Method for increasing breeding rate of male culter tetraploids

Abstract

The invention discloses a method for increasing the breeding rate of male culter tetraploids. The method comprises the following steps: preparation of a tamoxifen inhibitor; preparation of a tamoxifen injection; injection treatment of a female parent; cold shock and hydrostatic pressure treatment; preparation of an estrogen inhibitor solution; obtaining of a male culter tetraploid pure line. With adoption of the method, the male culter tetraploid pure line with the purity being 100% can be obtained, the breeding rate and the hatching rate of the tetraploids are increased, trouble in breeding and seed selection is reduced, and a solid foundation is laid for culturing sterile triploid pure lines which are pollution-free for germplasm of natural aquatic species and the ecological environment.

Description

technical field [0001] The invention relates to a method for cultivating tetraploids of carp, in particular to a method for improving the male tetraploid success rate of carp. Background technique [0002] Crawfish, there are cichlids, Mongolian cichlids, etc. The back and upper part of the body are light gray, the abdomen is silvery white, and the lower jaw is thick and protruding upwards. The body is small, but the meat is fresh and tender, and has high nutritional value. The sterility characteristic of triploid chrysalis makes it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, sterile triploid fish are obtained by crossing between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, it is necessary to establish genetically stable and extremely high-purity Tetraploid fish. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as...

AI Technical Summary

Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle

In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

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