A kind of tissue culture rapid propagation method of double poinsettia
A technology of tissue culture rapid propagation and poinsettia, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of long healing time of incisions, long rooting time, perishable branches, etc., and achieves the convenience of transplanting and planting The effects of management, neat seedlings, and shortened growth cycle
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Embodiment 1
[0028] A kind of tissue culture rapid propagation method of poinsettia double petal described in the present embodiment, comprises the following steps:
[0029] (1) Selection and sterilization of explants: cut young leaves of poinsettia double petals, soak them in detergent for 10 minutes, and rinse them with running water; treat them with 75% alcohol for 20 seconds on an ultra-clean workbench, rinse them with sterile water for 3 times, Then treat with 0.1% mercuric chloride for 7 minutes, rinse with sterile water for 5 times, and dry to obtain explants;
[0030] (2) Callus induction culture: inoculate the explant described in step (1) into the induction medium, cultivate under the conditions of light intensity 2000Lx and light time 14h / d until callus is generated, and the induction culture The composition of the base is: MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, 0.5mg / L 6-BA, 1.0mg / L 2,4-D, 0.1mg / L NAA;
[0031] (3) Differentiation culture: inoculate the...
Embodiment 2
[0035] The tissue culture rapid propagation method of a kind of poinsettia double petal described in the present embodiment, the difference with embodiment 1 is:
[0036] The composition of step (2) induction medium is: MS minimal medium, supplemented with 25g / L sucrose, 5.5g / L agar, 0.1mg / L 6-BA, 0.5mg / L 2,4-D, 0.1mg / L NAA;
[0037] Step (3) The composition of the differentiation medium is: MS basic medium supplemented with 25g / L sucrose, 5.5g / L agar, 0.01mg / L TDZ, 0.5mg / L 6-BA;
[0038] The composition of step (4) rooting medium is: 1 / 2MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, 0.1mg / L IBA.
[0039] In the present embodiment, after step (2) induced culture for 3 weeks, callus formed, and the callus induction rate was 55%; after step (3) cultured in the differentiation medium for 20 days, there were adventitious buds gradually differentiated, and the culture was continued for 2 days. About a week, the adventitious buds grow to 1-3cm, the differentiation ...
Embodiment 3
[0041]The tissue culture rapid propagation method of a kind of poinsettia double petal described in the present embodiment, the difference with embodiment 1 is:
[0042] Step (2) The composition of the induction medium is: MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, 1.0mg / L 6-BA, 1.5mg / L 2,4-D, 0.5mg / L NAA;
[0043] Step (3) The composition of the differentiation medium is: MS basic medium supplemented with 25g / L sucrose, 5.5g / L agar, 0.10mg / L TDZ, 2.0mg / L 6-BA;
[0044] Step (4) The composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 25g / L sucrose, 5.5g / L agar, and 0.5mg / L IBA.
[0045] In this example, after step (2) induced culture for 3 weeks, callus formed, and the callus induction rate was 66.7%; after step (3) cultured in the differentiation medium for 3 weeks, adventitious buds gradually differentiated, and the culture was continued In about 2 weeks, the adventitious buds grew to 1-2cm, and the differentiation rate was 40%....
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