Method for increasing breeding rate of male culter tetraploids

A technology of male and carp fish, applied in the direction of organic active ingredients, medical preparations containing active ingredients, diseases, etc., can solve problems such as difficulty in obtaining a quadruple system of purity, difficulty in obtaining a quadruple system, biological hazards, etc., and achieve improved Raising rate and emergence rate, improving survival rate, inhibiting male-promoting effect

Inactive Publication Date: 2016-11-23
HUNAN UNIV OF ARTS & SCI
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Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle
In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

Method used

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  • Method for increasing breeding rate of male culter tetraploids

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Effect test

Embodiment 1

[0016] The method for improving the male quadruple growth rate of C. chinensis comprises the following steps:

[0017] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 60mg / ml. use;

[0018] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 240ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is about 0.25mg / ml, stand-by;

[0019] (3) Injection treatment of the female parent: Before the female parent artificially lays eggs, the female parent is artificially injected with tamoxifen injection according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 120 μg / kg, that is, Inject 120 μg of tamoxifen injectio...

Embodiment 2

[0024] The method for improving the male tetraploidy success rate of C. mongolicus comprises the following steps:

[0025] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 120mg / ml, and wait for use;

[0026] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 400ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is 0.3mg / ml, stand-by;

[0027] (3) Injection treatment of the female parent: Before the female parent artificially lays eggs, the female parent is artificially injected with tamoxifen injection according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 160 μg / kg, that is, Inject 160 μg of tamoxif...

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Abstract

The invention discloses a method for increasing the breeding rate of male culter tetraploids. The method comprises the following steps: preparation of a tamoxifen inhibitor; preparation of a tamoxifen injection; injection treatment of a female parent; cold shock and hydrostatic pressure treatment; preparation of an estrogen inhibitor solution; obtaining of a male culter tetraploid pure line. With adoption of the method, the male culter tetraploid pure line with the purity being 100% can be obtained, the breeding rate and the hatching rate of the tetraploids are increased, trouble in breeding and seed selection is reduced, and a solid foundation is laid for culturing sterile triploid pure lines which are pollution-free for germplasm of natural aquatic species and the ecological environment.

Description

technical field [0001] The invention relates to a method for cultivating tetraploids of carp, in particular to a method for improving the male tetraploid success rate of carp. Background technique [0002] Crawfish, there are cichlids, Mongolian cichlids, etc. The back and upper part of the body are light gray, the abdomen is silvery white, and the lower jaw is thick and protruding upwards. The body is small, but the meat is fresh and tender, and has high nutritional value. The sterility characteristic of triploid chrysalis makes it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, sterile triploid fish are obtained by crossing between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, it is necessary to establish genetically stable and extremely high-purity Tetraploid fish. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61K38/24A61K31/568A61P15/08A61K31/138A61K31/135
CPCA01K61/00A61K31/135A61K31/138A61K31/568A61K38/24A61K2300/00Y02A40/81
Inventor 杨品红罗玉双刘飞
Owner HUNAN UNIV OF ARTS & SCI
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