The invention discloses a high-efficiency breeding method for whitebait triploids, comprising the following steps: preparation of tamoxifen inhibitors, preparation of tamoxifen injection, injection treatment of female parents, cold shock + hydrostatic pressure treatment, female Preparation of hormone inhibitor solution, taking fertilized eggs and putting them in the estrogen inhibitor solution, obtaining the pure line of whitebait male tetraploid, taking the male tetraploid as the male parent, taking the common diploid as the female parent, and adopting backcross way to obtain 100% triploid. By adopting the method of the present invention, 100% whitebait triploid pure lines can be obtained, which improves the breeding rate and emergence rate of triploids, reduces the trouble of breeding and selection, and is more important for the germplasm and germplasm of natural aquatic products. The ecological environment is pollution-free.