Efficient breeding method for male tetraploid pure lines of soft-shelled turtles

Abstract

The invention discloses an efficient breeding method for male tetraploid pure lines of soft-shelled turtles. The efficient breeding method comprises the following steps: preparing tamoxifen feeding materials, feeding female soft-shelled turtles with the tamoxifen feeding materials, carrying out heat shock treatment, preparing an estrogen inhibitor solution and obtaining the male tetraploid pure lines of the soft-shelled turtles. With the adoption of the method, the 100% male tetraploid pure lines of the soft-shelled turtles can be obtained; tetraploids of the soft-shelled turtles are effectively bred and the breeding rate and the yield of the tetraploids are improved; and a firm foundation is laid for the production of triploid sterile soft-shelled turtles.

Description

technical field

[0001] The invention relates to a polyploid breeding method of soft-shelled turtles, in particular to a method for efficiently breeding male tetraploid pure lines of soft-shelled turtles. Background technique

[0002] Soft-shelled turtles, with Chinese soft-shelled turtles as the best, are also called turtles and soft-shelled turtles. Belonging to Reptiles, Turtles, and Turtles, diploids are common. Turtle meat is delicious and nutritious. It has the functions of clearing away heat and nourishing yin, calming the liver and quenching wind, softening and resolving hard masses, etc. Diploids grow slowly, and the breeding cycle Up to 4-5 years. Due to the sterility characteristics of triploids, soft-shelled turtles show more significant advantages in terms of growth speed, disease resistance, and meat quality. In the prior art, the fastest and most effective way to obtain triploid sterile soft-shelled turtles is to obtain tetraploid soft-shelled soft-shelled so...

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