Rapid breeding method of bacterial wilt resistant fruit mulberry variety

A technology for resistance to bacterial wilt and bacterial wilt is applied in the field of rapid breeding of fruit and mulberry varieties with resistance to bacterial wilt, which can solve problems such as infection and economic loss, and achieve the advantages of excellent variety quality, reduced waste and reduced workload. Effect

Inactive Publication Date: 2021-01-19
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The host range of R. solanacearum is very wide. It has been found that hundreds of plants in 44 families can be infected by it, and new hosts are constantly being discovered, which brings great economic losses to crop production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A rapid breeding method for fruit mulberry varieties resistant to bacterial wilt:

[0027] 1) Using the fruit and mulberry resources in Guangdong as breeding materials, the selected variety resources (all exist in the South China sub-nursery of national germplasm resources) include the following, fruit selection 04-76, fruit selection 04-78, fruit selection 04-25, Yuguo 06-15, Guoxuan 04-7, Guoxuan 04-74, Yuguo 06-7, Guoxuan 04-111, Guoxuan 04-34, Guoxuan 04-43, Yuguo 04-35, Guoxuan 04-52, Guoxuan 04-75, Guoxuan 04-62, Yuguo 06-5, Guoxuan 04-73, Guoxuan 04-97, Guoxuan 04-46, Guoxuan 04-93, Guoxuan 04- 52. Fruit selection 04-72, Fruit selection 04-132, Fruit selection 04-139, Fruit selection 04-136, Fruit selection 04-137, Fruit selection 04-117, Fruit selection 04-75, Fruit selection 04-125, Guoxuan 04-129, Guoxuan 04-120, Guoxuan 04-123, Guoxuan 04-122, Guoxuan 06-11, Yuguo 06-10, Yuguo 06-16, Yuguo 06-8, Guoxuan 04-12, Yuguo 06-11, Guoxuan 04-14, Yuguo 06-13, Guoxua...

Embodiment 2

[0034] A rapid breeding method for fruit mulberry varieties resistant to bacterial wilt:

[0035] 1) same as embodiment 1 step 1);

[0036] 2) Cultivate the seedlings from the branches of the above-mentioned fruit mulberry varieties by cutting method, cut the roots of the seedlings obliquely after 90 days of cultivation, and directly soak them in the bacterial solution of R. solanacearum for hydroponics. The concentration used in the early hydroponics is 0.4× 10 9 The cfu / mL G12-9 R. solanacearum bacterial solution was hydrocultured for 10 days at a temperature of 30-32°C and an ambient humidity of 70-75%;

[0037] The medium-term hydroponics adopts a concentration of 0.9×10 9 The cfu / mL G12-9 Ralstonia solanacearum solution was hydrocultured for 10 days at a temperature of 32-34°C and an ambient humidity of 76-80%;

[0038] In the later stage of hydroponics, the concentration was 1.0×10 9 The cfu / mL G12-9 R. solanacearum bacteria liquid was cultured in water for 10 days, ...

Embodiment 3

[0042] A rapid breeding method for fruit mulberry varieties resistant to bacterial wilt:

[0043]1) same as embodiment 1 step 1);

[0044] 2) Cultivate the seedlings from the branches of the above-mentioned fruit mulberry varieties by cutting method, and cut the roots of the seedlings obliquely after 100 days of cultivation, and directly soak them in the bacterial solution of R. solanacearum for hydroponics. 10 9 The cfu / mL G12-50 Ralstonia solanacearum solution was hydrocultured for 13 days at a temperature of 30-32°C and an ambient humidity of 70-75%;

[0045] The mid-term hydroponics use a concentration of 1.0×10 9 The cfu / mL G12-50 Ralstonia solanacearum solution was hydrocultured for 14 days at a temperature of 31-33°C and an ambient humidity of 74-79%;

[0046] In the later stage of hydroponics, the concentration used was 0.9×10 9 The cfu / mL G12-50 Ralstonia solanacearum solution was hydrocultured for 13 days at a temperature of 32-35°C and an ambient humidity of 78-...

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Abstract

The invention belongs to the technical field of plant variety breeding, and particularly relates to a rapid breeding method of a bacterial wilt resistant fruit mulberry variety. The method comprises the following steps that branches of fruit mulberries are selected for seedling culture, after culture is conducted for 80-100 days, a ralstonia solanacearum bacterium solution is adopted for water culture, water culture is conducted for 30-45 days, finally survived fruit mulberries are the fruit mulberry variety with the bacterial wilt resistance, the fruit mulberry variety with the bacterial wiltresistance is adopted for planting culture in bacterial wilt serious disease land, the planting period is 2-3 years, the fruit mulberry variety with the bacterial wilt resistance can be bred, and theralstonia solanacearum bacterium solution is prepared from a bacterial strain G12-9 or a bacterial strain G12-50. Compared with a traditional method for culturing the bacterial wilt resistant fruit mulberry variety, the method has the advantages that the waste quantity of fruit mulberry materials is greatly reduced, the workload of variety breeding is remarkably reduced, and the problems of a long breeding period, complicated operation, difficulty in identification and the like of the bacterial wilt resistant fruit mulberry variety in the prior art are solved.

Description

technical field [0001] The invention belongs to the technical field of plant variety breeding, and in particular relates to a rapid breeding method for bacterial wilt-resistant fruit mulberry varieties. Background technique [0002] Bacterial wilt is a devastating disease caused by Ralstonia solanacearum and widely distributed in tropical, subtropical and some warm regions of the world. The host range of R. solanacearum is very wide. It has been found that hundreds of plants in 44 families can be infected by it, and new hosts are constantly being discovered, which brings great economic losses to crop production. [0003] At present, one of the effective methods to prevent the spread of R. solanacearum is to use sterile plant propagation materials. There are also rapid detection of R. solanacearum in plants, soil and water samples for timely detection and remove germs to prevent their spread. [0004] Mulberry bacterial wilt is a vascular disease in which bacterial wilt inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G2/10A01G7/06A01G17/00
CPCA01G31/00A01G2/10A01G17/005A01G7/06
Inventor 唐翠明罗国庆戴凡炜王振江钟建武林森李智毅高倩云王圆
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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