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Colloidal gold detection test strip for dairy cow milk progesterone and preparation method thereof

A technology for detecting test strips and colloidal gold, applied in the field of biochemistry, can solve the problems of uncertainty in rectal examination and high price of B-ultrasound instruments

Inactive Publication Date: 2016-08-17
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of milk progesterone colloidal gold detection test strips of dairy cows, aiming to solve the problem of generally relying on rectal examination and B-ultrasound examination for dairy cow pregnancy detection in actual production, rectal examination has uncertainty and B The Problem with the High Price of Ultra Instruments

Method used

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  • Colloidal gold detection test strip for dairy cow milk progesterone and preparation method thereof
  • Colloidal gold detection test strip for dairy cow milk progesterone and preparation method thereof
  • Colloidal gold detection test strip for dairy cow milk progesterone and preparation method thereof

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preparation example Construction

[0071] The present invention is achieved in this way, the preparation method of a kind of dairy cow milk progesterone colloidal gold detection test paper strip comprises:

[0072] S101: Preparation of main solutions, including preparation of trisodium citrate solution, preparation of chloroauric acid solution, preparation of K 2 CO 3 Solution, preparation of PBS solution, preparation of BSA solution, preparation of sample pad treatment solution, preparation of gold standard complex solution;

[0073] S102: Preparation and identification of colloidal gold, including preparation of colloidal gold, quality identification of colloidal gold, preparation and purification of gold-labeled progesterone antibody complex, selection of antigen coating solution and gold-labeled diluent, and selection of gold-labeled antibody diluent and dilution selection, NC membrane selection and secondary antibody coating concentration selection, NC membrane blocking solution and blocking time selectio...

Embodiment 1

[0131] Determination of various properties of progesterone colloidal gold test strips

[0132] Milk sample test: Different batches of test strips were used to test the milk samples of 25 pregnant and 25 non-pregnant dairy cows confirmed by rectal method.

[0133] Determination of the detection limit of test strips: Progesterone standard solutions of different concentrations (concentrations of 1ng / mL, 3ng / mL, 5ng / mL, 7ng / mL, 9ng / mL, 11ng / mL, 13ng / mL, 15ng / mL , 17ng / mL), dissolved in methanol. After the test strips were assembled, 50 μL of progesterone standard solutions of different concentrations were added dropwise to the test strips, and after standing at room temperature for 5 minutes, PBS was used as a negative control to observe the test results.

[0134] Specificity test: 11-α-hydroxyprogesterone, estradiol, testosterone, follicle-stimulating hormone, estrogen, androstenedione, cortisol, and corticosterone were prepared in PBS to a standard concentration solution of 100...

Embodiment 2

[0176] Embodiment two, the clinical application of test two colloidal gold test strips

[0177] In this embodiment, the standard intensive cattle farm in the eastern part of Heilongjiang is selected for the clinical application of progesterone colloidal gold test strips. The test strips are mainly used to detect the pregnancy of dairy cows, and the performance of the test strips is improved through the test results. The relationship between progesterone content in the body and certain reproductive disorders.

[0178] Materials and Methods

[0179] 1.1 Main Instruments

[0180] The centrifuge Anke TDL80-2B was purchased from Shanghai Anting Scientific Instrument Company; the ultra-low temperature refrigerator; the T6 UV-Vis spectrophotometer was purchased from Beijing General Instrument Co., Ltd.; the incubator was purchased from Shanghai Yuejin Medical Devices No. 1 Factory.

[0181] 1.2 Main detection reagents

[0182] Progesterone ELISA kit was purchased from Kamishu Shan...

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Abstract

The invention discloses a dairy cow milk progesterone colloidal gold test strip and a preparation method thereof. The preparation method of the dairy cow milk progesterone colloidal gold test strip comprises the steps of preparation of main solutions, namely preparing a trisodium citrate solution, preparing a chloroauric acid solution, preparing a K2CO3 solution, preparing a PBS, a BSA (Bovine Serum Albumin) solution, preparing a sample pad treating fluid and preparing a gold mark combination solution; and preparation and authentication of colloidal gold, namely preparing colloidal gold, authenticating the quality of colloidal gold, preparing and purifying a gold marked progesterone antibody compound, selecting an antigen coating solution and a gold mark diluting solution, selecting gold marked antibody diluent and dilution, selecting an NC film and secondary antibody coating concentration, selecting an NC film blocking liquid and blocking time, selecting the sample pad, and assembling the colloid gold test strip. The test strip disclosed by the invention has a good stable effect, is good in stability and high in flexibility and sensitivity, is in favor of clinical application and the guidance of clinical hybridization work, and is capable of effectively guiding a worker to diagnose and treat diseases, and the pregnancy rate is improved.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a milk cow milk progesterone colloidal gold detection test strip and a preparation method thereof. Background technique [0002] In actual production, the pregnancy detection of dairy cows generally relies on rectal examination and B-ultrasound examination. The uncertainty of rectal examination and the high price of B-ultrasound equipment have become the disadvantages of clinical application. Progesterone is a steroidal hormone. As an indicator of the ovary, monitoring the changes in the content of progesterone secreted in dairy cows can effectively detect pregnancy and reproductive status. It is an effective method for early pregnancy inspection and reproductive disease monitoring. [0003] The colloidal gold test strip detection method has the advantages of easy learning, rapidity, convenience, low cost, no need for any equipment and non-toxic reagents, and is suitable f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/532
CPCG01N33/532G01N33/543G01N33/74
Inventor 夏成吴凌徐闯郑家三包凯孙雨航李颖许楚楚张洪友
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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