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Multilayer compound eccentric-wear-preventing continuous sucker rod and manufacturing device and method thereof
ActiveCN104060944AAccelerated corrosionImprove partial wear resistanceDrilling rodsLayered productsEpoxyManufacturing technology
The invention relates to a multilayer compound eccentric-wear-preventing continuous sucker rod and a manufacturing device and a manufacturing method thereof. The sucker rod is characterized in that one section or several sections of the external surface of a fiber reinforced resin matrix composite rod body are respectively covered with a thermosetting wear layer; each thermosetting wear layer is made from one of polyurethane, a fluorocarbon coating, modified epoxide resin, vinylester resin, unsaturated polyester and phenolic resin; by adopting a manufacturing technology of multilayer composite winding and pultrusion or weaving and pultrusion, an internal layer of longitudinal fibers, an intertwining layer or a weaving layer and an external layer of longitudinal fibers are formed from interior to exterior in sequence; the manufactured rod body has a continuous length of 0-5000m. The sucker rod has the beneficial effects that the radial strength and the axial compressive strength are enhanced; the corrosion resistance and the eccentric wear resistance of the thermosetting wear layers are improved by 30%; the maximum temperature tolerance of the thermosetting wear layers is up to 190 DEG C; meanwhile, the bonding between an sucker rod body and each thermosetting wear layer is greater in length and quite high in strength, so that the slippage and the layering of an eccentric-wear-preventing layer from the surface of the sucker rod in the normal operation of an oil well are avoided.
Owner:SHENGLI OIL FIELD XINDA PIPE IND TECH DEV CO LTD
Clonal tea tree breeding method
The invention mainly aims to provide a clonal tea tree breeding method comprising the following steps: a, selecting a garden; b, trenching and grooving; c, cutting and seedling growing; d, nursery garden management; tea seedling using the seedling growing method is high in cutting survival rate, and breeding inherent characteristics can be well kept, so progeny properties are consistent, breeding period and growth vigor are tidy, and new shoot size, shoot tenderness0keeping ability and colors are close; subsequent mechanical tea harvesting is convenient, and primary processing technology can be standardized; the breeding method is simple, and easy to operate.
Owner:安徽省屯溪实验茶场
A flower cutting and seed planting rapid breeding method for flameray gerbera
The present invention discloses the fast germchit propagating method for flameray gerbera cut flower, and aims at providing one kind of fast propagating method of flameray gerbera germchit with less germchit variability, consistent characteristic and robust growth. The method includes cutting the floral receptacle of flameray gerbera bud as the explant, cutting the floral receptacle into small blocks, culturing in inducing culture medium at proper lighting and temperature to grow indefinite bud, culturing the indefinite bud in proliferating culture medium at proper lighting and temperature to grow bush bud, cutting the bush bud into single seedlings, culturing the seedlings in rooting culture medium at proper lighting and temperature to induce rooting, and finally transplanting the rooted seedling in seedling bed. The present invention is applied in propagation of flower.
Owner:广东省珠海市园艺研究所 +1
Intraspecific crossing process of Haliotis asinina
InactiveCN101019518AStrong disease resistanceImprove survival rateAnimal reproductionClimate change adaptationHaliotisHaliotis asinina
The intraspecific crossing process of Haliotis asinina of different geographic colonies includes the procedures of seed selection, promoting mature and induced spawning, insemination, hatching and cultivating. The process is simple and easy to operate, and can obtain Haliotis asinine fry with the excellent characters of its parents. The present invention is suitable for industrial production.
Owner:HAINAN UNIVERSITY
Breeding method for clonal DH33-27 variety of Eucalyptus urophylla*E.grandis
InactiveCN104303993ALarge volume per plantDry straightPlant genotype modificationPhyla lanceolataSmall branch
The invention discloses a breeding method for clonal DH33-27 variety of Eucalyptus urophylla*E.grandis. The method comprises the following steps: firstly, taking Eucalyptus urophylla U16 serving as a female parent and Eucalyptus grandis G33 serving as a male parent as parents; performing emasculation and bagging, pollen collection, hand pollination, cap bag removal, seed collecting and sowing and breeding to realize consistent character expression and stable inheritance. The clonal DH33-27 variety of Eucalyptus urophylla*E.grandis which is not subjected to segregation of character has the variety characteristics that the leaves are lanceolate, terminal buds are green, veins are obvious, seedling stems are slightly red, tree trunks in the early stage is brownish red, the upper parts of the tree trunks are cyan, and the tree trunks are completely peeled in the later period; the clonal DH33-27 variety of Eucalyptus urophylla*E.grandis has the characteristics of high general performance, straight stem shapes, order forest form, small branches, high annual average amount of growing stock and obvious economic benefit, is free of plant diseases and insect pests and suitable for large-scale cultivation promotion.
Owner:广西壮族自治区国有东门林场
Crossbreeding method of mud crab
ActiveCN102860273ASimple methodStrong maneuverabilityClimate change adaptationPisciculture and aquariaGeographic populationStress resistance
The invention provides a crossbreeding method of the mud crab. Inter-species different geographic populations of the mud crab are hybridized and bed to obtain artificial hybrid fry of the mud crab. The crossbreeding method of the mud crab inherits fine traits of both parents and has the advantages that stress resistance is high, breeding survival rate is high and the like. The bred fry of the mud crab have fitting traits and have better stress resistance than the fry of the mud crab in the sea. The bred fry of the mud crab can be widely used for breeding of fine breeds of the mud crab, and growth speed, stress resistance and breeding survival rate of the mud crab are increased. The crossbreeding method of the mud crab is reasonable in design, simple to operate, highly operable and applicable to production.
Owner:ZHEJIANG UNIV
Breeding method for Eucalyptus urophylla*E.grandis clone DH32-43 variety
The invention discloses a breeding method for a Eucalyptus urophylla*E.grandis clone DH32-43 variety. Firstly, a female parent U16(Eucalyptusurophylla) and a male parent G46(Eucalyptusgrandis) are taken as parents; and the Eucalyptus urophylla*E.grandis clone DH32-43 variety which is consistent in phenotypic expression, stable in inheritance and does not have segregation of character is obtained after castration and bagging, pollen collection, artificial pollination, removal and bagging, seed collection and sowing, and breeding. The variety is characterized in that the leaf base of nursery stock is slightly wide in a seedling stage, blades are sharp and long, edges are in the shape of microwaves, terminal buds are dark red, veins are clear and do not protrude, and stems of seedlings are longer than other products in a young seedling stage; and the Eucalyptus urophylla*E.grandis clone DH32-43 variety has the characteristics of straight trunk shape, uniform forest form, small branches, no diseases and insect pests, large annual average amount of growing stock and obvious economic benefit, and is suitable for large-area and wide-range promotion and planting.
Owner:广西壮族自治区国有东门林场
Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines
InactiveCN106416995AAchieving Breeding SuccessEconomic traits are highly organizedPlant genotype modificationSporeHybrid species
The invention discloses a method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines, comprising: hybridizing a cytoplasmic male-sterile line DHCMS08-5-3-6-9 trans-bred with a Brassica oleracea DH line as a female parent and the Brassica oleracea DH line DH05X-1-5-9-8 line as a male parent to prepare DHCMS08-5-5-6-9*DH05X-1-5-9-8 hybrid first-generation combination, named as Qingan 1562. The method of the invention is a method to breed a mid-early maturity novel variety of Brassica oleracea by using two DH lines, a novel hybrid first-generation variety of Brassica oleracea is bred through DH liens with genotype purity of 100% created by culturing isolated microspores of Brassica oleracea, the novel hybrid first-generation variety of Brassica oleracea has highly uniform economic traits and completely exhibited target traits, and the breeding of disease-resistant high-quality high-yield variety breeding targets is achieved.
Owner:NORTHWEST A & F UNIV
Sealwort sterile rooting breeding method in test tube
InactiveCN108476982ANeat and even growthUniform efficacy and qualityHorticulture methodsPlant tissue cultureShootCell budding
The invention relates to a sealwort sterile rooting breeding method in a test tube. The method comprises the following steps: (1) sealwort seed bud selection and sterilization; (2) callus induction; (3) subculture proliferation culture; (4) seedling hardening and rooting; (5) sand bed provisional planting. The sealwort sterile rooting breeding method has the beneficial effects that the sealwort seedling culture period is short; a great number of sealwort seedlings can be formed in five months; the tissue culture cost can be greatly reduced by using multiple shoot proliferation and differentiation; the culture period is shortened; the tissue culture sealwort seedling growth condition is regular and uniform; the medicine effect quality is unified; the breeding speed is high; the influence byexternal environmental factors is avoided; the production can be performed in four seasons in one year; the sealwort tissue culture seedlings have uniform properties; the excellent properties of parents and the growth period consistency can be maintained; the purity of the sealwort tissue culture seedlings is higher; the variation is little; the sealwort field standardized production is facilitated.
Owner:湖北襄草源生态农业科技有限公司
Seed selection method of E. urophylla*E. Grandis clone DH32-13
InactiveCN104285783ALarge volume per plantDry straightPlant genotype modificationPollenEconomic benefits
The invention discloses a seed selection method of E. urophylla*E. Grandis clone DH32-13. The method comprises the following steps: firstly, a female parent Eucalyptus urophylla U16 and a male parent Eucalyptus grandis G46 are taken as parents, and subjected to castration, bagging, pollen collection, artificial pollination, bag removing, seed collecting, seeding and seed selection to obtain the E. urophylla*E. Grandis clone DH32-13 with consistent characters, stable inheritance and not subjected to character segregation. The E. urophylla*E. Grandis clone DH32-13 has the characteristics of straight stem form, neat forest phase, small branches, no plant diseases and insect pests, large annual average stand volume, remarkable economic benefits, and capability of being suitable for being planted in large-area regions.
Owner:广西壮族自治区国有东门林场
Method for producing chilli kernel male sterile plants in large scale
InactiveCN101731146AStable traitsConsistent traitsCultivating equipmentsHorticulture methodsAxillary budBud
The invention provides a method for producing chilli kernel male sterile plants in a large scale. The method comprises the following steps of: inducing an adventitious bud to sprout by a stem section with an axillary bud of a chilli kernel male sterile dual-purpose system sterile plant and forming a plantlet from the adventitious bud; inducing the adventitious bud to sprout by a stem section with an axillary bud of the plantlet and forming a secondary plantnet from the adventitious bud; forming the plantnet through repeatedly subculturing and inducing the axillary bud; forming a large quantity of plantnets through tissue culture; dividing the plantnets to induce and form adventitious roots; and finally, forming a large quantity of chilli kernel male sterile dual-purpose system sterile plants through planting regeneration plants in an open farmland.
Owner:KUNMING UNIV OF SCI & TECH
Tissue culture method for Cymbidium floribundum
ActiveCN102577973ARapid emergenceShort induction timeHorticulture methodsPlant tissue cultureCulture mediumsCymbidium floribundum
The invention relates to a tissue culture method for a plant, in particular to a tissue culture method for Cymbidium floribundum, and belongs to the technical field of agricultural plantation. The tissue culture method for the Cymbidium floribundum comprises the following six steps of: collecting an explant, disinfecting and sterilizing, sowing, proliferating, strengthening seedlings, and performing rooting culture. When the Cymbidium floribundum is propagated through plant tissue culture by a tissue culture technology, the advantage of rapid propagation can be exerted, and a large number of seedlings are obtained in a short time; compared with cottage, the tissue culture method provided by the invention is characterized in that flower colors of plants are seldomly mutated in later culture; and the plant tissue culture serving as a vegetative propagation mode can ensure that the seedlings are regular, facilitates late unified transplantation and plantation management, and can also ensure that various characters of grown product plants and bonsai are consistent. Compared with the conventional propagation mode, the invention has the advantages that a used culture medium is short in induction time, quick in seedling emergence and high in proliferation frequency; and particularly, the seedlings are regular, the method is easy to operate, and the Cymbidium floribundum can be produced on a large scale.
Owner:云南山里红生物科技有限公司
Levo oxiracetam lyophilized powder for injection and preparation method thereof
InactiveCN106692077ANo squirt bottle phenomenonImprove securityOrganic active ingredientsPowder deliveryL serineMANNITOL/SORBITOL
The invention relates to a levo oxiracetam lyophilized powder for injection. The levo oxiracetam lyophilized powder for injection is characterized by being prepared from levo oxiracetam, L-serine, mannitol and sodium glutamate as the raw materials by the steps of concentrated blending, diluted blending, freeze drying and capping. The levo oxiracetam lyophilized powder for injection prepared according to method provided by the invention has the characteristics of fixed shape, no bottle eruption phenomenon in the freeze drying preparation process, good product homogeneity, consistent upper layer and lower layer characters, few impurities, and a total impurity content of less than 0.24%, and is conducive to improving the drug use safety and reducing adverse drug reactions, the product has good stability, and the shelf life is up to 24 months.
Owner:CHONGQING RUNZE PHARM CO LTD
Method for improving African agapanthus embryogenic cell subculture effects
ActiveCN109717080AOptimize the generation systemA large amountPlant tissue cultureHorticulture methodsSomatic embryogenesisBud
The invention provides a method for improving African agapanthus embryogenic cell subculture effects. The method includes the steps: S1 explant selection: taking small African agapanthus buds withoutcracking to disinfect the small buds, taking small stalk explants in a cut manner, and cutting the small stalk explants into small sections; S2 callus induction and subculture: inoculating the small sections of the small stalk explants into callus induction culture media to perform induction culture, and taking calluses with retained small stalk tissues to place the calluses callus subculture media to perform subculture; S3 embryogenic callus induction: taking the calluses without containing retained small stalk tissues cultured and acquired in the step S2 to place the calluses into embryogenic callus induction culture media to perform induction culture; S4 embryogenic cell subculture preservation: taking embryogenic cells cultured and acquired in the step S3 to placing the cells into subculture preservation culture media to perform subculture preservation. According to the method, African agapanthus somatic embryogenesis system is optimized, so that more African agapanthus embryogeniccells with more consistent characters are acquired.
Owner:XINYANG AGRI & FORESTRY UNIV
Method of promoting synchronization rate of germination of somatic embryos of agapanthus africanus
ActiveCN109729979AReduced germinationConsistent traitsHorticulture methodsPlant tissue cultureSomatic embryogenesisOperability
The invention discloses a method of promoting the synchronization rate of germination of somatic embryos of agapanthus africanus. The method comprises the following steps: taking a pedicel explant toinduce a callus; carrying out subculture to induce an embryonic callus; putting the embryonic callus in a mature embryo induction culture medium to be treated at 2-6 DEG C for 2-4 days; and in the conditions of taking different sugars in proportions as a carbon source and paclobutrazol as a growth regulating matter, culturing and inducing mature embryos and germinating seedlings. In an induction method, the quantity of the mature embryos induced by 1g of embryonic cells reaches 958.64 and the synchronization rate is 87.63%. The method is simple in technical treatment and high in operability, solves the problem that the quantity of the somatic embryos is relatively small as a result of long-term subculture process of the embryonic cells and the synchronization rate is relatively low effectively, and reduces the abnormal embryos.
Owner:XINYANG AGRI & FORESTRY UNIV
Sample splitter for sorting soil samples
PendingCN111855364AStable separationUniform traitsPreparing sample for investigationSoil scienceSample preparation
The invention relates to the technical field of soil sample preparation, in particular to a device for separating soil samples. A sample splitter for sorting soil samples is characterized in that a sample splitting device main body is of a circular frame-shaped or square frame-shaped structure, the interior of the sample splitting device main body is divided into four or more than four even numberof symmetrical regions by a grid penetrating through the center of the main body, bottom surfaces of half of the regions are sealed, and the regions with the sealed bottom surfaces are arranged at intervals. The sample splitter realizes uniform separation of the soil samples, the properties of the soil samples and that of powdery samples intercepted in the sample splitting device are kept uniform, consistent and mutually representative by means of the sample splitting device, the separation result meets the national standard, and the preparation phase rate of the screened soil samples is increased.
Owner:北京兰友科技有限公司
Eucalyptus urophylla*grandis clone DH32-26 variety breeding method
InactiveCN104396727ALarge volume per plantDry straightPlant genotype modificationDiseaseEconomic benefits
The invention discloses a eucalyptus urophylla*grandis clone DH32-26 variety breeding method. First, a female parent eucalyptus urophylla U16 and a male parent eucalyptus grandis G46 are adopted as parents; and through the steps of emasculation bagging, pollen collection, artificial pollination, bag removing, seed collection and sowing, and breeding, the eucalyptus urophylla*grandis clone DH32-26 with consistent traits and stable genetic stability, and without trait separation is obtained. The variety has the characteristics of straight trunk, neat forest, small branches, no pest or disease, high average annual accumulation amount, and significant economic benefit. The variety is suitable for large-scale popularization.
Owner:广西壮族自治区国有东门林场
Premature, disease-resistant and stress-resistant mini broccoli breeding method
InactiveCN105145343AImprove fertilityNormal developmentPlant tissue cultureHorticulture methodsSporePollen
The invention discloses a premature, disease-resistant and stress-resistant mini broccoli breeding method and belongs to the technical field of plant cultivation. Flower buds, suitable for microspore culture, of broccoli and cabbage mustard breeding materials from the mid-late uninucleate stage to the early dicaryon stage are used for microspore culture, a large number of regeneration plants are created, excellent DH plants are screened out for selfing, a DH strain stable in botanical character, good in combining ability and large in pollen quantity is obtained, and hybridization first generation seeds F1 are prepared in the flowering stage through artificial emasculationare carried out to obtain; generation-adding is conducted on the F1, then good single plants with botanical characters similar to those of broccoli are selected, selfing in the bud stage is carried out to obtain F2, then generation-adding is conducted on three generations to select self-incompatible strains 11-13-7-8-3 and 12-15-9-3-2 which are excellent and stable and cultivated through continuous selfing in the bud stage, and premature, disease-resistant and stress-resistant mini broccoli is obtained through hybridization. The self-incompatible strains are used for mating, reciprocal cross plants are consistent in character, and the yield of produced seeds is increased by over 50% compared with that of sterile line seed production.
Owner:ZHENJIANG SUIHAN AGRI
Seed selection method of E. urophylla*E. Grandis clone DH30-1
The invention discloses a seed selection method of E. urophylla*E. Grandis clone DH30-1. The method comprises the following steps: firstly, a female parent Eucalyptus urophylla U16 and a male parent Eucalyptus grandis G22 are taken as parents, and subjected to castration, bagging, pollen collection, artificial pollination, bag removing, seed collecting, seeding and seed selection to obtain the E. urophylla*E. Grandis clone DH30-1 with consistent characters, stable inheritance and not subjected to character segregation. The E. urophylla*E. Grandis clone DH30-1 has the characteristics of fast growth, high yield, short rotation (industrial fiber material is adopted), straight stem form, small branches, neat forest phase, good material property, straight texture, good barrenness resistance, and capability of being suitable for being planted in large-area regions.
Owner:广西壮族自治区国有东门林场
Parent propagation method for preventing cabbage parent mixing
InactiveCN104798590AIncrease the number of branches of cabbageIncrease productionVegetative propogationAxillary budPhenotypic trait
Owner:镇江鑫源达园艺科技有限公司
(S)-4-hydroxyl-2-oxo-1-pyrrolidine acetamide freeze-dried powder injection and preparation method thereof
InactiveCN107281124AImprove complianceConsistent traitsOrganic active ingredientsPowder deliveryPatient complianceFreeze-drying
(S)-4-hydroxyl-2-oxo-1-pyrrolidine acetamide freeze-dried powder injection is prepared from (S)-4-hydroxyl-2-oxo-1-pyrrolidine acetamide, L-serine, mannitol, sodium glutamate and benzyl alcohol which serve as raw materials and auxiliary materials through the steps of performing concentrated preparation, performing dilute preparation, freeze-drying and capping, wherein the use amount of the raw materials and the auxiliary materials are as follows: the (S)-4-hydroxyl-2-oxo-1-pyrrolidine acetamide is 36 to 43 percent, the L-serine is 21 to 28 percent, the mannitol is 30 to 35 percent, the sodium glutamate is 4 to 10 percent and the benzyl alcohol is 1 to 3 percent; and the (S)-4-hydroxyl-2-oxo-1-pyrrolidine acetamide freeze-dried powder injection prepared according to the invention has the advantages that the shape is fixed, spray bottle phenomenon is avoided in the freeze-drying preparation process, the product has high uniformity, consistent characters of upper and lower layers and few impurities, the total impurities are less than 0.26 percent, the product has high stability, the shelf life is up to 24 months, the pain of a patient in the injection process is less, and the patient compliance is high.
Owner:CHONGQING RUNZE PHARM CO LTD
Corn cob fermentation substrate and its preparation method and application
ActiveCN107821088BMeet growth requirementsUniform growthCalcareous fertilisersBioloigcal waste fertilisersEconomic benefitsPlantlet
The invention discloses a corn cob fermentation substrate and its preparation method and application. By controlling the physical and chemical properties such as water holding porosity, air porosity and bulk density in the corn cob fermentation process, the fermentation end point is determined, and a cultivation substrate with excellent performance is obtained. . The cultivation substrate has short fermentation time and good physical and chemical properties. The substrate prepared by this method can increase the temperature of the root system of the plant during planting in winter and promote the growth of the plant; at the same time, the invention realizes the resource utilization of corncobs, and has obvious social and economic benefits. benefits and environmental ecological benefits.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
A method of isolated native bulb meristem for rapid proliferation of Amaryllis
InactiveCN105830920BReduce lossHigh degree of developmental synchronyHorticulture methodsPlant tissue cultureGermplasmBud
The invention relates to an in-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs, which belongs to the technical field of rapid proliferation of plants. The method comprises the following steps: germination culture of seeds; proliferation culture of probulbs; and swelling culture of adventitious buds until substantially-swollen small bulbs are obtained. According to the invention, the probulbs obtained after seed germination are used as explants, and proliferation culture and swelling culture are carried out through basal disc cutting of in-vitro bulbs; so the rate of pollution is substantially reduced; loss of wild seedball resources of the Lycoris herbs is decreased; and a great number germplasm populations with a high growth synchronization degree and consistent properties can be efficiently obtained in a short period.
Owner:ZHEJIANG UNIV
A kind of ornamental sunflower seed breeding method
ActiveCN107810858BConsistent traitsHigh purityPlant genotype modificationAngiosperms/flowering plantsLaboratory testSingle plant
The invention discloses an ornamental sunflower elite seed breeding method, which comprises the steps of selecting single plants; selfing and isolating; after a plant laboratory test, extracting a little amount of seeds of finally selected single plants, and carrying out plant-to-row identification; storing a large number of seeds of the finally selected single plants for standby use; after plant-to-row identification, weeding out standby seeds of unhealthy single plants, and mixing and breeding the standby seeds of the single plants with consistent characters in excellent plant-to-row; then selecting the single plants from the plant-to-row with consistent characters, and circulating the operation to obtain the next batch of seeds. According to the method, ornamental sunflower elite seedswith high purity and consistent characters can be quickly and efficiently obtained, the seed reproduction process is shortened, the seed reproduction efficiency is improved, and the seed reproductioncost is saved.
Owner:JIANGSU COASTAL AREA AGRI SCI RES INST
Less-impurity levo oxiracetam sterile powder and preparation method thereof
InactiveCN106692075ASterile powder with drying shrinkageSterile powder with bubbling phenomenonPowder deliveryOrganic active ingredientsL serineFreeze-drying
Less-impurity levo oxiracetam sterile powder is characterized in that the less-impurity levo oxiracetam sterile powder is prepared from levo oxiracetam, L-serine, mannitol, polyethylene glycol and Tween 80 as raw and accessory materials by the steps of concentrated distribution, dilute distribution, freeze drying and capping, the less-impurity levo oxiracetam injection sterile powder prepared by the method has a fixed shape, the process of freeze drying preparation is free of dry shrinkage and bubbling phenomenon, product homogeneity is good, lower layer and upper layer characters are consistent, impurities are less, the total impurities are less than 0.23%, the less-impurity levo oxiracetam sterile powder helps to improve the safety of drug use, and reduces adverse drug reactions, the product has good clarity, the turbidity of the product is lower than that of 0.5 # standard turbidity fluid, and has good stability, and the shelf life is up to 24 months.
Owner:CHONGQING RUNZE PHARM CO LTD
Preparation method of levo-oxiracetam lyophilized powder
InactiveCN107397727AGood product uniformityThe traits of the upper and lower layers are consistentOrganic active ingredientsPowder deliveryPharmaceutical preservativesPhenols
The invention discloses a preparation method of levo-oxiracetam lyophilized powder. The levo-oxiracetam lyophilized powder is prepared from the following raw materials and auxiliary materials in percentage by weight: 50-59% of levo-oxiracetam, 20-25% of L-serine, 10-17% of mannitol, 5-7% of polyethylene glycol 2000, 5-10% of sodium hydrogen sulfite and 0.1-0.5% of phenol; and the levo-oxiracetam lyophilized powder is obtained by carrying out the steps of thick mixing, dilute mixing, freeze drying and capping. The preparation method disclosed by the invention has the advantages that specific excipient combination is utilized, and then a specific freeze drying technology is combined, so that the prepared levo-oxiracetam lyophilized powder has fixed geometry, no phenomena such as dry shrinkage and bubbling are caused in a freeze drying preparation process, product uniformity is good, upper and lower layers are consistent in character, antibacterial property is strong, sterility test is qualified, product stability is good, and insoluble particles are obviously reduced.
Owner:CHONGQING RUNZE PHARM CO LTD
A method for improving the subculture effect of embryogenic cells of Agapanthus
ActiveCN109717080BOptimize the generation systemA large amountHorticulture methodsPlant tissue cultureBiotechnologyEmbryogenesis
The invention provides a method for improving the subculture effect of Agapanthus embryogenic cells, comprising the following steps: S1, taking materials of explants: taking the uncracked small flower buds of Agapanthus abaculata for disinfection treatment, cutting out the explants of small pedicels, and Pedicel explants were cut into small pieces; S2. Induction and subculture of callus: After inoculating small pieces of peduncle explants in callus induction medium for induction culture, callus with residual peduncle tissue was taken Wound tissue is placed in callus subculture medium for subculture; S3, induction of embryogenic callus: take the callus obtained after step S2 culture without residual peduncle tissue and place it in embryogenic callus Induction culture in induction medium; S4, subculture preservation of embryogenic cells: the embryogenic cells obtained after culture in step S3 are placed in subculture preservation medium for subculture preservation. The invention optimizes the somatic embryogenesis system of Agapanthus agapanthus, and obtains agapanthus embryogenic cells with more quantity and more consistent character.
Owner:XINYANG AGRI & FORESTRY UNIV
High-grade bletilla striata tissue culture method
InactiveCN109197591AImprove featuresIncrease productionPlant tissue cultureHorticulture methodsBletilla striataTissue culture
The invention relates to a high-grade bletilla striata tissue culture method particularly including steps of: 1) selection of seeds of high-grade bletilla striata; 2) disinfection; 3) germination reproduction; 4) seedling strengthening culture; 5) rooting culture; 6) acclimatization and seedling hardening; and 7) transplanting. The method guarantees the descendants to be tidy and uniform, is low in mutation rate, can hold the advantageous features and consistent characters of the high-grade plants and can effectively increase yield of the bletilla striata, and has excellent economic value andpromotion value.
Owner:河南云帮农业科技有限公司 +1
A multi-layer composite anti-eccentric wear continuous sucker rod and its preparation device and method
ActiveCN104060944BAccelerated corrosionImprove partial wear resistanceDrilling rodsLayered productsEpoxyManufacturing technology
Owner:SHENGLI OIL FIELD XINDA PIPE IND TECH DEV CO LTD
A method for rapid propagation of air pineapple seedlings through leaf tissue culture
ActiveCN110547196BWide variety of sourcesTroubleshooting Breeding DifficultiesHorticulture methodsPlant tissue cultureTissue cultureReproduction
The invention discloses a method for rapidly propagating air pineapple seedlings through leaf tissue culture, which comprises the following steps: 1) collection of air pineapple leaf explants; 2) disinfection and sterilization of leaf explants; 3) induction of clustered buds of leaf explants; 4 ) Rapid propagation and differentiation culture of sprouts; 5) Rooting culture of strong seedlings; 7) Hardening and transplanting of sterile seedlings. The present invention has the advantages of wide source of materials, fast and convenient material collection, simple operation, high propagation coefficient, fast growth of seedlings, rapid multiplication in a relatively short period of time, and a large number of tissue culture seedlings with consistent traits, consistent growth and robustness, etc., which not only solves the problem of air It is difficult to propagate pineapple seeds, the offspring may mutate, and cannot maintain the excellent traits of the female parent. It also solves the problem that many rare species or varieties will only produce rare daughter plants after flowering, which cannot meet the needs of tissue culture and rapid propagation. Realistic problems of solid materials.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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