Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines

A new variety, the technology of cabbage, which is applied in the field of crop genetics and breeding, can solve the problems of strong growth potential, high reproductive seed yield, and insufficient expression of heterosis

Inactive Publication Date: 2017-02-22
NORTHWEST A & F UNIV
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  • Application Information

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Problems solved by technology

[0004] Aiming at many problems and defects such as easy degeneration of inbred lines, poor hybrid uniformity, and insufficient performance of heterosis in the conventional technology of using two inbred lines to prepare the first generation of hybrid varieties, the purpose of the present invention is to provide a method using two DH lines A method for breeding a new cabbage variety. The DH line of the cabbage variety bred by this method has a small number of self-generations per plant, strong growth potential, high seed y...

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  • Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines
  • Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines
  • Method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines

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Embodiment 1

[0028] 1. Breeding and Character Performance of Two DH Line Parents of Qingan 1562 First Hybrid Variety

[0029] 1) Breeding of female germplasm material DHCMS08-5-3-6-9

[0030] (1) Breeding of DH08-5-3-6-9

[0031] figure 1 The breeding map of the maintainer line of the female parent DHCMS08-5-3-6-9 cytoplasmic male sterile line was given. The female parent maintainer line DH08-5-3-6-9 was developed by the School of Horticulture of West Agriculture and Forestry University in 2007 Two selected cabbage inbred lines MP01-2566×Y03-3621 were prepared as F1, and the F1 microspores were cultured ( image 3 DH08-5-3-6-9DH strain) and an excellent DH line ( Figure 4 DH line of DH08-5-3-6-9), agronomic traits are highly uniform, disease resistance and high quality (Table 1), high combining ability.

[0032] (2) DHCMS08-5-3-6-9 transfer

[0033] figure 1 The breeding map of the female parent DHCMS08-5-3-6-9 cytoplasmic male sterile line was given. The DHCMS08-5-3-6-9 cytoplas...

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Abstract

The invention discloses a method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines, comprising: hybridizing a cytoplasmic male-sterile line DHCMS08-5-3-6-9 trans-bred with a Brassica oleracea DH line as a female parent and the Brassica oleracea DH line DH05X-1-5-9-8 line as a male parent to prepare DHCMS08-5-5-6-9*DH05X-1-5-9-8 hybrid first-generation combination, named as Qingan 1562. The method of the invention is a method to breed a mid-early maturity novel variety of Brassica oleracea by using two DH lines, a novel hybrid first-generation variety of Brassica oleracea is bred through DH liens with genotype purity of 100% created by culturing isolated microspores of Brassica oleracea, the novel hybrid first-generation variety of Brassica oleracea has highly uniform economic traits and completely exhibited target traits, and the breeding of disease-resistant high-quality high-yield variety breeding targets is achieved.

Description

technical field [0001] The invention belongs to the discipline of crop genetics and breeding in agricultural science and technology, and specifically relates to a method for breeding a new cabbage variety by using two DH lines. Background technique [0002] Breeding of new varieties of cabbage is one of the main research projects for the selection and breeding of vegetable crops established by the state for a long time. The heterosis of cabbage is obvious, and the new varieties cultivated are all first-generation hybrids; the selection of excellent inbred lines, cytoplasmic male sterile lines and the utilization of heterosis have become one of the effective ways to breed new varieties with multiple objectives. The collocation and planting of new varieties of cabbage plays a leading role in the adjustment of agricultural industrial structure and the increase of farmers' income. Cabbage varieties are listed in multiple seasons and crops, which play a key role in alleviating...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 张恩慧程永安杨安平许忠民刘争张孟利李思蓓
Owner NORTHWEST A & F UNIV
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