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Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding

A hybrid japonica rice, cytoplasmic technology is applied in the field of breeding of new japonica cytoplasmic sterile lines, which can solve the problems of difficulty in new japonica cytoplasmic sterile lines, single cytoplasmic source, and the like, and achieve effective breeding. , the effect of short breeding time and easy to master

Inactive Publication Date: 2012-07-25
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem solved by the present invention is to overcome the defect of difficulty in selecting and breeding new japonica-type sterile cytoplasmic sterile lines that can be used for hybrid japonica rice breeding in the prior art, and the purpose of the invention is to break through the bottleneck of single japonica-type sterile cytoplasmic source and provide Breeding method of a new japonica-type sterile cytoplasmic sterile line

Method used

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  • Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding
  • Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding
  • Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding

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Embodiment 1

[0023] One, the present invention method " (indica rice / japonica rice) F 1 / japonica rice" hybridization and backcrossing

[0024] (1) In the summer of 2001 in Yunnan Province, China, the Korean indica rice variety Miryang 77 was crossed with the Yunnan japonica rice variety Heline 15, and Miryang 77 was used as the female parent and Heline 15 was used as the male parent to produce the hybrid F 1 (Miryang 77 / Hexi 15); the hybrid F was planted in winter in Hainan Province, China 1 Seeds were observed during the flowering period, and the anthers did not dehisce and loose powder appeared, and the hybrid F 1 The pollen was stained with iodine-potassium iodide solution to detect the pollen fertility under the microscope. The result of the microscope was that the pollen was aborted (that is, the rate of aborted pollen was 100%). The hybrid F 1 The plant was used as the female parent and crossed with Yunnan japonica variety Dianyu to produce a new hybrid F 1 generation.

[0025] ...

Embodiment 2

[0042] One, the present invention method " (indica rice / japonica rice) F 1 / japonica rice" hybridization and backcrossing

[0043] In addition to crossing the indica rice variety Minghui 63 from Fujian with the japonica variety Heline 34 from Yunnan, Minghui 63 was used as the female parent and Heline 34 was used as the male parent to produce a hybrid F 1 (Minghui 63 / Hexi 34); Hybrid F 1 Hybridization with Yunnan japonica rice variety Yumi 15 to produce a new hybrid 1 generation; with the new hybrid F 1 The generation plant was used as the female parent and Yumi 15 was used as the male parent for backcrossing to produce the first generation of backcrossed BC 1 and each backcross variety of the subsequent backcross generation is Yumi 15 and embodiment 1. One, the inventive method "(indica rice / japonica rice) F 1 / japonica rice" except that the hybridization and backcrossing methods are different, the remaining operations of steps (1), (2), (3) and (4) are all the same as th...

Embodiment 3

[0051] One, the present invention method " (indica rice / japonica rice) F 1 / japonica rice" hybridization and backcrossing

[0052] In addition to crossing the indica rice variety IR58025 of the Philippine International Rice Institute with the japonica rice variety Xiangzi in Yunnan in the summer of 2004, IR58025 was used as the female parent and Xiangzi was used as the male parent to produce a hybrid F 1 (IR58025 / fragrant purple); Hybrid F 1 (IR58025 / Xiangzi) was crossed with Yunnan japonica rice variety Helin 41 to produce a new hybrid F 1 generation, to the new hybrid F 1 The generation plant is the female parent and the combined line 41 is the male parent for backcrossing to produce the first generation of backcrossing BC 1 and the male parent of each backcross variety in the subsequent backcross generation is the combined line 41 and embodiment 1. One, the inventive method "(indica rice / japonica rice) F 1 / japonica rice" except that the hybridization and backcrossing m...

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Abstract

The invention discloses a breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding. The method uses indica rice as female parent and japonica rice as male parent to hybridize and produce hybrid F1; if the anther of the hybrid F1 in the flowering stage does not dehisce to release pollens and pollen abortion is detected by a microscope after the pollen is dyed by iodine-potassium iodide solution, a new hybrid F1 generation is obtained by hybridizing the hybrid F1 as the female parent and the japonica rice; if the anther of the new hybrid F1 does not dehisce to release pollens and pollen abortion is detected by the microscope, the hybrid F1 backcrosses with the japonica rice as male parent; operating like these, the new sterile cytoplasmic source sterile line can be trans bred and the japonica rice restorer line is used for test-crossing; and if the fertility of test-crossing later generation is restored to normal fructification, the japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding is bred. The method is simple and convenient in operation, low in cost and short in breeding time, and can effectively breed the japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding.

Description

technical field [0001] The invention relates to a method for selecting a japonica-type new cytoplasmic sterile line that can be used for hybrid japonica rice breeding, and belongs to the technical field of crop breeding. Background technique [0002] Cytoplasmic male sterility is an important basis for the utilization of heterosis in rice. Since the end of the 1960s, indica and japonica cytoplasmic male sterile lines have been successively bred in my country, and since the successful realization of three-line matching, hybrid rice has been popularized and applied in a large area. However, the source of rice sterile cytoplasm used in production (hereinafter referred to as: rice sterile cytoplasm source) has long-term singleness. In hybrid indica rice, more than 60% of the hybrid indica combinations popularized and used in the 1990s contained wild type Cytoplasm: For japonica hybrid rice, the sterile lines used in the cultivation and production of japonica hybrid rice combina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 文建成谭学林黄大军洪汝科金寿林谭亚玲李伟华顾晓明
Owner YUNNAN AGRICULTURAL UNIVERSITY
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