Application of broom corn millet gene molecular marker in identification of broom corn millet yield-related traits

Abstract

The invention belongs to the technical field of agriculture, and discloses an application of a broom corn millet gene molecular marker in identification of broom corn millet yield-related traits. Theapplication comprises the following steps: designing PCR primers, and performing detection with a PCR method; carrying out parent selection and hybridization to obtain F1-generation seeds; planting the F1-generation seeds into seedlings, harvesting F2-generation seeds, sowing the F2-generation seeds, and harvesting hybrid filial generation; extracting total DNA and total RNA in leaf tissue of thehybrid filial generation at different growth and development stages for molecular identification; selfing the obtained filial generation containing the molecular marker for multiple generations to obtain an optimal filial generation; and carrying out asexual propagation on the obtained optimal hybrid filial generation in a tissue culture manner, culturing and screening tissue culture seedlings, and carrying out trait identification. According to the invention, the excellent yield trait variety of the broom corn millet can be effectively bred; and a new method for the molecular marker-assistedselective breeding of the broom corn millet is provided, and important significance in culturing high-yield broom corn millet varieties or researching is achieved.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to the application of a broomcorn gene molecular marker in identifying yield-related traits of broomcorn millet. Background technique [0002] At present: broomcorn millet (Panicum miliaceum L.) belongs to the genus Panicum of Gramineae, also known as broomcorn millet and millet. Rich, more than 8,000 copies have been collected and preserved so far. Millet has a short growth period, is resistant to drought and barrenness, and belongs to two types of japonica and glutinous rice. It is an important disaster-resistant crop; the planting area of ​​millet in China is about 1 million hm 2 , ranking second in the world, and it has a very special role in the development of the national economy. effect. Millet contains very rich nutrition, 8.6%-15.5% of millet grain content is protein, 2.6%-6.9% is fat, 67.6%-75.1% is starch, 3.5%-4.4% is dietary fiber, 1.3%-4.3% is ash. ...

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the problems and defects in the prior art are: the existing broomcorn breeding method is time-consuming and laborious, and there is no method for carrying out broomcorn broom breeding by molecular markers in the prior art

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