Method for increasing breeding rate of male tetraploids of crucian carps and carps

A male and female technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, diseases, etc., can solve problems such as difficulty in obtaining a quadruple system of purity, difficulty in obtaining a quadruple system, biological hazards, etc., and achieve improved breeding rate and emergence rate, high purity, and the effect of inhibiting androgenic

Inactive Publication Date: 2016-11-16
HUNAN UNIV OF ARTS & SCI
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  • Abstract
  • Description
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Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle
In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

Method used

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  • Method for increasing breeding rate of male tetraploids of crucian carps and carps

Examples

Experimental program
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Effect test

Embodiment 1

[0016] The method for improving the male tetraploidy success rate of crucian carp comprises the following steps:

[0017] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 90mg / ml, and wait for use;

[0018] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 240ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is about 0.375mg / ml, stand-by;

[0019] (3) Injection treatment of the female parent: before the artificial egg laying of the female parent, artificially inject tamoxifen injection into the female parent according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 80 μg / kg;

[0020] (4) Cold shock + h...

Embodiment 2

[0024] The method for improving the male carp tetraploidy success rate comprises the following steps:

[0025] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 90mg / ml, and wait for use;

[0026] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 450ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is 0.2mg / ml, stand-by;

[0027] (3) Injection treatment of the female parent: before the artificial egg laying of the female parent, artificially inject tamoxifen injection into the female parent according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 120 μg / kg;

[0028] (4) Cold shock + hydrostatic pressur...

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Abstract

The invention discloses a method for increasing the breeding rate of male tetraploids of crucian carps and carps. The method comprises the following steps of preparing of a tamoxifen inhibitor, preparing of a tamoxifen injection, injection treating of female parents, cold shock and hydrostatic pressure treating, preparing of an estrogen inhibitor solution and obtaining of a pure tetraploid line. According to the method, a 100% pure male tetraploid line of the crucian carps and the carps can be obtained, the breeding rate and the fry obtaining rate of the tetraploids are effectively increased, the male tetraploid fish line with the higher purity and stable progenies can be obtained compared with hybridization, and a firm foundation is laid for breeding of sterile triploid crucian carps and carps.

Description

technical field [0001] The invention relates to a tetraploid breeding method for crucian carp and carp, in particular to a method for increasing the male tetraploid success rate of crucian carp and carp. Background technique [0002] Crucian carp: refers to diploid crucian carp (including green crucian carp, red crucian carp, white crucian carp and black crucian carp, etc.); It grows fast and has delicious meat. The artificial triploid crucian carp (natural triploid crucian carp is fertile) and the sterility characteristics of common carp make it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, sterile triploid fish are obtained by crossing between tetraploid fish and diploid fish, but to obtain more than 98% of tetraploid fish, it is necessary to establish genetically stable and extremely high-purity Tetraploid fish. [0003] In fish genetics and breeding, cold / heat shock is usually used,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61K38/24A61K31/568A61P15/08A61K31/138A61K31/135
CPCA01K61/00A61K31/135A61K31/138A61K31/568A61K38/24A61K2300/00Y02A40/81
Inventor 杨品红刘良国张运生
Owner HUNAN UNIV OF ARTS & SCI
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