Efficient breeding method for silver-fish triploid

A technology of triploid and whitebait, which is applied in animal husbandry and other fields, can solve the problems of difficulty in obtaining a quadruple system of purity, difficulty in obtaining a quadruple system, and low success rate, so as to improve the breeding rate, emergence rate, and survival rate , the effect of reducing trouble

Inactive Publication Date: 2016-10-26
HUNAN UNIV OF ARTS & SCI
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Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle
In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

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  • Efficient breeding method for silver-fish triploid

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Effect test

Embodiment 1

[0017] The high-efficiency breeding method of triploid silverfish comprises the steps:

[0018] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the content of tamoxifen is about 80mg / ml. use;

[0019] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 200ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is about 0.4mg / ml, stand-by;

[0020] (3) Injection treatment of the female parent: before the artificial egg laying of the female parent, artificially inject tamoxifen injection into the female parent according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 150 μg / kg;

[0021] (4) Cold shock + hydrostatic pressure treatment: During...

Embodiment 2

[0026] The high-efficiency breeding method of triploid silverfish comprises the steps:

[0027] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 50mg / ml, and wait for use;

[0028] (2) Preparation of Tamoxifen Injection: Take the above-mentioned mother liquor and directly add it to the prepared oxytocic solution at a ratio of 1ml of tamoxifen mother liquor to 500ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is 0.1mg / ml, stand-by;

[0029] (3) Injection treatment of the female parent: before the artificial egg laying of the female parent, artificially inject tamoxifen injection into the female parent according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 200 μg / kg;

[0030] (4) Cold shock + hydrostatic pressure treatment: Du...

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Abstract

The invention discloses a high-efficiency breeding method for whitebait triploids, comprising the following steps: preparation of tamoxifen inhibitors, preparation of tamoxifen injection, injection treatment of female parents, cold shock + hydrostatic pressure treatment, female Preparation of hormone inhibitor solution, taking fertilized eggs and putting them in the estrogen inhibitor solution, obtaining the pure line of whitebait male tetraploid, taking the male tetraploid as the male parent, taking the common diploid as the female parent, and adopting backcross way to obtain 100% triploid. By adopting the method of the present invention, 100% whitebait triploid pure lines can be obtained, which improves the breeding rate and emergence rate of triploids, reduces the trouble of breeding and selection, and is more important for the germplasm and germplasm of natural aquatic products. The ecological environment is pollution-free.

Description

technical field [0001] The invention relates to a polyploid breeding method for whitebait, in particular to a method for efficiently breeding triploid whitebait. Background technique [0002] Whitebait is slightly round in body length, tender and transparent, silver in color, short in life cycle, strong in fecundity and settlement ability, and high in nutritional value. It is one of the important economic fish in my country. The sterility characteristics of triploid whitebait make it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, the sterile triploid whitebait is obtained by hybridization between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, it is necessary to establish genetically stable and extremely high-purity tetraploid fish line. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/0275
Inventor 杨品红罗玉双石彭灵
Owner HUNAN UNIV OF ARTS & SCI
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