The invention relates to the technical field of fertilizer processing, in particular to a leaf fertilizer used for grape planting and a preparation method thereof. The leaf fertilizer is prepared fromthe raw materials such as animal manure, mushroom residues, residues of furfural, soybean meal, tomato seedlings, tulip, euphorbia mili, poinsettia, Japanese basil, Sichuan peppers, saururaceae, calendula, stevia rebaudiana, urea, potassium phosphate, iron molybdate, kresoxim-methyl, auxiliaries, acidic substances and microorganisms through raw material treatment, fermentation, filtration, mixingand the like. The grape leaf fertilizer is rich in nutrient ingredient, has insecticidal sterilization effect, can effectively avoid animals such as ants, birds and mice, reduces harm, on fruits, ofplant diseases and insect pests, mice, birds and ants, reduces a pesticide and chemical fertilizer use rate, remarkably increases acre yield, soluble solid content and sugar content of grapes, is highin nutrient value, can reduce astringent of grapes, enables fruits to be big, full of water, and delicious; and the preparation process is simple, and is easy for production.