35results about How to "Effective breeding" patented technology

A method for defining the selective index used for the multi-character breeding of Chinese prawn includes such steps as using BLUP method to calculate the breeding value of each character for the individual or family, standardizing each breeding value, percentage weighting to the importance of each character in breeding target, and multiplying said standardized breeding value with the percentage weighted value to obtain said selective index used to choose individual or family.

The invention discloses a pure line breeding method for increasing the local fowl breed egg yield. The method includes the following steps that (1) the number of eggs laid by individual hens ranging from 21-week-old to 32-week-old, the number of eggs laid by individual hens in the egg laying peak week and the number of weeks of the egg laying peak weeks of all the individual hens are detected and determined; (2) parameter indexes of reserved eggs for breeding are stipulated; the number of the eggs laid by the selected individual breeding hens ranging from 21-week-old to 32-week-old is larger than or equal to 42, the number of the eggs laid by all the individual hens in the egg laying peak week is larger than or equal to 5, and the number of the weeks of the egg laying peak weeks of all the individual hens is larger than or equal to 1, and the number is increased generation by generation; (3) breeding cocks and breeding hens are reserved and mated to form new families, the cocks and the hens which are of the homologous series but not near of kin are selected to form new families, the proportion of the cocks and the hens is 1:10-16, and over forty families form a pure line to breed the next generation of breeding chicken; step (2) and step (3) are carried out repeatedly till over three generations are bred. By means of the method, the egg yield of local chicken can be increased effectively and quickly, the variety breeding effect is remarkable for the local chicken with the low egg yield, the egg yield of breeding chicken is increased over 40% after four generations of breeding, and the generation breeding cycle is advanced by over 10 weeks.

The invention discloses a dairy cow milk progesterone colloidal gold test strip and a preparation method thereof. The preparation method of the dairy cow milk progesterone colloidal gold test strip comprises the steps of preparation of main solutions, namely preparing a trisodium citrate solution, preparing a chloroauric acid solution, preparing a K2CO3 solution, preparing a PBS, a BSA (Bovine Serum Albumin) solution, preparing a sample pad treating fluid and preparing a gold mark combination solution; and preparation and authentication of colloidal gold, namely preparing colloidal gold, authenticating the quality of colloidal gold, preparing and purifying a gold marked progesterone antibody compound, selecting an antigen coating solution and a gold mark diluting solution, selecting gold marked antibody diluent and dilution, selecting an NC film and secondary antibody coating concentration, selecting an NC film blocking liquid and blocking time, selecting the sample pad, and assembling the colloid gold test strip. The test strip disclosed by the invention has a good stable effect, is good in stability and high in flexibility and sensitivity, is in favor of clinical application and the guidance of clinical hybridization work, and is capable of effectively guiding a worker to diagnose and treat diseases, and the pregnancy rate is improved.

The invention provides a production method of rapid propagation nutritional paper for sprouting vegetables. The production method comprises the following steps: (1) grinding tea seed cake, evenly mixing the ground tea seed cake with a 1% EM stock solution according to a mass ratio of (92-94):(8-6), adding clear water, adjusting water content to be 60-65%, and carrying out aerobic fermentation for 15-20 days to prepare a fermented material; (2) evenly mixing the fermented material prepared in step 1 with the 1% EM stock solution according to a mass ratio of 1:8, arranging the mixture into a fermentation tank for anaerobic fermentation for 3-5 days, and filtering to obtain nutritional fermented liquid; (3) adding zeolite powder into the nutritional fermented liquid, evenly mixing, arranging a hydrophilic non-woven fabric into a container which contains the nutritional fermented liquid, soaking for 10-12 hours, fully mixing 3-4 times during soaking, taking out the hydrophilic non-woven fabric which is soaked in the nutritional fermented liquid and adsorbed with the zeolite powder simultaneously, and drying to finally prepare the rapid propagation nutritional paper for sprouting vegetables. The sprouting vegetables produced by utilizing the nutritional paper provided by the invention meet green standards, and according to the production method, the production cost is low, and output and benefits are high.

The invention discloses a rice breeding method capable of relieving heavy metal cadmium pollution. According to the rice breeding method, low-cadmium rice is cultivated by using self gene differences of the rice and rice varieties with low cadmium accumulation amount can be effectively bred under the conditions of long soil and water body treatment period and high cost; the content of heavy metal in the rice is reduced and the healthy, environment-friendly and green requirements are met. According to the rice breeding method, the cultivated low-cadmium rice can effectively reduce the intake of the heavy metal of human beings so as to promote the health of human bodies.

The invention relates to a semi-lignified twig cutting and fast root taking and seedling growing method for patchouli. The method comprises the key links of scion-seed stock plant selection, scion picking, root speeding treatment, cutting medium preparation, cutting and cutting post-management. According to the method, the cutting seedlings of the patchouli can root in advance, roots can be taken fast, the cutting survival rate can reach more than 99 percent, sprouting only needs 15 days, and cutting and seedling growing can be carried out all the year round. The method is easy to operate and popularize, a large number of high-quality patchouli seedlings can be cultured in a short time, and a good foundation is laid for the development of the patchouli industry.

The invention discloses a multi-functional nursing bed and a manufacturing method thereof. Inserting holes capable of allowing telescopic supporting rods to be installed therein are formed in the two sides and the middle of a base plate, connecting bands are manufactured between two rows of the telescopic supporting rods of the two sides of the base plate, pressure sensors are manufactured on the telescopic supporting rods, and the connecting bands between the two rows of the telescopic supporting rods of the two sides of the base plate form a bed surface capable of allowing a patient to lie down. Through the control over the telescopic supporting rods to go up and down, the connecting bands are driven to go up and down, since each supporting rod goes up and down section by section, a cyclic fluctuation state is formed, each pressed portion of the body lying on the connecting bands can be changed, a stress-less scope can be retained for the portion where the pressure sore occurs through the disassembly of the connecting bands, the body turnover is helped through the control over the telescopic supporting rods to go up at one side and go down at the other side, when sanitation cleaning is needed and the patient needs to defecate, the operation by a single person can be conducted conveniently, a prone position can be changed into a sitting position, the single position in bed can be changed according to needs, the nursing needs of the patient are effectively improved, and the manpower resources are effectively reduced.

The invention provides anti-bacterial and mould-proof anion diatom ooze dry powder, which relates to the field of building and ornament materials. Diatom ooze is a novel environmental-protection decorative material and has the functions on adsorbing formaldehyde and adjusting humidity, but on the current market, most of diatom ooze dry powder is unreasonable in formula, and does not have anti-microbial, mould proof and anion releasing functions, and aiming at the defects of the prior art, the invention relates to the anti-bacterial and mould-proof anion diatom ooze dry powder, which is characterized by being prepared from the following raw materials in parts by mass: 30 to 45 parts of kieselguhr, 15 to 30 parts of bentonite, 15 to 30 parts of ash calcium powder, 15 to 30 parts of heavy calcium carbonate powder, 2 to 8 parts of titanium dioxide, 5 to 10 parts of quartz powder, 8 to 15 parts of anion powder, 6 to 15 parts of redispersible powder, 4 to 10 part of xanthan rubber powder, 3to 6 parts of white cement, 2 to 6 parts of polypropylene staple fiber, 0.5 to 1.5 parts of quaternary ammonium salt, 0.3 to 1 part of sodium bicarbonate, 0.2 to 1.2 parts of sorbic acid, 0.2 to 1.2 parts of copper sulfate, 0.1 to 1 part of sodium fluoride, 0.5 to 2 parts of OBPA, 0.8 to 2.5 parts of diethanol amine, 0.05 to 0.7 part of dispersing agent, and 0.03 to 0.5 part of lubricating agent.

The invention discloses an antibacterial negative ion coating. The antibacterial negative ion coating comprises the following components in parts by mass: 0 to 45 parts of aqueous color paste, 40 to 85 parts of an epoxy resin emulsion, 0.2 to 10 parts of a thickener, 0 to 2 parts of a film forming auxiliary agent, 0 to 3 parts of propylene glycol, 5 to 20 parts of an epoxy curing agent, 1 to 3 parts of nano silver, 1 to 5 parts of a dispersing aid, 0.2 to 5 parts of tourmaline, 0.2 to 5 parts of seagull stone, 5 to 15 parts of silica gel salt, 4 to 20 parts of negative ion powder, and 2 to 30parts of heavy calcium powder. The antibacterial negative ion coating has a reasonable formula, the preparation method is simple, the antibacterial negative ion coating can realize effective sterilization, and has certain odor-adsorption function, various functions and obvious effects.

The invention discloses a dairy cow milk progesterone colloidal gold test strip and a preparation method thereof. The preparation method of the dairy cow milk progesterone colloidal gold test strip comprises the steps of preparation of main solutions, namely preparing a trisodium citrate solution, preparing a chloroauric acid solution, preparing a K2CO3 solution, preparing a PBS, a BSA (Bovine Serum Albumin) solution, preparing a sample pad treating fluid and preparing a gold mark combination solution; and preparation and authentication of colloidal gold, namely preparing colloidal gold, authenticating the quality of colloidal gold, preparing and purifying a gold marked progesterone antibody compound, selecting an antigen coating solution and a gold mark diluting solution, selecting gold marked antibody diluent and dilution, selecting an NC film and secondary antibody coating concentration, selecting an NC film blocking liquid and blocking time, selecting the sample pad, and assembling the colloid gold test strip. The test strip disclosed by the invention has a good stable effect, is good in stability and high in flexibility and sensitivity, is in favor of clinical application and the guidance of clinical hybridization work, and is capable of effectively guiding a worker to diagnose and treat diseases, and the pregnancy rate is improved.

The invention relates to a solvent-free type heavy anti-corrosion coating based on a secondary doped polyaniline nanometer material and belongs to the technical field of heavy anti-corrosion coating. The solvent-free type heavy anti-corrosion coating is characterized by comprising a component A and a component B; the weight ratio of the component A to the component B is 10:(2.6-3.2); the component A comprises the following components by weight percent: 32-40% of liquid epoxy resin, 7-18% of a nuclear shell rubber flexibilizer, 6-10% of a reactive diluent, 0.6-1.4% of a silane coupling agent, 1.2-2.5% of a dispersing agent, 0.3-0.7% of a de-foaming agent, 1.5-2.4% of a sterilizing agent, 0.6-1.2% of a thixotropic agent, 1.6-2.5% of a flatting agent, 4-12% of zinc phosphate, 4-8% of mica powder, 3.5-20% of superfine talcum powder and 7-12% of titanium dioxide, wherein the sum of the weight percent of the components is 100%; the component B comprises the following components by weight percent: 44-56% of phenolic amine, 20-44% of modified polyamide, 1.6-2.4% of a dispersing agent, 0.2-0.6% of a de-foaming agent, 2-6% of nanometer graphite powder, 7-15% of polyaniline and 0.4-0.8% of a thixotropic agent, wherein the sum of the weight percent of the components is 100%. The anti-corrosion coating provided by the invention is environmentally friendly and nontoxic and has the advantages of excellent mechanical properties, low surface processing requirement, applicability on wet and even water-containing metal substrate, excellent mold-proof and antibacterial properties, excellent corrosion protection and corrosion inhibition, high site construction operability, and the like.

The invention provides an SNP marker associated with bacterial septicemia of schizothorax prenanti and application of the SNP marker. A nucleotide sequence of the SNP marker is shown in SEQ ID NO. 1; a 779th base of the sequence from 5' terminus is C or T. At an SNP site of the invention, the probability that the schizothorax prenanti having genotype CT is infected with bacterial septicemia is significantly higher than that of homozygous CC type individuals. Therefore, whether the schizothorax prenanti is easily infected with bacterial septicemia can be determined effectively by detecting the SNP of the schizothorax prenanti. The CT genotype individuals are knocked out in breeding of parents; and the marker of the invention is used for auxiliary breeding to accelerate a cultivation process of excellent disease-resistant varieties of the schizothorax prenanti.

The invention provides a composite water purification material preparation method. The steps are as follows: (1) KDF, limonite, tourmaline and mesoporous composite molecular sieve are mixed accordingto a certain proportion, added with water and stirred for 20 to 30 minutes; (2) the mixed material is homogenized for 30 to 40 minutes, added with clay and uniformly stirred again; (3) the material obtained in step 2 is dewatered to be concentrated and produced into spherical raw material balls; (4) the spherical raw material balls are sintered under high temperature, and thereby the composite water purification material disclosed by the invention is prepared. The composite water purification material disclosed by the invention can effectively remove harmful elements and impurities in water, can release a variety of trace elements benefiting the human body, and can also effectively inhibit the breeding of bacteria.

The invention discloses an efficient breeding method for a spiral shell male tetraploid pure line. The method comprises the steps of tamoxifen feed preparation, tamoxifen feed putting for feeding female spiral shells, heat shock treatment, estrogen inhibitor solution preparation, estrogen inhibitor solution bathing, and obtaining of the spiral shell male tetraploid pure line. By means of the method, the 100% spiral shell male tetraploid pure line can be obtained. By means of the method, the spiral shell tetraploid can be effectively bred, the breeding rate and the fry rate of the tetraploid are increased, and a firm base is laid for producing triploid sterile fishes.

The invention provides a medical smell-removing antibacterial coating which is characterized by comprising the following components in percentage by weight: 20-30% of water, 40-55% of acrylic resin, 0-5.5% of water-based pigment, 0.7-2% of a coalescing agent, 0.4-1.25% of a dispersant, 0.15-0.5% of a leveling agent, 0.05-0.1% of a defoaming agent, 2-3.35% of a water-based adhesion accelerant, 5-7.65% of a negative ion additive, 0.22-1.38% of sodium hydrogen carbonate and 5-12.5% of nano silver. The medical smell-removing antibacterial coating is suitable for medical wall surfaces, has good peculiar smell removing ability and antibacterial property, can effectively kill bacteria and viruses and prevent the propagation of the bacteria and viruses, is good in adhesive powder, and is environmentally friendly and low in toxicity.

The invention provides a method for breeding insects by using hydroponic plants. The method comprises the following steps of (1) cultivation of host plants, wherein seeds of the host plants are planted onto a hydroponics tank cover plate after being soaked in warm water or a sterilizing agent solution; (2) inoculation of pests, wherein when the height of host plant seedlings is 4.5-5.5 cm, each seedling is inoculated with 5-50 aphids; (3) inoculation of natural enemy insects, wherein stem sections or leaves with the pests are placed on a hydroponics tank and inoculated with the natural enemy insects after 1-2 weeks. The method for breeding the insects by using the hydroponic plants has the advantages that coccinella septempunctata is bred by breeding the aphids using hydroponic broad beanseedlings, water which is treated and repeatedly utilized easily replaces sand which is treated and repeatedly utilized difficultly, and the planting method, a nutrient solution and the hydroponics tank are optimized, thereby providing the simple, efficient and low-cost method for breeding the coccinella septempunctata.

The invention provides a mildew-proof and crack-resistant light wall material, which is prepared from the following raw materials in parts by weight: 80-90 parts of foamed polystyrene, 35-45 parts ofglass fiber reinforced cement, 22-27 parts of diatom ooze, 17-22 parts of fly ash, 12-17 parts of yellow sand, 12-14 parts of caustic soda flakes, 7-12 parts of shale, 3-6 parts of carbon black, 2-4 parts of a water reducing agent, 3-5 parts of a surfactant, 2-4 parts of a binder, 1-2 parts of a reinforcing agent and 35-45 parts of water. The wall material is wide in raw material source, simple inpreparation method, low in cost, alkaline, not prone to breeding microorganisms, capable of effectively preventing mildew, excellent in mechanical property, extremely good in crack resistance and wide in application prospect.

The invention discloses a breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding. The method uses indica rice as female parent and japonica rice as male parent to hybridize and produce hybrid F1; if the anther of the hybrid F1 in the flowering stage does not dehisce to release pollens and pollen abortion is detected by a microscope after the pollen is dyed by iodine-potassium iodide solution, a new hybrid F1 generation is obtained by hybridizing the hybrid F1 as the female parent and the japonica rice; if the anther of the new hybrid F1 does not dehisce to release pollens and pollen abortion is detected by the microscope, the hybrid F1 backcrosses with the japonica rice as male parent; operating like these, the new sterile cytoplasmic source sterile line can be trans bred and the japonica rice restorer line is used for test-crossing; and if the fertility of test-crossing later generation is restored to normal fructification, the japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding is bred. The method is simple and convenient in operation, low in cost and short in breeding time, and can effectively breed the japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding.

The invention discloses a composition of a hydrophobic and antibacterial coating. The composition is prepared from, by weight, 100-150 parts of styrene-acrylic emulsion, 50-100 parts of acrylate emulsion, 30-50 parts of silicone acrylic emulsion, 1-10 parts of nanosilicon dioxide, 1-5 parts of inorganic antibacterial agent, 2-5 parts of thickening agent, 5-8 parts of dispersing agent, 1-3 parts ofwetting agent, 0.5-2 parts of defoaming agent and 80-110 parts of filler. The hydrophobic and antibacterial coating integrates multiple functions, can effectively prevent breeding of staphylococcus aureus, escherichia coli and other pernicious bacteria, prevent breeding of aspergillus niger and other fungi and improve the air quality in a humid environment; meanwhile, the waterproof performance is excellent, after the composition is utilized, the phenomenon that water leaks from a wall face can be effectively avoided, and meanwhile, the antibacterial effect is excellent.

The invention especially relates to a composition for preventing and treating the viral diseases of Gynura bicolor, belonging to the field of pesticides. The composition comprises the following raw materials by weight: 8 to 12 parts of white hyacinth beans, 12 to 18 parts of Cimicifuga foetida, 12 to 18 parts of Artemisia apiacea, 3 to 8 parts of mylabris, 12 to 18 parts of aloe, 2 to 6 parts of toad skin, 12 to 18 parts of Cortex Lycii, 3 to 8 parts of Flos Verbasci, 10 to 20 parts of trichosanthes roots, 12 to 18 parts of Breynia fruticosa, 8 to 12 parts of chasttree fruit, 8 to 12 parts ofBiebersteinia heterostemon, 10 to 20 parts of Polygonum perfoliatum, 8 to 12 parts of luffa stems, 2 to 8 parts of blue algae, 12 to 18 parts of compound bacteria and 10 to 20 parts of compound vitamin. The composition used for preventing and treating the viral diseases of Gynura bicolor and prepared from traditional Chinese medicines can effectively inhibit the propagation of viruses, controls the average incidence rate of the viral diseases of Gynura bicolor to be 2.26%, has a total effective rate of 94% or more in treatment of occurred viral diseases of Gynura bicolor, and is substantial inprevention and treatment effect.

The invention discloses a cleaning method for bacterial sludge breeding on the inner wall of the water inlet pipe of an NF / RO membrane unit, comprising the following steps: emptying the residual sewage in the water inlet pipe, cleaning the inner wall of the water inlet pipe with water, until the water quality of the eluate is The chromaticity is less than 10 times; the inner wall of the water inlet pipe and the NF / RO membrane unit are circularly cleaned with sodium bisulfite solution, so that the NF / RO membrane elements are soaked in the sodium bisulfite solution; the inner wall of the water inlet pipe is cleaned with cleaning agents Carry out soaking; wash the inner wall of the water inlet pipe with water; use neutralizing agents to circularly clean the inner wall of the water inlet pipe, and complete the cleaning of the bacterial sludge on the inner wall of the water inlet pipe of the NF / RO membrane unit. The cleaning method of the present invention can effectively remove the bacterial sludge grown on the inner wall of the water inlet pipe, has the advantages of simple process, easy operation, low treatment cost, high cleaning efficiency, good cleaning effect, etc., can meet the actual treatment needs, and has good advantages Application value and application prospect.

The invention belongs to the technical field of plant variety breeding, and particularly relates to a rapid breeding method of a bacterial wilt resistant fruit mulberry variety. The method comprises the following steps that branches of fruit mulberries are selected for seedling culture, after culture is conducted for 80-100 days, a ralstonia solanacearum bacterium solution is adopted for water culture, water culture is conducted for 30-45 days, finally survived fruit mulberries are the fruit mulberry variety with the bacterial wilt resistance, the fruit mulberry variety with the bacterial wiltresistance is adopted for planting culture in bacterial wilt serious disease land, the planting period is 2-3 years, the fruit mulberry variety with the bacterial wilt resistance can be bred, and theralstonia solanacearum bacterium solution is prepared from a bacterial strain G12-9 or a bacterial strain G12-50. Compared with a traditional method for culturing the bacterial wilt resistant fruit mulberry variety, the method has the advantages that the waste quantity of fruit mulberry materials is greatly reduced, the workload of variety breeding is remarkably reduced, and the problems of a long breeding period, complicated operation, difficulty in identification and the like of the bacterial wilt resistant fruit mulberry variety in the prior art are solved.

The invention discloses a method for high-efficiency breeding of male tetraploid pure line of tortoise, which comprises the following steps: preparation of tamoxifen feeding material, feeding of tamoxifen feeding material, heat shock treatment, preparation of estrogen inhibitor solution Preparation, uniform spraying with estrogen inhibitor solution, etc. Through this method, 100% male tetraploid pure line of tortoise can be obtained, which not only effectively breeds tetraploid of tortoise, but also improves the breeding rate and emergence rate of tetraploid; it lays the foundation for the production of triploid sterile tortoise solid foundation.

Disclosed is an artificial intelligent breeding method for vegetable comprising the steps of, (1) selecting n male and female parents to produce the first generation of the hybrid, surveying their main economy indexes, (2) inputting the survey data into computer, processing the data through data base management system, creating the mathematics model reflecting the relation between the first generation parent sample and the hybridization, (3) inputting the performance of the male parent and female parent into computer, forecasting the character of the first generation of hybrid through the mathematics model, (4) selecting several portions of the forecasted fine hybrid first generation, carrying out field sowing, surveying yield, quality, disease resistance and choosing novel crop breeds.

The invention provides an SNP marker associated with bacterial septicemia of schizothorax prenanti and application of the SNP marker. A nucleotide sequence of the SNP marker is shown in SEQ ID NO. 1; a 779th base of the sequence from 5' terminus is C or T. At an SNP site of the invention, the probability that the schizothorax prenanti having genotype CT is infected with bacterial septicemia is significantly higher than that of homozygous CC type individuals. Therefore, whether the schizothorax prenanti is easily infected with bacterial septicemia can be determined effectively by detecting the SNP of the schizothorax prenanti. The CT genotype individuals are knocked out in breeding of parents; and the marker of the invention is used for auxiliary breeding to accelerate a cultivation process of excellent disease-resistant varieties of the schizothorax prenanti.

The invention belongs to the technical field of agriculture, and discloses an application of a broom corn millet gene molecular marker in identification of broom corn millet yield-related traits. Theapplication comprises the following steps: designing PCR primers, and performing detection with a PCR method; carrying out parent selection and hybridization to obtain F1-generation seeds; planting the F1-generation seeds into seedlings, harvesting F2-generation seeds, sowing the F2-generation seeds, and harvesting hybrid filial generation; extracting total DNA and total RNA in leaf tissue of thehybrid filial generation at different growth and development stages for molecular identification; selfing the obtained filial generation containing the molecular marker for multiple generations to obtain an optimal filial generation; and carrying out asexual propagation on the obtained optimal hybrid filial generation in a tissue culture manner, culturing and screening tissue culture seedlings, and carrying out trait identification. According to the invention, the excellent yield trait variety of the broom corn millet can be effectively bred; and a new method for the molecular marker-assistedselective breeding of the broom corn millet is provided, and important significance in culturing high-yield broom corn millet varieties or researching is achieved.

The invention discloses an efficient breeding method for male tetraploid pure lines of soft-shelled turtles. The efficient breeding method comprises the following steps: preparing tamoxifen feeding materials, feeding female soft-shelled turtles with the tamoxifen feeding materials, carrying out heat shock treatment, preparing an estrogen inhibitor solution and obtaining the male tetraploid pure lines of the soft-shelled turtles. With the adoption of the method, the 100% male tetraploid pure lines of the soft-shelled turtles can be obtained; tetraploids of the soft-shelled turtles are effectively bred and the breeding rate and the yield of the tetraploids are improved; and a firm foundation is laid for the production of triploid sterile soft-shelled turtles.