Efficient breeding method for spiral shell male tetraploid pure line

A tetraploid, snail male technology, applied in the field of high-efficiency breeding of snail male tetraploid pure line, can solve the problem of no reports of tetraploid all-male snail breeding, and achieves inhibition of DNA division, inhibition of male promotion, reduction of adverse effects

Inactive Publication Date: 2016-10-12
HUNAN UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the genetic breeding of snails, there has been no report on the breeding of tetraploid all male snails so far.

Method used

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  • Efficient breeding method for spiral shell male tetraploid pure line

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A high-efficiency breeding method for male tetraploid pure lines of snails comprises the following steps:

[0016] (1) Preparation of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95% and add it to snail artificial feeding feed to make feeding material, in which the content of tamoxifen is 10g / 500g feeding material ,stand-by;

[0017] (2) Feeding: 5 days before the female snail spawns, the female snail is fed with tamoxifen feeding material according to the conventional feed feeding method, twice a day, 10g per 100g female snail is fed at 8:00 in the morning, At 6:00 p.m., feed 8g per 100g of female snails; until the eggs mature, then take the male snails and put them together to make them naturally spawn and fertilize, and fertilized female snails will be obtained;

[0018] (3) Heat shock treatment: Observe the development of the fertilized eggs of the pregnant snails. 2 minutes before the first cleavage of the fertilized eggs, put the a...

Embodiment 2

[0023] A high-efficiency breeding method for male tetraploid pure lines of snails comprises the following steps:

[0024] (1) Preparation of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95%, add it to the snail artificial feeding feed to make feeding material, and the tamoxifen content is 20g / 500g feeding material ,stand-by;

[0025] (2) Feeding: 10 days before the female snail spawns, the female snail is fed with tamoxifen feeding material according to the conventional feed feeding method, twice a day, at 8:00 in the morning, every 100g female snail is fed with 5~ 5g, 10g per 100g of female snails at 6:00 pm; until the eggs mature, then take the male snails and put them together to make them naturally spawn and fertilize, and fertilized egg-bearing female snails are obtained;

[0026] (3) Heat shock treatment: Observe the development of the fertilized eggs of the fertilized female snails. 3 minutes before the first cleavage of the fertilized e...

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Abstract

The invention discloses an efficient breeding method for a spiral shell male tetraploid pure line. The method comprises the steps of tamoxifen feed preparation, tamoxifen feed putting for feeding female spiral shells, heat shock treatment, estrogen inhibitor solution preparation, estrogen inhibitor solution bathing, and obtaining of the spiral shell male tetraploid pure line. By means of the method, the 100% spiral shell male tetraploid pure line can be obtained. By means of the method, the spiral shell tetraploid can be effectively bred, the breeding rate and the fry rate of the tetraploid are increased, and a firm base is laid for producing triploid sterile fishes.

Description

technical field [0001] The invention relates to a polyploid breeding method for snails, in particular to a method for efficiently breeding male tetraploid pure lines of snails. Background technique [0002] The distribution and variety of snails refer to gastropod animals, especially gastropod animals that have a closed shell that can be completely retracted into it for protection. Common ones include ring-shaped snails, field snails, etc. , The breeding method of snails is generally ovoviviparous. Due to the sterile characteristics of the snail triploid, it shows more significant advantages in terms of growth speed, disease resistance, and meat quality. In the prior art, to obtain triploid sterile snails, the quickest and most effective method is to obtain tetraploid snails by crossing diploid snails with diploid snails. Then it is necessary to establish a tetraploid snail with stable genetics and high purity. Pure line. However, in the genetic breeding of snails, so far...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 杨品红黄海洪类延菊
Owner HUNAN UNIV OF ARTS & SCI
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