A method for improving the subculture effect of embryogenic cells of Agapanthus

A technology of embryogenic cells and Agapanthus, which is applied in the field of cell culture, can solve the problems of failure to take advantage of the somatic embryo system, decrease the uniformity of material development, and coarse embryogenic cell particles, so as to improve the effect of subculture, reduce competition, consistent effect

Active Publication Date: 2020-12-11
XINYANG AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method uses aseptic seedlings as explants, which usually leads to variation of characters and degradation of quality.
At the same time, due to the continuous use of high-concentration exogenous artificially synthesized auxin substance NAA, the synthesis of endogenous IAA was inhibited, resulting in a decrease in the uniformity of material development, thicker embryogenic cell granules, and no loose embryogenic cell mass structure. Lead to fewer embryos, per 1cm 3 Only 21 spherical embryos were obtained from the embryogenic tissue, failing to take advantage of the somatic embryo system in the field of seedling propagation

Method used

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  • A method for improving the subculture effect of embryogenic cells of Agapanthus
  • A method for improving the subculture effect of embryogenic cells of Agapanthus
  • A method for improving the subculture effect of embryogenic cells of Agapanthus

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Embodiment 1

[0047] A kind of method that present embodiment provides to improve the subculture effect of Agapanthus embryogenic cells, concrete steps are as follows:

[0048] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times). Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7 to 1.0 cm;

[0049] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a fla...

Embodiment 2

[0057] A kind of method that present embodiment provides to improve the subculture effect of Agapanthus embryogenic cells, concrete steps are as follows:

[0058] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times). Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7 to 1.0 cm;

[0059] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a fla...

Embodiment 3

[0067] A kind of method that present embodiment provides to improve the subculture effect of Agapanthus embryogenic cells, concrete steps are as follows:

[0068] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times). Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7 to 1.0 cm;

[0069] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a fla...

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Abstract

The invention provides a method for improving the subculture effect of Agapanthus embryogenic cells, comprising the following steps: S1, taking materials of explants: taking the uncracked small flower buds of Agapanthus abaculata for disinfection treatment, cutting out the explants of small pedicels, and Pedicel explants were cut into small pieces; S2. Induction and subculture of callus: After inoculating small pieces of peduncle explants in callus induction medium for induction culture, callus with residual peduncle tissue was taken Wound tissue is placed in callus subculture medium for subculture; S3, induction of embryogenic callus: take the callus obtained after step S2 culture without residual peduncle tissue and place it in embryogenic callus Induction culture in induction medium; S4, subculture preservation of embryogenic cells: the embryogenic cells obtained after culture in step S3 are placed in subculture preservation medium for subculture preservation. The invention optimizes the somatic embryogenesis system of Agapanthus agapanthus, and obtains agapanthus embryogenic cells with more quantity and more consistent character.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a method for improving the subculture effect of Agapanthus embryogenic cells. Background technique [0002] Agapanthus 'Big Blue' (Agapanthus praecox ssp. orientalis 'Big Blue'), also known as "Blue Lily" and "African Lily", is native to southern Africa. It is a monocot perennial herb flower with strong ornamental value. For nearly half a century, Agapanthus has stood out in the development of the international flower industry and has become a popular fresh-cut flower, potted plant and ground cover flower all over the world, and it has shown excellent ornamental value. In addition, Agapanthus is extremely resistant. It can resist high temperatures above 40°C in summer and low temperatures below -10°C in winter. It has no strict requirements on soil and rarely has diseases and insect pests. The field also has huge room for development, and the supply of seedlings is currentl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 岳建华董艳王志勇夏维丽李素青刘文杰
Owner XINYANG AGRI & FORESTRY UNIV
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