A kind of tissue culture rapid propagation method of double poinsettia

A technology of tissue culture rapid propagation and poinsettia, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of long healing time of incisions, long rooting time, perishable branches, etc., and achieves the convenience of transplanting and planting The effects of management, neat seedlings, and shortened growth cycle

Active Publication Date: 2020-05-08
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing double-petalled poinsettia is generally propagated by cuttings or layering. Cuttings mainly include semi-hard branch cuttings, tender branch cuttings, and old root cuttings. When cutting cuttings, the incision is required to be smooth, and the incision takes a long time to heal and is easy to rot. At the same time, the rooting time is longer; layering propagation is to press the branches of the plant close to the ground into the soil, and after it takes root, it is separated from the mother plant and planted to form a new plant. This method also has a longer propagation time and a longer rooting time. twig perishable problem

Method used

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  • A kind of tissue culture rapid propagation method of double poinsettia
  • A kind of tissue culture rapid propagation method of double poinsettia
  • A kind of tissue culture rapid propagation method of double poinsettia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of tissue culture rapid propagation method of poinsettia double petal described in the present embodiment, comprises the following steps:

[0029] (1) Selection and sterilization of explants: cut young leaves of poinsettia double petals, soak them in detergent for 10 minutes, and rinse them with running water; treat them with 75% alcohol for 20 seconds on an ultra-clean workbench, rinse them with sterile water for 3 times, Then treat with 0.1% mercuric chloride for 7 minutes, rinse with sterile water for 5 times, and dry to obtain explants;

[0030] (2) Callus induction culture: inoculate the explant described in step (1) into the induction medium, cultivate under the conditions of light intensity 2000Lx and light time 14h / d until callus is generated, and the induction culture The composition of the base is: MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, 0.5mg / L 6-BA, 1.0mg / L 2,4-D, 0.1mg / L NAA;

[0031] (3) Differentiation culture: inoculate the...

Embodiment 2

[0035] The tissue culture rapid propagation method of a kind of poinsettia double petal described in the present embodiment, the difference with embodiment 1 is:

[0036] The composition of step (2) induction medium is: MS minimal medium, supplemented with 25g / L sucrose, 5.5g / L agar, 0.1mg / L 6-BA, 0.5mg / L 2,4-D, 0.1mg / L NAA;

[0037] Step (3) The composition of the differentiation medium is: MS basic medium supplemented with 25g / L sucrose, 5.5g / L agar, 0.01mg / L TDZ, 0.5mg / L 6-BA;

[0038] The composition of step (4) rooting medium is: 1 / 2MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, 0.1mg / L IBA.

[0039] In the present embodiment, after step (2) induced culture for 3 weeks, callus formed, and the callus induction rate was 55%; after step (3) cultured in the differentiation medium for 20 days, there were adventitious buds gradually differentiated, and the culture was continued for 2 days. About a week, the adventitious buds grow to 1-3cm, the differentiation ...

Embodiment 3

[0041]The tissue culture rapid propagation method of a kind of poinsettia double petal described in the present embodiment, the difference with embodiment 1 is:

[0042] Step (2) The composition of the induction medium is: MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, 1.0mg / L 6-BA, 1.5mg / L 2,4-D, 0.5mg / L NAA;

[0043] Step (3) The composition of the differentiation medium is: MS basic medium supplemented with 25g / L sucrose, 5.5g / L agar, 0.10mg / L TDZ, 2.0mg / L 6-BA;

[0044] Step (4) The composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, and 0.5mg / L IBA.

[0045] In this example, after step (2) induced culture for 3 weeks, callus formed, and the callus induction rate was 66.7%; after step (3) cultured in the differentiation medium for 3 weeks, adventitious buds gradually differentiated, and the culture was continued In about 2 weeks, the adventitious buds grew to 1-2cm, and the differentiation rate was 40%....

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method of Euphorbia pulcherrima. The tissue culture and rapid propagation method comprises: (1) explant selecting and sterilizing: cutting the young and tender leaves of Euphorbia pulcherrima, soaking with a detergent, cleaning, and carrying out sterilization treatment with alcohol and mercury bichloride to obtain explant; (2) callus induction culture: inoculating the explant obtained in the step (1) into an induction culture medium, and culturing for 4-7 weeks until the callus is generated; (3) differentiation culture: inoculatingthe callus obtained in the step (2) into a differentiation culture medium, and culturing for 3-5 weeks until the differentiation and growth of adventitious buds occurs; and (4) rooting culture: inoculating the adventitious buds obtained in the step (3) into a rooting culture medium, culturing for 3-4 weeks until roots are generated from the root portions of the adventitious buds, and obtaining the Euphorbia pulcherrima tissue culture seedling after the root grows to achieve a certain length. According to the present invention, the tissue culture and rapid propagation method has advantages ofshort seedling culture time, high survival rate and high rooting rate, wherein the obtained seedlings are neat so as to conveniently perform the unified transplanting and planting management in the later period.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a method for tissue culture rapid propagation of poinsettia double petals. Background technique [0002] Double poinsettia is a variant of poinsettia, with red bracts, flat outer bracts and upright inner bracts, very beautiful, with high ornamental value, especially suitable for flower arrangement. Under normal circumstances, the bracts of the double poinsettia turn red in early November. [0003] The double poinsettia grows weaker than the common poinsettia, and the stem is thinner, which makes it difficult to take root. The existing double-petalled poinsettias are generally propagated by cuttings or layering. Cuttings mainly include semi-hard branch cuttings, tender branch cuttings, and old root cuttings. When cutting cuttings, the incision is required to be smooth, and the incision takes a long time to heal and is easy to rot. At the same time,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李飞曹亚琼曾俊罗琳
Owner SICHUAN COLORLINK CO LTD
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