Efficient breeding method for silver-fish triploid

Abstract

The invention discloses a high-efficiency breeding method for whitebait triploids, comprising the following steps: preparation of tamoxifen inhibitors, preparation of tamoxifen injection, injection treatment of female parents, cold shock + hydrostatic pressure treatment, female Preparation of hormone inhibitor solution, taking fertilized eggs and putting them in the estrogen inhibitor solution, obtaining the pure line of whitebait male tetraploid, taking the male tetraploid as the male parent, taking the common diploid as the female parent, and adopting backcross way to obtain 100% triploid. By adopting the method of the present invention, 100% whitebait triploid pure lines can be obtained, which improves the breeding rate and emergence rate of triploids, reduces the trouble of breeding and selection, and is more important for the germplasm and germplasm of natural aquatic products. The ecological environment is pollution-free.

Description

technical field [0001] The invention relates to a polyploid breeding method for whitebait, in particular to a method for efficiently breeding triploid whitebait. Background technique [0002] Whitebait is slightly round in body length, tender and transparent, silver in color, short in life cycle, strong in fecundity and settlement ability, and high in nutritional value. It is one of the important economic fish in my country. The sterility characteristics of triploid whitebait make it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, the sterile triploid whitebait is obtained by hybridization between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, it is necessary to establish genetically stable and extremely high-purity tetraploid fish line. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibi...

AI Technical Summary

Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle

In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

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