn seedling obtained in the step (3) to the outside, carrying out closed flask seedling exercising for 2-3 d,
transplanting to aseedbed, and carrying out film covering and sunshade net covering culture for 8-12 d until the new root of the
Hibiscus mutabilis Linn seedling grows, and removing the film covering and sunshade net covering culture. According to the present invention, with the
tissue culture and rapid propagation method, the growth can be promoted, the
survival rate of the cultured seedlings is high, the rootingrate is up to 100%, the culture time is short, and the rapid propagation of
Hibiscus mutabilis Linn can be achieved.