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Method of promoting synchronization rate of germination of somatic embryos of agapanthus africanus

A technique for synchronizing and synchronizing the body of Azalea, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of promoting synchronization, strong operability, and reducing the occurrence of deformed embryos.

Active Publication Date: 2019-05-10
XINYANG AGRI & FORESTRY UNIV
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Problems solved by technology

In previous studies on Agapanthus, embryogenic cells were usually used for subculture preservation, and this was used as a platform to conduct research in the fields of gene transformation, gene function research, loss of embryogenicity, and cryopreservation. The optimization of the somatic embryogenesis pathway There are few related reports and studies, especially the research on promoting the synchronization of somatic embryo germination has not been reported

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  • Method of promoting synchronization rate of germination of somatic embryos of agapanthus africanus
  • Method of promoting synchronization rate of germination of somatic embryos of agapanthus africanus
  • Method of promoting synchronization rate of germination of somatic embryos of agapanthus africanus

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Embodiment 1

[0036] This example relates to a method for promoting the synchronization rate of germination of Agapanthus abaculi; it mainly utilizes low-temperature treatment, sugar ratio and hormone regulation to optimize the embryogenesis system of Agapanthus abaculi, and obtain more and more consistent traits lotus embryo. Specific steps are as follows:

[0037] (1) The material of the explants: during the flowering period in May to June, get the uncracked small flower buds of Agapanthus 4 to 5 years old, and carry out disinfection treatment on the aseptic operation table (first use 75% (v / v) ethanol to treat 50 ~70s, with ddH 2 Rinse with O for 3 to 5 times, then disinfect with 5% sodium hypochlorite for 5 to 7 minutes, then ddH 2 Rinse with O for 3 to 5 times, then treat with 75% ethanol for 50 to 70 seconds, and wash with ddH 2 O rinse 3 to 5 times. Drain the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut t...

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Abstract

The invention discloses a method of promoting the synchronization rate of germination of somatic embryos of agapanthus africanus. The method comprises the following steps: taking a pedicel explant toinduce a callus; carrying out subculture to induce an embryonic callus; putting the embryonic callus in a mature embryo induction culture medium to be treated at 2-6 DEG C for 2-4 days; and in the conditions of taking different sugars in proportions as a carbon source and paclobutrazol as a growth regulating matter, culturing and inducing mature embryos and germinating seedlings. In an induction method, the quantity of the mature embryos induced by 1g of embryonic cells reaches 958.64 and the synchronization rate is 87.63%. The method is simple in technical treatment and high in operability, solves the problem that the quantity of the somatic embryos is relatively small as a result of long-term subculture process of the embryonic cells and the synchronization rate is relatively low effectively, and reduces the abnormal embryos.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of Agapanthus lily, and in particular relates to a method for promoting the synchronization rate of germination of agapanthus somatic embryos. Background technique [0002] Agapanthus 'Big Blue' (Agapanthus praecox ssp. orientalis 'Big Blue'), also known as "Blue Lily" and "African Lily", is native to southern Africa. It is a monocot perennial herb flower with strong ornamental value. For nearly half a century, Agapanthus has stood out in the development of the international flower industry and has become a popular fresh-cut flower, potted plant and ground cover flower all over the world, and it has shown excellent ornamental value. In addition, Agapanthus is extremely resistant. It can resist high temperatures above 40°C in summer and low temperatures below -10°C in winter. It has no strict requirements on soil and rarely has diseases and insect pests. The field also has huge room for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 岳建华董艳腾云王亚莉张华张梦帅史亚冰
Owner XINYANG AGRI & FORESTRY UNIV
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