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Efficient breeding method of freshwater mussel triploid pure line

A triploid and river mussel technology, applied in fish farming, application, climate change adaptation, etc., can solve the problem of backcrossing between all-male tetraploids and diploid females, which can improve the breeding rate and The effect of seedling emergence rate, improving survival rate and reducing trouble

Inactive Publication Date: 2016-12-07
HUNAN UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, so far, there has not been a method for producing triploid mussels by this method, let alone a precedent for backcrossing an all-male tetraploid with a diploid female parent

Method used

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  • Efficient breeding method of freshwater mussel triploid pure line

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The high-efficiency breeding method of triploid pure line of mussels comprises the following steps:

[0017] (1) Preparation of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95% and add it to the artificial breeding feed of river mussels to make a feeding material, in which the content of tamoxifen is 10g / 500g feed ,stand-by;

[0018] (2) Feeding: 5 days before the oviposition of the female mussel, the female mussel was fed tamoxifen feeding material according to the conventional feed feeding method, twice a day, 10g per 100g of the female mussel was fed at 8:00 in the morning, At 6:00 p.m., feed 10g per 100g of female mussels; until the eggs mature, take the male mussels and hang them together to make them naturally spawn and fertilize, and fertilize the "egg-bearing" female mussels;

[0019] (3) Heat shock treatment: Observe the development of the fertilized eggs of the "pregnant" female mussels. 3 minutes before the first cleavage of t...

Embodiment 2

[0025] The high-efficiency breeding method of triploid pure line of mussels comprises the following steps:

[0026] (1) Preparation of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95% and add it to the artificial breeding feed of river mussels to make a feeding material, in which the content of tamoxifen is 20g / 500g feed ,stand-by;

[0027] (2) Feeding: 10 days before the oviposition of the female mussel, the female mussel was fed with tamoxifen feeding material according to the conventional feed feeding method, twice a day, 5g per 100g of the female mussel at 8:00 in the morning, At 6:00 p.m., feed 8g per 100g of female mussels; until the eggs mature, take the male mussels and hang them together to make them naturally lay eggs and fertilize them, and fertilize the "egg-bearing" female mussels;

[0028] (3) Heat shock treatment: Observe the development of the fertilized eggs of the "pregnant" female mussels. 2 minutes before the first cleavage ...

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Abstract

The invention discloses an efficient breeding method of a freshwater mussel triploid pure line. The method comprises the following steps of preparing a tamoxifen feeding material, feeding the tamoxifen feeding material to female freshwater mussels, conducting heat shock treatment, preparing an estrogen inhibitor solution, conducting estrogen inhibitor solution bathing, obtaining a male tetraploid pure line of the freshwater mussels and obtaining triploid freshwater mussels in a backcross mode. By means of the method, the 100% freshwater mussel triploid pure line can be obtained, the bred rate and fry emergence rate of the triploid freshwater mussels are increased, troubles of strain breeding and strain selecting are reduced, and more importantly, the method is free of pollution to germplasm of natural aquatic species and the ecological environment.

Description

technical field [0001] The invention relates to a polyploid breeding method of river mussels, in particular to a method for efficiently breeding triploid pure lines of river mussels. Background technique [0002] River mussels, also known as Hewai, river clams, bird shells, Xishi tongue, etc., belong to the mollusk Phylumbranchidae, and are shellfish aquatic animals that feed on algae. There are dozens of species of common horned-backed mussels, pleated crested mussels, triangular sail mussels, and clams, which are produced in rivers and lakes in most parts of my country. The meat of river mussels is particularly crisp, tender and delicious. It is a delicacy for banquets and a good material for artificial pearl breeding. For the reproduction of mussels, the mature eggs of female clams are discharged into the mantle cavity of the body through the genital opening; the mature sperm of the male clam are discharged from the vas deferens through the genital opening to the upper g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨品红罗玉双刘飞
Owner HUNAN UNIV OF ARTS & SCI
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