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A kind of tissue culture rapid propagation method of Hibiscus hibiscus

A technology for tissue culture and rapid propagation and Hibiscus can be applied to horticultural methods, botanical equipment and methods, plant regeneration and other directions, which can solve the problems of long breeding time, lack of promotion, low survival rate and the like, and achieve short and shortened cultivation time. The effect of reproductive cycle and high survival rate

Active Publication Date: 2020-05-08
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing hibiscus breeding and cultivation techniques are generally cutting or grafting methods, the breeding time is long, the survival rate is low, and cannot be popularized

Method used

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  • A kind of tissue culture rapid propagation method of Hibiscus hibiscus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The tissue culture rapid propagation method of a kind of hibiscus hibiscus described in the present embodiment, comprises the following steps:

[0034] (1) Bud induction culture: cut the stem section with bud point of Hibiscus hibiscus as explant, after cleaning, first treat it with 75% alcohol for 30 seconds, wash it twice with sterile water, and then treat it with 0.1% mercuric chloride for 9 minutes, Wash 4 times with sterile water, and finally absorb the water with sterile filter paper, inoculate it into the induction medium after adjusting the pH to 6.0 and sterilize, and cultivate until the upper axillary buds of the explants germinate and grow to a certain length, wherein The composition of the induction medium is: MS basic medium, supplemented with 0.2mg / L 6-BA, 0.1mg / L NAA, 20g / L sucrose, 6g / L agar;

[0035] (2) Rooting culture: cut out the axillary buds described in step (1), inoculate them into the rooting medium and cultivate them until the base of the axill...

Embodiment 2

[0041] The tissue culture rapid propagation method of a kind of hibiscus hibiscus described in the present embodiment, the difference with embodiment 1 is:

[0042] The composition of step (1) induction medium is: MS minimal medium, supplemented with 0.1mg / L 6-BA, 0.05mg / LNAA, 10g / L sucrose, 4g / L agar;

[0043] The composition of step (2) rooting medium is: 1 / 2MS minimal medium, supplemented with 0.1mg / L NAA, 0.05mg / L6-BA, 10g / L sucrose, 4g / L agar;

[0044] The composition of the proliferation medium in step (3) is: 1 / 2 MS basic medium supplemented with 0.1 mg / L NAA, 0.05 mg / L 6-BA, 10 g / L sucrose, and 4 g / L agar.

[0045] In the present embodiment, after step (1) bud induction culture for 2 weeks, the axillary buds germinated on the explants grow to 1-2 cm; after step (2) rooting culture for 10 days, the base of the axillary buds take root, and the root length is 2-2 cm after 3 weeks. 3cm, the rooting rate is 100%, and the upper axillary buds can be extended to 4cm, and afte...

Embodiment 3

[0047] The tissue culture rapid propagation method of a kind of hibiscus hibiscus described in the present embodiment, the difference with embodiment 1 is:

[0048] The composition of step (1) induction medium is: MS minimal medium, supplemented with 0.5mg / L 6-BA, 0.15mg / LNAA, 30g / L sucrose, 6g / L agar;

[0049] The composition of step (2) rooting medium is: 1 / 2MS basic medium, supplemented with 0.5mg / L NAA, 0.15mg / L6-BA, 30g / L sucrose, 6g / L agar;

[0050] The composition of the proliferation medium in step (3) is: 1 / 2 MS basic medium supplemented with 0.5 mg / L NAA, 0.15 mg / L 6-BA, 30 g / L sucrose, and 6 g / L agar.

[0051] In this example, after step (1) bud induction culture for 2 weeks, the axillary buds germinated on the explants grew to 2 cm; step (2) after rooting culture for 1 week, the base of the axillary buds took root, and the root length was 2-4 cm after 3 weeks , the rooting rate is 100%, and the upper axillary buds can be extended to 4cm, and the axillary buds can ...

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Abstract

The invention discloses a tissue culture and rapid propagation method of Hibiscus mutabilis Linn. The tissue culture and rapid propagation method comprises: (1) bud induction culture: cutting Hibiscusmutabilis Linn stem section with bud point as explant, sterilizing, inoculating into an induction culture medium, and culturing until axillary buds germinate and grow to achieve a certain length; (2)rooting culture: cutting the axillary buds obtained in the step (1), inoculating into a rooting culture medium, and culturing to form rooted seedling; (3) proliferation culture: cutting the single-bud stem section or stem apex of the rooted seedling obtained in the step (2), inoculating into a proliferation culture medium, and culturing to form a Hibiscus mutabilis Linn seedling; and (4) seedlingexercising and transplantation: transferring the Hibiscus mutabilis Linn seedling obtained in the step (3) to the outside, carrying out closed flask seedling exercising for 2-3 d, transplanting to aseedbed, and carrying out film covering and sunshade net covering culture for 8-12 d until the new root of the Hibiscus mutabilis Linn seedling grows, and removing the film covering and sunshade net covering culture. According to the present invention, with the tissue culture and rapid propagation method, the growth can be promoted, the survival rate of the cultured seedlings is high, the rootingrate is up to 100%, the culture time is short, and the rapid propagation of Hibiscus mutabilis Linn can be achieved.

Description

technical field [0001] The invention relates to the technical field of tissue culture and rapid propagation of plants, in particular to a method for tissue culture and rapid propagation of Hibiscus hibiscus. Background technique [0002] Hibiscus is native to the Yellow River Basin to South China, and is distributed in Hunan, Sichuan, Yunnan, Guangdong and other provinces. It has a cultivation history of nearly 3,000 years in our country. At present, it is mainly planted in Hunan, Sichuan and other places. Hibiscus is also the city flower of Chengdu. People often plant hibiscus near pools. In addition to being used as an ornamental, the flowers and leaves of hibiscus hibiscus can be used as medicine, which has the effects of clearing away heat, detoxifying, and reducing swelling. Existing Hibiscus hibiscus propagation and cultivation techniques are generally cuttage or grafting methods, the propagation time is long, the survival rate is low, and cannot be popularized. Con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 栗丹曾俊刘少奎
Owner SICHUAN COLORLINK CO LTD
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