Efficient breeding method for male tetraploid pure lines of soft-shelled turtles

A tetraploid and male technology, which is applied in animal husbandry and other fields, can solve the problems of high-efficiency breeding of tetraploid all-male soft-shelled turtles that have not been seen, and achieve the effects of increasing the breeding rate and emergence rate, increasing the survival rate, and strengthening the formation probability

Inactive Publication Date: 2016-09-21
HUNAN UNIV OF ARTS & SCI
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the genetic breeding of soft-shelled turtles, so far there has been no report on the efficient breeding of tetraploid all-male soft-shelled turtles.

Method used

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  • Efficient breeding method for male tetraploid pure lines of soft-shelled turtles

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Experimental program
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Effect test

Embodiment 1

[0014] The efficient breeding method of male tetraploid pure line of soft-shelled turtle comprises the following steps:

[0015] (1) Provision of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95%, add it to the conventional feeding feed of soft-shelled turtles, and make a feeding material, in which the content of tamoxifen is 10g / 500g feed ,stand-by;

[0016] (2) Feeding: Before the female turtle lays eggs, feed tamoxifen feeding material to the female parent according to the conventional feed feeding method, three times a day, 15g feeding material per 100g turtle at 8:00 in the morning, and 15g feeding material per 100g turtle at noon. At 12:00, every 100g of soft-shelled turtle was fed with 15g of feeding material, at 6:00 pm, every 100g of soft-shelled turtle was fed with 10g of feeding material until laying eggs;

[0017] (3) Heat shock treatment: Put the fertilized eggs in a constant temperature incubator with a temperature of 32°C and a hu...

Embodiment 2

[0021] The efficient breeding method of male tetraploid pure line of soft-shelled turtle comprises the following steps:

[0022] (1) Preparation of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95% and add it to the conventional feeding feed for soft-shelled turtles to make a feeding material, in which the tamoxifen content is 8g / 500g feed ,stand-by;

[0023] (2) Feeding: Before the female turtle lays eggs, feed the female parent with tamoxifen according to the conventional feeding method, three times a day, 18g per 100g turtle at 8:00 in the morning, and 12:00 at noon. 00 Feed 15g of feeding material per 100g of soft-shelled turtle, and 10g of per 100g of soft-shelled turtle at 6:00 pm;

[0024] (3) Heat shock treatment: Put the fertilized eggs in a constant temperature incubator with a temperature of 28°C and a humidity of 60%. 3 minutes before a cleavage, quickly raise the temperature to 42°C and keep it for 5 minutes; then the temperature dr...

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Abstract

The invention discloses an efficient breeding method for male tetraploid pure lines of soft-shelled turtles. The efficient breeding method comprises the following steps: preparing tamoxifen feeding materials, feeding female soft-shelled turtles with the tamoxifen feeding materials, carrying out heat shock treatment, preparing an estrogen inhibitor solution and obtaining the male tetraploid pure lines of the soft-shelled turtles. With the adoption of the method, the 100% male tetraploid pure lines of the soft-shelled turtles can be obtained; tetraploids of the soft-shelled turtles are effectively bred and the breeding rate and the yield of the tetraploids are improved; and a firm foundation is laid for the production of triploid sterile soft-shelled turtles.

Description

technical field [0001] The invention relates to a polyploid breeding method of soft-shelled turtles, in particular to a method for efficiently breeding male tetraploid pure lines of soft-shelled turtles. Background technique [0002] Soft-shelled turtles, with Chinese soft-shelled turtles as the best, are also called turtles and soft-shelled turtles. Belonging to Reptiles, Turtles, and Turtles, diploids are common. Turtle meat is delicious and nutritious. It has the functions of clearing away heat and nourishing yin, calming the liver and quenching wind, softening and resolving hard masses, etc. Diploids grow slowly, and the breeding cycle Up to 4-5 years. Due to the sterility characteristics of triploids, soft-shelled turtles show more significant advantages in terms of growth speed, disease resistance, and meat quality. In the prior art, the fastest and most effective way to obtain triploid sterile soft-shelled turtles is to obtain tetraploid soft-shelled soft-shelled so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/0275A01K2207/25A01K2227/50
Inventor 杨品红韩庆邹万生
Owner HUNAN UNIV OF ARTS & SCI
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