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Tissue culture method for Cymbidium floribundum

A technology of tissue culture and cinnabar orchid, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of no cinnabar orchid tissue culture, etc., and achieve the advantages of easy transplanting and planting management, neat seedlings, and high multiplication frequency Effect

Active Publication Date: 2012-07-18
云南山里红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the tissue culture of cinnabar orchid in the current literature

Method used

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  • Tissue culture method for Cymbidium floribundum
  • Tissue culture method for Cymbidium floribundum
  • Tissue culture method for Cymbidium floribundum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Explant collection

[0056] October-November is the period when the capsules of cinnabar orchids are mature, and they are harvested when the capsules turn brown in appearance and reach maturity but have not yet cracked. If the time is too early, the germination rate will be affected by the underdevelopment of the seed embryo; if the time is too late, the capsule will rupture, which will cause unnecessary losses;

[0057] (2) Disinfection and sterilization

[0058] Take the uncracked capsules of cinnabar orchid, wash them with running water for 25 minutes, treat them with 75% alcohol for 25 seconds on the ultra-clean workbench, then soak them in 0.1% mercuric chloride solution for 10 minutes, pour off the mercuric chloride solution, and rinse them with sterile water 4 times. The second time should not be less than 4 minutes, and then dry the surface moisture of the fruit with sterile paper;

[0059] (3) Sowing

[0060] MS medium is the best for germination and gro...

Embodiment 2

[0068] (1) Explant collection

[0069] October-November is the period when the capsules of cinnabar orchids are mature, and they are harvested when the capsules turn brown in appearance and reach maturity but have not yet cracked. If the time is too early, the germination rate will be affected by the underdevelopment of the seed embryo; if the time is too late, the capsule will rupture, which will cause unnecessary losses;

[0070] (2) Disinfection and sterilization

[0071] Take the uncracked capsules of cinnabar orchid, wash them with running water for 30 minutes, treat them with 75% alcohol for 30 seconds on the ultra-clean workbench, then soak them in 0.1% mercuric chloride solution for 15 minutes, drain the mercuric chloride solution, and rinse them with sterile water for 5 times. The second time is not less than 5min, and finally the water on the fruit surface is blotted dry with sterile paper.

[0072] (3) Sowing

[0073] MS medium is the best for germination and gro...

Embodiment 3

[0081] (1) Explant collection

[0082] October-November is the period when the capsules of cinnabar orchids are mature, and they are harvested when the capsules turn brown in appearance and reach maturity but have not yet cracked. If the time is too early, the germination rate will be affected by the underdevelopment of the seed embryo; if the time is too late, the capsule will rupture, which will cause unnecessary losses;

[0083] (2) Disinfection and sterilization

[0084] Take uncracked capsules of cinnabar orchid, wash them with running water for 35 minutes, treat them with 75% alcohol for 35 seconds on an ultra-clean workbench, then soak them in 0.2% mercuric chloride solution for 15 minutes, drain the mercuric chloride solution, and rinse them with sterile water for 5 times. The second time is not less than 5min, and finally the water on the fruit surface is blotted dry with sterile paper.

[0085] (3) Sowing

[0086] MS medium is the best for germination and growth o...

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Abstract

The invention relates to a tissue culture method for a plant, in particular to a tissue culture method for Cymbidium floribundum, and belongs to the technical field of agricultural plantation. The tissue culture method for the Cymbidium floribundum comprises the following six steps of: collecting an explant, disinfecting and sterilizing, sowing, proliferating, strengthening seedlings, and performing rooting culture. When the Cymbidium floribundum is propagated through plant tissue culture by a tissue culture technology, the advantage of rapid propagation can be exerted, and a large number of seedlings are obtained in a short time; compared with cottage, the tissue culture method provided by the invention is characterized in that flower colors of plants are seldomly mutated in later culture; and the plant tissue culture serving as a vegetative propagation mode can ensure that the seedlings are regular, facilitates late unified transplantation and plantation management, and can also ensure that various characters of grown product plants and bonsai are consistent. Compared with the conventional propagation mode, the invention has the advantages that a used culture medium is short in induction time, quick in seedling emergence and high in proliferation frequency; and particularly, the seedlings are regular, the method is easy to operate, and the Cymbidium floribundum can be produced on a large scale.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a cinnabar orchid tissue culture method. It belongs to the technical field of agricultural planting. Background technique [0002] Cinnabar orchid is a plant of the genus Orchidaceae, named for its red flower color resembling cinnabar; in the Ming Dynasty, cinnabar orchid was used as a tribute, so it was also called "tribute orchid" and "shenpin orchid". The cinnabar orchid plant is vigorous, with thick and long roots. It blooms from September to October every year (usually around the Double Ninth Festival, and a few extend to the end of the year, or even around the Spring Festival). The flowering period is about January; the scape is 30-40cm high, and the flower stem is rust red. , with 3-5 flowers per scape, flower diameter 6-8 cm, willow leaf petals, sepals lanceolate, arranged in an equilateral triangle, cinnabar red to purple, pure fragrance, white tongue with small bright r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 岳健姚云芳李昆华李建李江黎
Owner 云南山里红生物科技有限公司
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