Sealwort sterile rooting breeding method in test tube

Abstract

The invention relates to a sealwort sterile rooting breeding method in a test tube. The method comprises the following steps: (1) sealwort seed bud selection and sterilization; (2) callus induction; (3) subculture proliferation culture; (4) seedling hardening and rooting; (5) sand bed provisional planting. The sealwort sterile rooting breeding method has the beneficial effects that the sealwort seedling culture period is short; a great number of sealwort seedlings can be formed in five months; the tissue culture cost can be greatly reduced by using multiple shoot proliferation and differentiation; the culture period is shortened; the tissue culture sealwort seedling growth condition is regular and uniform; the medicine effect quality is unified; the breeding speed is high; the influence byexternal environmental factors is avoided; the production can be performed in four seasons in one year; the sealwort tissue culture seedlings have uniform properties; the excellent properties of parents and the growth period consistency can be maintained; the purity of the sealwort tissue culture seedlings is higher; the variation is little; the sealwort field standardized production is facilitated.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, in particular to aseptic rooting and breeding in test tubes, in particular to a method for aseptic rooting and breeding of Polygonatum in test tubes. Background technique [0002] Polygonatum is a plant of the Liliaceae family, which has the functions of invigorating the spleen, nourishing the lungs and promoting body fluid. It is a traditional Chinese medicinal material in my country. As the consumption of Polygonatum increases year by year, the planting area of ​​Polygonatum also increases year by year. At present, the breeding of Polygonatum seedlings is basically the traditional rhizome division breeding and seed breeding. The rhizome division breeding method has low reproduction efficiency, is prone to mildew and rot during the breeding process, and the cost is high; the seed breeding method takes a long time, generally takes one year. It can only germinate into seedlings, and ...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for aseptic rooting and breeding in Polygonatum test tube, so as to solve the problem of low breeding efficiency, easy mildew and rot in the breeding process of the current rhizome division breeding method, and high cost; the live seed breeding method has a long period , it usually takes one year to germinate into seedlings, and the seedlings are of poor purity