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Efficient breeding method for male tetraploid pure line of tortoises

A tetraploid and tortoise technology, which is applied in animal husbandry and other fields, can solve problems such as the high-efficiency breeding of male tetraploids that have not been seen in tortoises, and achieve the effects of increasing the breeding rate and emergence rate, inhibiting DNA splitting, and reducing adverse effects

Inactive Publication Date: 2016-10-26
HUNAN UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the genetic breeding of tortoises, so far there has been no report on the efficient breeding of male tetraploids in tortoises.

Method used

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  • Efficient breeding method for male tetraploid pure line of tortoises

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The method for the efficient breeding of tortoise male tetraploid pure line includes the following steps:

[0015] (1) Preparation of tamoxifen feeding material: take tamoxifen powder with a purity of more than 95% and add it to tortoise regular feeding feed to make feeding material, in which tamoxifen content is 16g / 500g feeding material ,stand-by;

[0016] (2) Feeding: 5 days before the mother tortoise lays eggs, the mother will be fed with tamoxifen feed according to the conventional feeding method, twice a day, at 8:00 in the morning every 100g turtle will be fed with 30g feed At 6:00 pm, every 100g tortoise will be fed 15g of feed until the egg is laid;

[0017] (3) Heat shock treatment: Put the fertilized egg of step (2) in a constant temperature incubator with a temperature of 27°C and a humidity of 80%. 3 minutes before a cleavage, the temperature is quickly raised to 39°C and kept for 5 minutes; The temperature drops to 29°C;

[0018] (4) Preparation of estrogen inhi...

Embodiment 2

[0021] The method for the efficient breeding of tortoise male tetraploid pure line includes the following steps:

[0022] (1) Preparation of tamoxifen feeding material: take tamoxifen powder with a purity of more than 95% and add it to tortoise regular feeding feed to make feeding material, in which tamoxifen content is 16g / 500g feeding material ,stand-by;

[0023] (2) Feeding: 10 days before the mother tortoise lays eggs, the mother will be fed with tamoxifen feed according to the conventional feed method, twice a day, at 8:00 in the morning, 25g per 100g tortoise, in the afternoon Feed 15g per 100g tortoise at 6:00 until laying eggs;

[0024] (3) Heat shock treatment: Put the fertilized egg of step (2) in a constant temperature incubator with a temperature of 34°C and a humidity of 70%. 2 minutes before a cleavage, the temperature is quickly raised to 40°C and kept for 3 minutes, then The temperature drops to 33°C;

[0025] (4) Preparation of estrogen inhibitor solution: Dissolve ...

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Abstract

The invention discloses a method for high-efficiency breeding of male tetraploid pure line of tortoise, which comprises the following steps: preparation of tamoxifen feeding material, feeding of tamoxifen feeding material, heat shock treatment, preparation of estrogen inhibitor solution Preparation, uniform spraying with estrogen inhibitor solution, etc. Through this method, 100% male tetraploid pure line of tortoise can be obtained, which not only effectively breeds tetraploid of tortoise, but also improves the breeding rate and emergence rate of tetraploid; it lays the foundation for the production of triploid sterile tortoise solid foundation.

Description

Technical field [0001] The invention relates to a tortoise polyploid breeding method, in particular to a method for efficiently breeding a tortoise male tetraploid pure line. Background technique [0002] The tortoise belongs to the class of Reptiles, Turtles and Turtles. It is an existing ancient reptile. The most common is diploid. It has a very strong carapace. The growth rate of female tortoise is about 15 grams. 50 grams of age turtles, 100 grams of third age turtles, 200 grams of fourth age turtles, 250 grams of ageless turtles, male tortoises grow slowly, and the largest sexually mature individual is generally less than 250 grams. Due to the sterile characteristics of triploids, tortoises show more significant advantages in growth speed, disease resistance, and meat quality. In the prior art, to obtain triploid sterile tortoises, the quickest and most effective way is to obtain the tetraploid tortoise and diploid tortoise ploidy. Then it is necessary to establish a geneti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/0275
Inventor 杨品红韩庆黄春红
Owner HUNAN UNIV OF ARTS & SCI
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