Corbicula fluminea male tetraploid pure line efficient breeding method

A tetraploid and river clam technology is applied in fish farming, application, climate change adaptation, etc. It can solve the problems of high-efficiency breeding of tetraploid all-male river clams, reduce the time process of cold shock and reduce operation Difficulty, inhibition of androgenic effect

Inactive Publication Date: 2016-11-23
HUNAN UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the genetic breeding of river clams, so far there has been

Method used

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  • Corbicula fluminea male tetraploid pure line efficient breeding method

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Example Embodiment

[0014] Example one

[0015] The efficient breeding method of tetraploid male tetraploid of river clam includes the following steps:

[0016] (1) Preparation of tamoxifen feeding material: take tamoxifen powder with a purity of more than 95% and add it to the artificial feeding feed of river clams to make feeding material, in which the content of tamoxifen is 15g / 500g feeding Material, to be used;

[0017] (2) Feeding: 5 days before the female clams lay eggs, the female clams are fed with tamoxifen feeding according to the conventional feeding method, twice a day, at 8:00 in the morning, 10g per 100g female clams are fed. Feed 10g per 100g female clams at 6:00 pm; until the eggs mature, then take the male clams together to fertilize them naturally, and get fertilized eggs for use;

[0018] (3) Cold shock + hydrostatic pressure treatment: 3 to 5 minutes before a cleavage, using a combination of cold shock and hydrostatic pressure, put the fertilized eggs in step (2) into cold water at ...

Example Embodiment

[0022] Example two

[0023] The efficient breeding method of tetraploid male tetraploid of river clam includes the following steps:

[0024] (1) Preparation of tamoxifen feeding material: take tamoxifen powder with a purity of more than 95% and add it to the artificial feeding feed of river clams to make feeding material, wherein the tamoxifen content is 20g / 500g feeding Material, to be used;

[0025] (2) Feeding: 10 days before the female clams lay eggs, the female clams will be fed with tamoxifen according to the conventional feeding method, twice a day, 20g per 100g female clams will be fed at 8:00 in the morning. Feed 15g per 100g female clams at 6:00 pm; until the eggs mature, then take the male clams together to fertilize them naturally, and get fertilized eggs for use;

[0026] (3) Cold shock + hydrostatic pressure treatment: During a cleavage, the method of combining cold shock and hydrostatic pressure is used to put the fertilized egg in step (2) into cold water at a tempera...

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Abstract

The invention discloses a corbicula fluminea male tetraploid pure line efficient breeding method. The method comprises the steps that tamoxifen feed is prepared, female corbicula fluminea is fed with the tamoxifen feed, cold shock and hydrostatic pressure treatment are carried out, an estrogen inhibitor solution is prepared, soaking in an estrogen inhibitor solution is carried out, and a male corbicula fluminea tetraploid pure line is obtained. By means of the method, the 100% corbicula fluminea male tetraploid pure line can be obtained. Corbicula fluminea tetraploid is effectively bred, the tetraploid bred rate and incubation rate are improved, and a solid foundation is laid for production of triploid sterile fish.

Description

technical field [0001] The invention relates to a polyploid breeding method of river clams, in particular to a method for efficiently breeding male tetraploid pure lines of river clams. Background technique [0002] River clams, commonly known as river clams, yellow clams, etc., belong to the class of bivalves and are widely distributed in the waters of all continents except Antarctica. The shell is thick and firm, round or nearly triangular in shape. Eating it can nourish the liver, and it is also a bait for fish and poultry, and can be used as a fertilizer for farmland. River clams reach sexual maturity at the age of 3 months (shell length 1.1-1.2cm), dioecious (occasionally hermaphrodite), mature in batches, lay eggs in batches, and fertilize in vitro. Because of the sterility characteristics of the triploid clam, it shows more significant advantages in terms of growth speed, disease resistance, and meat quality. In the prior art, the quickest and most effective way to...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨品红邹万生韩庆
Owner HUNAN UNIV OF ARTS & SCI
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