Method for producing chilli kernel male sterile plants in large scale

Abstract

The invention provides a method for producing chilli kernel male sterile plants in a large scale. The method comprises the following steps of: inducing an adventitious bud to sprout by a stem section with an axillary bud of a chilli kernel male sterile dual-purpose system sterile plant and forming a plantlet from the adventitious bud; inducing the adventitious bud to sprout by a stem section with an axillary bud of the plantlet and forming a secondary plantnet from the adventitious bud; forming the plantnet through repeatedly subculturing and inducing the axillary bud; forming a large quantity of plantnets through tissue culture; dividing the plantnets to induce and form adventitious roots; and finally, forming a large quantity of chilli kernel male sterile dual-purpose system sterile plants through planting regeneration plants in an open farmland.

Description

technical field [0001] The invention mainly relates to a method for large-scale production of sterile plants of capsicum genital male sterile dual-purpose line by using plant tissue culture technology, in particular to a method for increasing the number of regenerated plants. Background technique [0002] Capsicum is a very important solanaceous vegetable. It is not only widely used as seasoning, but also developed for health food and medicine. The hybrid advantage of pepper is very obvious. Using the male sterile line as the female parent to produce the first generation of hybrid seeds is the main means of utilizing the hybrid advantage of pepper. Using the male sterile line of pepper as the female parent for the production of hybrid peppers, the sowing There are 50% fertile plants in the capsicum male sterile dual-purpose line, and all the fertile plants need to be pulled out at the flowering stage. Sometimes, the purity of hybrid seeds is low because the fertile plants ar...

AI Technical Summary

Problems solved by technology

The hybrid advantage of pepper is very obvious. Using the male sterile line as the female parent to produce the first generation of hybrid seeds is the main means of utilizing the hybrid advantage of pepper. Using the male sterile line of pepper as the female parent for the production of hybrid peppers, the sowing There are 50% of the fertile plants in the male sterile dual-purpose line of pepper, and all the fertile plants need to be pulled out at the flowering stage, sometimes because the fertile plants are not completely pulled out, it is easy to cause low purity of hybrid seeds

[0003] So far, the sterile plants of the GMS lines of pepper used for seed production are mainly obtained by pulling out 50% of the fertile plants in the GMS lines at the flowering stage, and it takes a lot of money. Manpower; In addition, the sterile plants of the capsicum genital male sterile dual-purpose line used for seed production are also obtained through tissue culture of leaves, etc. to obtain regenerated plants, but it is very difficult to obtain regenerated plants through tissue culture of leaves, etc., and the variation is relatively large. Seriously, it is not practical; there is another way to obtain sterile plants of capsicum genital male sterile line for seed production is through cutting propagation, but the propagation coefficient of cutting propagation is small, multi-generation propagation and degeneration The phenomenon