Efficient breeding method for siniperca chuatsi male tetraploid pure line

Abstract

The invention discloses an efficient breeding method for a siniperca chuatsi male tetraploid pure line. The method includes the following steps that a tamoxifen inhibitor is prepared, a tamoxifen injection is prepared, a female parent is treated through injection, cold shock and hydrostatic pressure treatment is carried out, an estrogen inhibitor solution is prepared, and the siniperca chuatsi male tetraploid pure line is obtained. By means of the method, the 100% siniperca chuatsi male tetraploid pure line can be obtained, and the breeding rate and emerging rate of tetraploid are effectively increased; compared with hybridization, the fish male tetraploid line with higher purity and more stable offspring can be obtained, and a solid foundation is laid for breeding of sterile triploid siniperca chuatsi.

Description

technical field [0001] The invention relates to a polyploid breeding method for mandarin fish, in particular to a method for efficiently breeding male tetraploid pure lines of mandarin fish. Background technique [0002] Mandarin fish is further divided into cocked-mouthed mandarin fish, big-eyed mandarin fish, and spotted mandarin fish. The side of the body is flat, the back is raised, the body is thicker, and the head is pointed. The meat is tender, with less thorns and more meat. The meat is petal-shaped and delicious. It is really the best fish. The sterility characteristic of triploid mandarin fish makes it show more significant advantages in growth speed, stress resistance, disease resistance and meat quality. In the prior art, the sterile triploid mandarin fish is obtained by hybridization between the tetraploid mandarin fish and the diploid mandarin fish, but to obtain more than 98% of the triploid fish, then it is necessary to establish genetic stability and purity...

AI Technical Summary

Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle

In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

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