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High-grade bletilla striata tissue culture method

A technology of bletilla striata and bletilla striata, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that the excellent characteristics of bletilla striata cannot be inherited, the traits of offspring are different, and plants cannot be obtained, so as to achieve good economic value and popularization Significance, small variation rate, consistent effect of traits

Inactive Publication Date: 2019-01-15
河南云帮农业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bletilla striata propagation currently adopts the main methods of aseptic sowing of Bletilla striata seeds in tissue culture room and direct seeding method of Bletilla striata seeds in greenhouse. Both of these methods use seeds for propagation, and the plants that are consistent with the parents cannot be obtained. The variation rate is high, and the offspring have different characters. The excellent characteristics of Bletilla striata are inherited
The bletilla striata division method can also guarantee the excellent characteristics of bletilla striata, but the propagation speed is slow and the number of obtained is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A good bletilla striata tissue culture method specifically comprises the following steps:

[0017] (1) Selection of fine bletilla striata: select a group of bletilla striata seedlings with strong plants, pure purple flowers, large bulbs, fast growth, wide leaves, dark green leaves without spots, and tall and compact bletilla striata seedlings from the same-age fine bletilla striata varieties planted;

[0018] (2) Disinfection treatment: adopt excellent Bletilla striata bulbs with buds on the ultra-clean workbench, cut out the young shoots of Bletilla striata with a little bulb, disinfect with 75% alcohol for 10 seconds, use sterile water to clean, and use 0.1% mercuric chloride Sterilize for 12 minutes, rinse with sterile water 4 times, then dry the water on the buds with sterilized filter paper, and plant the cut shoots of Bletilla striata in MS, BA content 1mg / L, NAA content 0.1mg / L, banana puree Bletilla striata bud medium with a content of 50g / L was sent to the cult...

Embodiment 2

[0025] A good bletilla striata tissue culture method specifically comprises the following steps:

[0026] (1) Selection of fine bletilla striata: select a group of bletilla striata seedlings with strong plants, pure purple flowers, large bulbs, fast growth, wide leaves, dark green leaves without spots, and tall and compact bletilla striata seedlings from the same-age fine bletilla striata varieties planted;

[0027] (2) Disinfection treatment: adopt excellent Bletilla striata bulbs with buds on the ultra-clean workbench, cut out the young shoots of Bletilla striata with a little bulb, disinfect with 75% alcohol for 10 seconds, use sterile water to clean, and use 0.1% mercuric chloride Sterilize for 15 minutes, rinse with sterile water 5 times, then dry the water on the buds with sterilized filter paper, and plant the cut young shoots of Bletilla striata in MS, BA content 1mg / L, NAA content 0.1mg / L, banana puree Bletilla striata bud medium with a content of 50g / L was sent to th...

Embodiment 3

[0034] A good bletilla striata tissue culture method specifically comprises the following steps:

[0035] (1) Selection of fine bletilla striata: select a group of bletilla striata seedlings with strong plants, pure purple flowers, large bulbs, fast growth, wide leaves, dark green leaves without spots, and tall and compact bletilla striata seedlings from the same-age fine bletilla striata varieties planted;

[0036] (2) Disinfection treatment: adopt excellent Bletilla striata bulbs with buds on the ultra-clean workbench, cut out the young shoots of Bletilla striata with a little bulb, disinfect with 75% alcohol for 10 seconds, use sterile water to clean, and use 0.1% mercuric chloride Sterilize for 13 minutes, rinse with sterile water for 4 times, then dry the water on the buds with sterilized filter paper, and plant the cut young shoots of Bletilla striata in MS, BA content 1mg / L, NAA content 0.1mg / L, banana puree Bletilla striata bud medium with a content of 50g / L was sent to ...

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PUM

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Abstract

The invention relates to a high-grade bletilla striata tissue culture method particularly including steps of: 1) selection of seeds of high-grade bletilla striata; 2) disinfection; 3) germination reproduction; 4) seedling strengthening culture; 5) rooting culture; 6) acclimatization and seedling hardening; and 7) transplanting. The method guarantees the descendants to be tidy and uniform, is low in mutation rate, can hold the advantageous features and consistent characters of the high-grade plants and can effectively increase yield of the bletilla striata, and has excellent economic value andpromotion value.

Description

technical field [0001] The invention relates to the technical field of bletilla striata planting, in particular to an excellent bletilla striata tissue culture method. Background technique [0002] Bletilla striata is a plant of the genus Bletilla striata in the family Orchidaceae, also known as small bletilla striata, lotus and grass, snow as the end, etc. It is a second-class endangered plant in the country. Bletilla striata is native to my country and widely distributed in the provinces of the Yangtze River Basin. It is also distributed in North Korea and Japan, in the damp places under the forest in wild valleys. It has been used as traditional Chinese medicine for thousands of years, and its main functions are to suppress sores and stop bleeding, nourish the lungs, reduce swelling and promote muscle growth, etc. Bletilla striata is a perennial plant in the family Orchidaceae. Its roots are used as medicine, which has the effects of stopping bleeding, reducing swelling,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄春晓赵莉王伟李战旗李好胜阙莎莎周毅冯慧娜杨丙亮
Owner 河南云帮农业科技有限公司
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