A method for improving the subculture effect of embryogenic cells of Agapanthus
A technology of embryogenic cells and Agapanthus, which is applied in the field of cell culture, can solve the problems of failure to take advantage of the somatic embryo system, decrease the uniformity of material development, and coarse embryogenic cell particles, so as to improve the effect of subculture, reduce competition, consistent effect
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Embodiment 1
[0047] A kind of method that present embodiment provides to improve the subculture effect of Agapanthus embryogenic cells, concrete steps are as follows:
[0048] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times). Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7 to 1.0 cm;
[0049] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a fla...
Embodiment 2
[0057] A kind of method that present embodiment provides to improve the subculture effect of Agapanthus embryogenic cells, concrete steps are as follows:
[0058] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times). Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7 to 1.0 cm;
[0059] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a fla...
Embodiment 3
[0067] A kind of method that present embodiment provides to improve the subculture effect of Agapanthus embryogenic cells, concrete steps are as follows:
[0068] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times). Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7 to 1.0 cm;
[0069] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a fla...
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