Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines

Abstract

The invention discloses a method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines, comprising: hybridizing a cytoplasmic male-sterile line DHCMS08-5-3-6-9 trans-bred with a Brassica oleracea DH line as a female parent and the Brassica oleracea DH line DH05X-1-5-9-8 line as a male parent to prepare DHCMS08-5-5-6-9*DH05X-1-5-9-8 hybrid first-generation combination, named as Qingan 1562. The method of the invention is a method to breed a mid-early maturity novel variety of Brassica oleracea by using two DH lines, a novel hybrid first-generation variety of Brassica oleracea is bred through DH liens with genotype purity of 100% created by culturing isolated microspores of Brassica oleracea, the novel hybrid first-generation variety of Brassica oleracea has highly uniform economic traits and completely exhibited target traits, and the breeding of disease-resistant high-quality high-yield variety breeding targets is achieved.

Description

technical field [0001] The invention belongs to the discipline of crop genetics and breeding in agricultural science and technology, and specifically relates to a method for breeding a new cabbage variety by using two DH lines. Background technique [0002] Breeding of new varieties of cabbage is one of the main research projects for the selection and breeding of vegetable crops established by the state for a long time. The heterosis of cabbage is obvious, and the new varieties cultivated are all first-generation hybrids; the selection of excellent inbred lines, cytoplasmic male sterile lines and the utilization of heterosis have become one of the effective ways to breed new varieties with multiple objectives. The collocation and planting of new varieties of cabbage plays a leading role in the adjustment of agricultural industrial structure and the increase of farmers' income. Cabbage varieties are listed in multiple seasons and crops, which play a key role in alleviating...

AI Technical Summary

Problems solved by technology

[0004] Aiming at many problems and defects such as easy degeneration of inbred lines, poor hybrid uniformity, and insufficient performance of heterosis in the conventional technology of using two inbred lines to prepare the first generation of hybrid varieties, the purpose of the present invention is to provide a method using two DH lines A method for breeding a new cabbage variety. The DH line of the cabbage variety bred by this method has a small number of self-generations per plant, strong growth potential, high seed yield in the first generation of hybrids, and the uniformity of the first generation of hybrids prepared by two highly homozygous DH lines High, its bred varieties have undergone comparative tests, variety regional tests and production demonstrations, showing significant heterosis, completely consistent traits among hybrid plants, excellent comprehensive economic traits, outstanding high-yield, strong variety adaptability, stable yield-increasing advantages, and passed the national appraisal Naming "Qingan 1562" cabbage variety

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